##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526563_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 31147 Sequences flagged as poor quality 0 Sequence length 56 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.397341638038974 39.0 38.0 40.0 35.0 40.0 2 37.95026808360356 39.0 38.0 40.0 35.0 40.0 3 37.71785404693871 39.0 38.0 40.0 33.0 40.0 4 38.19687289305551 39.0 38.0 40.0 35.0 40.0 5 37.70324589848139 39.0 38.0 40.0 34.0 40.0 6 37.801521815905225 39.0 38.0 40.0 35.0 40.0 7 38.18043471281343 39.0 38.0 40.0 36.0 40.0 8 38.37563810318811 39.0 38.0 40.0 36.0 40.0 9 38.30991748804058 39.0 38.0 40.0 36.0 40.0 10 37.048897165056026 39.0 36.0 40.0 32.0 40.0 11 37.34411018717694 39.0 37.0 40.0 33.0 40.0 12 37.27440845025203 39.0 37.0 40.0 33.0 40.0 13 37.2126368510611 39.0 37.0 40.0 33.0 40.0 14 37.22515812116737 39.0 37.0 40.0 33.0 40.0 15 37.25228753973095 39.0 37.0 40.0 33.0 40.0 16 37.086621504478764 39.0 36.0 40.0 33.0 40.0 17 37.13699553729091 39.0 37.0 40.0 33.0 40.0 18 37.02850996885736 39.0 36.0 40.0 33.0 40.0 19 36.815134683918195 39.0 36.0 40.0 32.0 40.0 20 37.003178476257744 39.0 36.0 40.0 33.0 40.0 21 36.87562205027771 38.0 36.0 40.0 33.0 40.0 22 36.61871127235368 38.0 36.0 40.0 31.0 40.0 23 36.407968664718915 38.0 36.0 40.0 31.0 40.0 24 36.286223392301025 38.0 35.0 40.0 31.0 40.0 25 36.34751340418018 38.0 35.0 40.0 31.0 40.0 26 36.08302565255081 38.0 35.0 39.0 30.0 40.0 27 36.08276880598452 38.0 35.0 39.0 30.0 40.0 28 35.818441583459084 38.0 35.0 39.0 29.0 40.0 29 35.91678171252448 38.0 35.0 39.0 30.0 40.0 30 35.77689665136289 38.0 35.0 39.0 29.0 40.0 31 35.67695123125823 39.0 35.0 40.0 29.0 40.0 32 35.42193469676052 39.0 35.0 39.0 28.0 40.0 33 35.56233345105468 39.0 35.0 40.0 29.0 40.0 34 35.742254470735546 39.0 35.0 40.0 29.0 40.0 35 35.52637493177513 39.0 35.0 40.0 29.0 40.0 36 35.42106783959932 39.0 35.0 40.0 29.0 40.0 37 34.91508010402286 39.0 35.0 40.0 26.0 40.0 38 35.09442321892959 39.0 35.0 40.0 27.0 40.0 39 35.084823578514786 39.0 35.0 40.0 27.0 40.0 40 34.72716473496645 39.0 35.0 40.0 25.0 40.0 41 34.61383118759431 39.0 35.0 39.0 25.0 40.0 42 34.58702282723858 39.0 35.0 39.0 25.0 40.0 43 34.42578739525476 39.0 35.0 39.0 24.0 40.0 44 33.9278903265162 39.0 34.0 39.0 19.0 40.0 45 33.92811506726169 39.0 34.0 39.0 21.0 40.0 46 33.545188942755324 39.0 34.0 39.0 13.0 40.0 47 33.40177224130735 39.0 34.0 39.0 10.0 40.0 48 33.021607217388514 38.0 33.0 39.0 5.0 40.0 49 32.97845699425306 38.0 33.0 39.0 4.0 40.0 50 32.622563970847914 38.0 33.0 39.0 4.0 40.0 51 32.03913699553729 38.0 33.0 39.0 4.0 40.0 52 31.753812566218254 38.0 32.0 39.0 4.0 40.0 53 31.331942081099303 38.0 32.0 39.0 4.0 40.0 54 30.840241435772306 37.0 31.0 39.0 4.0 40.0 55 30.185764279063793 37.0 30.0 39.0 4.0 40.0 56 17.72430731691656 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.3171112344690172 1 2 -0.8186196767753913 1 3 -0.9329241717787298 1 4 -1.773244639688464 1 5 -0.736488962302019 1 6 -1.0890646849929553 1 7 -2.2648237993127935 1 8 -2.522659513547346 1 9 -2.960075742085735 1 10 -1.34191925065317 1 11 -2.932678146044225 1 12 -1.0801242752813707 1 13 -1.876436438570387 1 14 -0.9762159826143275 1 15 -2.2464832756062876 1 16 -2.5263752159938733 1 17 -1.304090220360024 1 18 -1.1508846956385739 1 19 -2.431058809593928 1 20 -1.7090836954292783 1 21 -2.0105270268219257 1 22 -1.8862832863810155 1 23 -2.2507422277147455 1 24 -1.8962222462525347 1 25 -2.1798814069368433 1 26 -0.7202312841028728 1 27 -1.2813850410304823 1 28 -0.46250932510452003 1 29 -2.3914662824271957 1 30 -0.7993982699130058 1 31 -3.124712216983724 1 32 -2.836150640388155 1 33 -2.1448525931971005 1 34 -2.8697860031156353 1 35 -2.714784043238687 1 36 -1.6939436731827442 1 37 -1.862440099861729 