FastQCFastQC Report
Fri 17 Jun 2016
SRR1526563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31147
Sequences flagged as poor quality0
Sequence length56
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT6732.1607217388512536No Hit
TGAAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA3171.0177545188942756No Hit
TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.8186984300253636No Hit
TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.5907471024496741No Hit
TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.375638103188108No Hit
TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.3017947153818987No Hit
TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA790.2536359842039362No Hit
TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA690.2215301634186278No Hit
TGAAGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.19263492471185026No Hit
TGAAGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.17337143224066523No Hit
TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA520.16695026808360355No Hit
TGAAGCGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT520.16695026808360355No Hit
TGAAGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG510.16373968600507272No Hit
TGAAGCGGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.16052910392654188No Hit
TGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1541079397694802No Hit
TGAAGCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA440.14126561145535688No Hit
TGAAGCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.13805502937682604No Hit
TGAAGCGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA420.13484444729829517No Hit
TGAAGCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1284232831412335No Hit
TGAAGCGGGATATCTATGTGATTAGCCCAGGGAAACTAAGGCTCAGACATGGATAA390.12521270106270266No Hit
TGAAGCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.12200211898417182No Hit
TGAAGCGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGC350.11237037274857932No Hit
TGAAGCGGGACAATGGTACAACGCAGAGTCGACTCTCTTTTTTTTTTTTTTTTTTT350.11237037274857932No Hit
TGAAGCGGGGAACTTTTCACTGTCTTGTGGTCATCTGGTCTCCTCATTATTTTTAT330.10594920859151764No Hit
TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC330.10594920859151764No Hit
TGAAGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGA330.10594920859151764No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAAC150.0079527850.62520636
TGCGATT150.0079527850.62520636
CCCGCTG150.0079527850.62520635
GCAAACT150.0079527850.62520637
ATTGCGA150.0079527850.62520634
TTGCGAT150.0079527850.62520635
TTCCGGG203.937939E-450.46003731
TCCGGGC203.937939E-450.46003732
CCGGGCA203.937939E-450.46003733
GTATGCG150.00805643250.46003331
AAACTGG150.0082137950.21428739
ATTGCGG150.0082137950.21428740
TCTCTCC150.0082137950.21428739
CAAACTG150.0082137950.21428738
GAACACC150.00826674750.13290447
CCGCGAT150.00831995750.0517829
TTGCAGG150.00831995750.0517828
AACCGCG150.00831995750.0517827
ACGTATG204.0995202E-450.0517829
ACCGCGA150.00831995750.0517828
Produced by FastQC (version 0.11.5)