FastQCFastQC Report
Fri 17 Jun 2016
SRR1526564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35546
Sequences flagged as poor quality0
Sequence length64
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5441.5304112980363473No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.6048500534518652No Hit
TGAAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2050.5767174928261971No Hit
TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.36572328813368593No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.3066449108197828No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC890.25037978956844653No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.2109942046925111No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG720.20255443650481064No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGC680.19130141225454342No Hit
TGAAGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.17723513194170934No Hit
TGAAGCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1350362910032071No Hit
TGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.12659652281550665No Hit
TGAAGCGGGATCTCCACAGCGCTATGCACACATCTTTCCTAGTTAGAGTAAAATCAGAAAGAAA430.12097001069037304No Hit
TGAAGCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1125302425026726No Hit
TGAAGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1125302425026726No Hit
TGAAGCGGGATTTCAGGAATTTCAATCTTAAGGAAAAAGTAACAAAATAACAATTTCCCATTTT390.10971698644010577No Hit
TGAAGCGGGATATCTATGTGATTAGCCCAGGGAAACTAAGGCTCAGACATGGATAATACCACAG360.10127721825240532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGCAT150.00452682958.3931636
TGGCATG150.004552467358.31010437
AAACTGG201.9319578E-458.31010439
GCATGCT150.004552467358.31010439
TCCGGGC201.9456193E-458.22727632
AGGACGT150.004604068658.14468456
TTCCGGG201.9593575E-458.14468431
ATAATTA150.004604068658.14468458
GACGTGT150.004604068658.14468458
TGAAGGA150.004604068658.14468453
ATTCCGG201.9593575E-458.14468430
GGACGTG150.004604068658.14468457
GAAGGAC150.004604068658.14468454
CGATGAA150.00463003258.06232550
AGCCGAT150.00463003258.06232547
ACGGTGA150.00463003258.06232548
GCCGATG150.00463003258.06232548
GCATTGG150.00463003258.06232546
CCGATGA150.00463003258.06232549
TGAGCCG150.00463003258.06232545
Produced by FastQC (version 0.11.5)