1 38 -1.283655055357407 1 39 -1.667214254674441 1 40 -2.161160055411713 1 41 -1.898066270686897 1 42 -2.9765498798850274 1 43 -2.160578339604406 1 44 -2.5186505124152987 1 45 -2.27924779622289 1 46 -2.2340708683176693 1 47 -3.050232356444237 1 48 -1.9800362913230387 1 49 -3.2440264827774072 1 50 -1.7873732645253106 1 51 -2.61002581003018 1 52 -1.6493634911197006 1 53 -1.795387233598852 1 54 -1.083783438992409 1 55 -0.6769975223938296 1 56 -1.7580426694556035 2 1 0.7279868047466707 2 2 0.8284391467540217 2 3 0.38276210273107125 2 4 0.8169514387429047 2 5 1.0321384886783704 2 6 1.1677980601050777 2 7 0.9606663967656388 2 8 0.8322424472369647 2 9 0.7516889637966173 2 10 1.4482768277782014 2 11 -0.03856049898539737 2 12 0.27869925413039454 2 13 -0.7176129091586176 2 14 0.2649604879739016 2 15 0.2241049596878284 2 16 -0.07539482383700857 2 17 0.18218428944389586 2 18 0.3863702063222121 2 19 -0.015372535084125616 2 20 -0.2855542836645739 2 21 -0.6791544778023137 2 22 0.43136377244251634 2 23 -0.09780105124416139 2 24 0.8547581459043272 2 25 0.23384408325923545 2 26 -0.15748618606365739 2 27 -0.026483080246173074 2 28 1.0825887141111679 2 29 1.0908866587492767 2 30 1.3888370242046406 2 31 2.1654838614476475 2 32 2.0128689674549847 2 33 1.8041670146460405 2 34 1.7890375262961342 2 35 2.0028630155848433 2 36 2.119781817013333 2 37 2.1473638217069038 2 38 1.9614429838582836 2 39 1.832785745325559 2 40 2.8192321014510355 2 41 2.2842866704895712 2 42 2.448940316193408 2 43 2.825696170199514 2 44 3.4342906640552897 2 45 2.7972227920124055 2 46 3.4482820728588024 2 47 3.69486568277145 2 48 3.7081990027946077 2 49 3.563816654477492 2 50 3.306744382533516 2 51 3.935072229185508 2 52 4.052597293194022 2 53 4.06539708012664 2 54 3.2966087178703383 2 55 3.6504534579983314 2 56 0.7537220364267476 3 1 -0.6353908043614922 3 2 0.2297674199988009 3 3 -0.33830051586475207 3 4 0.12245428504272127 3 5 0.15168308070872882 3 6 -0.010570061337041636 3 7 0.28893964154742235 3 8 -0.09470252430003256 3 9 -0.5149144517631541 3 10 -0.2300912936639179 3 11 -0.5584845976571273 3 12 -1.0543178236684696 3 13 -1.2441783740542576 3 14 0.17862272706308602 3 15 -0.5045477917353196 3 16 0.032764568952366346 3 17 -0.009466564446043435 3 18 -1.0519599644557758 3 19 0.005500330191019032 3 20 0.11672275618362704 3 21 -0.5890216504778394 3 22 -0.3637026412197244 3 23 -0.6442906148115242 3 24 -1.0048243967901698 3 25 0.3287207436007904 3 26 0.5227794685852984 3 27 0.885281625636182 3 28 0.17082400822881283 3 29 0.6601466207986135 3 30 -0.5703660118484848 3 31 0.6322770303281047 3 32 0.469225703697866 3 33 1.2605237508889218 3 34 1.0044075452714623 3 35 1.28844176321293 3 36 2.0856262192903685 3 37 1.809602910890959 3 38 1.7916137618468966 3 39 2.542463164680399 3 40 2.1721732779216225 3 41 1.7890305035066518 3 42 2.6481813029106718 3 43 2.311464671148279 3 44 2.661994648875023 3 45 1.0562360747448523 3 46 -0.33729667476928427 3 47 0.8798751704374794 3 48 2.0877056441608346 3 49 1.7678014742118435 3 50 1.770691251603722 3 51 2.862017200722505 3 52 2.5753676916759964 3 53 2.8357955621000706 3 54 2.6656789265989893 3 55 3.2111745206169218 3 56 2.21292507247988 4 1 0.8162220988643156 4 2 1.7147136565579402 4 3 1.1004091615546017 4 4 0.8600886936448688 4 5 1.1635110376979796 4 6 1.3776019816737062 4 7 1.068509534020535 4 8 0.8106738197859897 4 9 0.8399242579142623 4 10 0.8914140826801642 4 11 1.200655187289108 4 12 0.5532090580519622 4 13 1.49023022809628 4 14 1.057117350719004 4 15 0.653516724393711 4 16 0.773624784006131 4 17 0.8292431129733089 4 18 0.9157819710280961 4 19 1.1022745237394034 4 20 0.7242496379040588 4 21 1.3561396398447414 4 22 1.8803833802856573 4 23 1.4159244389519188 4 24 2.1037777537474653 4 25 1.1867852597298238 4 26 2.5797687158971243 4 27 2.0519482923028463 4 28 2.5041573415621485 4 29 2.008533717572803 4 30 2.4339350634203285 4 31 1.1086211163496102 4 32 1.863849359611848 4 33 1.8551474068028995 4 34 1.930213996884369 4 35 0.65188262342798 4 36 1.53938966015059 4 37 2.0375599001382767 4 38 2.1163449446425986 4 39 2.2994524119922275 4 40 2.3055066112549554 4 41 2.8352670626464302 4 42 2.623450120114974 4 43 3.0727549937289282 4 44 3.5146828209180327 4 45 3.1207522037771085 4 46 3.399262465015667 4 47 1.0497676435557608 4 48 1.9866303753436299 4 49 1.7893068505559242 4 50 2.3459600688080258 4 51 1.0899741899698228 4 52 1.8173031755469644 4 53 0.7379460997344793 4 54 1.6162165610075938 4 55 -0.9103308557271603 4 56 -0.6913760027889388 5 1 0.6162220988643128 5 2 1.348046989891273 5 3 0.767075828221266 5 4 0.5267553603115331 5 5 1.4635110376979767 5 6 1.010935315007039 5 7 0.9351762006872022 5 8 0.7773404864526512 5 9 0.8732575912475937 5 10 0.42474741601349564 5 11 0.767321853955778 5 12 0.8532090580519593 5 13 0.7902302280962843 5 14 1.423784017385671 5 15 0.8535167243937138 5 16 0.5069581173394653 5 17 0.3292431129733089 5 18 1.4491153043614275 5 19 1.068941190406072 5 20 1.3242496379040531 5 21 1.8561396398447414 5 22 1.647050046952323 5 23 0.6825911056185845 5 24 0.2371110870807982 5 25 1.4201185930631581 5 26 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-0.09394367318274988 120 37 0.17089323347160956 120 38 -0.016988388690741374 120 39 0.2994524119922275 120 40 0.2388399445882925 120 41 0.5019337293131017 120 42 0.39011678678163975 120 43 0.5394216603955968 120 44 0.5813494875847027 120 45 -1.3125811295562215 120 46 0.1659291316823328 120 47 0.11643431022243078 120 48 0.25329704201029557 120 49 1.12264018388926 120 50 0.6126267354746915 120 51 0.6566408566364856 120 52 0.9839698422136287 120 53 0.4712794330678136 120 54 0.01621656100759239 120 55 0.6230024776061711 120 56 0.8086239972110612 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 26.0 5 5.0 6 3.0 7 10.0 8 34.0 9 39.0 10 23.0 11 25.0 12 34.0 13 46.0 14 95.0 15 90.0 16 110.0 17 111.0 18 114.0 19 130.0 20 116.0 21 196.0 22 192.0 23 175.0 24 212.0 25 282.0 26 289.0 27 332.0 28 443.0 29 464.0 30 633.0 31 919.0 32 1081.0 33 1312.0 34 1774.0 35 2251.0 36 3052.0 37 5066.0 38 6423.0 39 5040.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 100.0 0.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.9079112196062 0.8576108951915974 0.1573892654096939 0.07708861979250313 8 99.21661797283848 0.6581693260988217 0.04173756702090089 0.08347513404180178 9 96.0028253122291 2.4625164542331524 0.6934857289626609 0.8411725045750794 10 26.143769865476614 46.3094359007288 9.490480624137156 18.056313609657433 11 22.750184608469514 39.92358814653096 20.075769737053328 17.25045750794619 12 44.73625068224869 19.395126336404793 20.27161524384371 15.59700773750281 13 26.506565640350594 18.881433203839855 28.105435515458954 26.506565640350594 14 27.376633383632452 29.12640061643176 23.183613189071178 20.31335281086461 15 34.13169807686133 22.94924069733843 24.666902109352424 18.25215911644781 16 21.632902045140785 27.122997399428517 26.956047131344913 24.28805342408579 17 26.586830192313865 25.719973031110538 20.84630943590073 26.84688734067486 18 26.48088098372235 29.51809163001252 21.642533791376376 22.358493594888753 19 19.96982052846181 29.66256782354641 27.068417504093496 23.299194143898287 20 22.403441743988182 30.333579477959354 22.663498892349182 24.59947988570328 21 22.419494654380838 24.249526439143416 28.050855620123926 25.280123286351813 22 24.769640735865412 24.898064019006647 24.782483064179537 25.549812180948408 23 25.787395254759687 30.484476835650305 24.727903168844513 19.000224740745498 24 21.32147558352329 33.9615372266992 25.280123286351813 19.436863903425692 25 22.847139691003115 23.666206276298464 30.790479555455626 22.6961744772428 26 23.03466443923282 22.041957143316093 33.3311915700196 21.592186847431492 27 17.403181745139 19.874658524827254 37.03358508757834 25.688574642455407 28 16.332433474739684 24.78146291297082 41.811286797788924 17.07481681450058 29 18.77772776956214 19.67144602327525 44.30013502218221 17.25069118498039 30 13.633877143868457 24.600186633201403 47.0058242430093 14.760111979920843 31 15.147030435621902 21.57518821182678 50.90084293160029 12.376938420951031 32 9.628389257422555 18.702904617762606 58.446576930508144 13.222129194306692 33 10.896382903340097 19.699165780912807 55.28714529584179 14.117306019905303 34 11.664465418935025 20.065715298134844 57.49444319170184 10.775376091228296 35 9.476397615595296 23.231835024971804 57.46415337522153 9.827613984211373 36 9.552046406703191 19.961327747341283 59.07508862391234 11.411537222043185 37 9.859887519911577 18.8843015506648 61.49344949774065 9.762361431682976 38 9.015547384039738 19.51809560673505 61.96374427456293 9.50261273466228 39 8.959267262231108 19.534298706743638 62.027929177282545 9.478504853742702 40 9.857516719976738 19.634260605473166 61.68782914930051 8.820393525249589 41 8.46593293096214 20.469289610431527 62.66339605590162 8.40138140270471 42 9.401240149851441 18.766955173750162 61.24854669939285 10.583257977005553 43 10.650811090286306 19.385380986234086 61.04181477412266 8.921993149356943 44 9.566763659877143 19.776915615906887 61.80407371483996 8.85224700937601 45 10.62364618020756 22.352979211793993 59.37085771556044 7.652516892438007 46 9.757438551099613 21.57503234152652 59.03945666235446 9.628072445019404 47 9.653009087087282 22.59806616434369 59.88099472884261 7.867930019726417 48 11.877084480134702 22.536670660233785 57.934786128290646 7.651458731340867 49 9.800938663214112 23.987700275125427 58.31364298430167 7.897718077358797 50 9.19536509580528 23.44963749352667 58.972035214914555 8.382962195753496 51 9.970551114850652 23.51056600110029 58.706837966408855 7.812044917640206 52 9.838772338772339 23.672623672623672 57.36531986531986 9.123284123284122 53 9.98251521823598 24.92552778137547 56.26861805465613 8.823338945732417 54 10.334887938852184 25.382173856717195 55.492939499935225 8.789998704495401 55 10.67668685821645 26.332156392731044 53.21499141589193 9.776165333160572 56 10.333883869296285 26.82405518313417 52.45960037565983 10.38246057190971 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 4.0 9 8.0 10 35.5 11 63.0 12 61.5 13 86.5 14 113.0 15 151.5 16 190.0 17 358.0 18 526.0 19 699.0 20 872.0 21 936.5 22 1147.5 23 1294.0 24 1610.5 25 1927.0 26 2219.5 27 2512.0 28 2715.5 29 2827.5 30 2736.0 31 2652.5 32 2569.0 33 2058.0 34 1547.0 35 1596.0 36 1645.0 37 1176.0 38 620.0 39 533.0 40 520.0 41 507.0 42 503.5 43 500.0 44 569.5 45 639.0 46 647.5 47 586.0 48 516.0 49 507.0 50 498.0 51 623.0 52 748.0 53 930.0 54 1036.0 55 960.0 56 906.0 57 852.0 58 820.0 59 788.0 60 704.5 61 621.0 62 535.5 63 399.0 64 348.0 65 299.5 66 251.0 67 209.0 68 167.0 69 139.5 70 112.0 71 99.0 72 57.0 73 28.0 74 29.5 75 31.0 76 23.0 77 15.0 78 13.5 79 15.5 80 19.0 81 12.0 82 5.0 83 4.0 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.044948149099431726 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.044948149099431726 26 0.06421164157061675 27 0.1027386265129868 28 0.09952804443445597 29 0.13163386521976433 30 0.22474074549715864 31 0.20868783510450445 32 0.2985841333033679 33 0.32105820785308375 34 0.3339005361672071 35 0.3595851927954538 36 0.375638103188108 37 1.2392846823129033 38 0.4655344013869715 39 0.4494814909943173 40 0.6292740873920442 41 0.5265354608790573 42 0.5875365203711432 43 0.6453269977846984 44 0.6966963110411918 45 0.6934857289626609 46 0.7288021318265001 47 0.7191703855909076 48 0.8475936687321411 49 0.8090666837897711 50 0.8058561017112402 51 0.789803191318586 52 0.8315407583394869 53 0.8443830866536103 54 0.870067743281857 55 0.8861206536745112 56 0.8604359970462645 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 31147.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.929463511734674 #Duplication Level Percentage of deduplicated Percentage of total 1 73.85740402193784 37.61517963206729 2 11.775830549076467 11.99473464539121 3 4.872974847128538 7.445339840113012 4 2.6602786358192017 5.4194625485600545 5 1.746201853369476 4.44665617876521 6 1.204059761709639 3.6793270619963394 7 0.7943012040597617 2.831733393264199 8 0.6871335812897938 2.79962757247889 9 0.4412784466998676 2.0226667094744277 >10 1.8785853873794365 15.14110508235143 >50 0.050431822479984866 1.640607442129258 >100 0.025215911239992433 2.802838154557421 >500 0.006303977809998108 2.1607217388512536 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TGAAGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 673 2.1607217388512536 No Hit TGAAGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 317 1.0177545188942756 No Hit TGAAGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.8186984300253636 No Hit TGAAGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.5907471024496741 No Hit TGAAGCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.375638103188108 No Hit TGAAGCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 0.3017947153818987 No Hit TGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 79 0.2536359842039362 No Hit TGAAGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 69 0.2215301634186278 No Hit TGAAGCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.19263492471185026 No Hit TGAAGCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.17337143224066523 No Hit TGAAGCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 52 0.16695026808360355 No Hit TGAAGCGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT 52 0.16695026808360355 No Hit TGAAGCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 51 0.16373968600507272 No Hit TGAAGCGGGTACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 50 0.16052910392654188 No Hit TGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.1541079397694802 No Hit TGAAGCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 44 0.14126561145535688 No Hit TGAAGCGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.13805502937682604 No Hit TGAAGCGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.13484444729829517 No Hit TGAAGCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.1284232831412335 No Hit TGAAGCGGGATATCTATGTGATTAGCCCAGGGAAACTAAGGCTCAGACATGGATAA 39 0.12521270106270266 No Hit TGAAGCGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38 0.12200211898417182 No Hit TGAAGCGGGCATGCCATGGTGAAAAAAACCGCGATTGCGATTGCGGTGGCGCTGGC 35 0.11237037274857932 No Hit TGAAGCGGGACAATGGTACAACGCAGAGTCGACTCTCTTTTTTTTTTTTTTTTTTT 35 0.11237037274857932 No Hit TGAAGCGGGGAACTTTTCACTGTCTTGTGGTCATCTGGTCTCCTCATTATTTTTAT 33 0.10594920859151764 No Hit TGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC 33 0.10594920859151764 No Hit TGAAGCGGGGATGAGATGGCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGA 33 0.10594920859151764 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0032105820785308377 0.0 0.0 0.0 0.0 9 0.0032105820785308377 0.0 0.0 0.0 0.0 10 0.019263492471185025 0.0 0.0 0.0 0.0 11 0.022474074549715863 0.0 0.0 0.0 0.0 12 0.022474074549715863 0.0 0.0 0.0 0.0 13 0.022474074549715863 0.0 0.0 0.0 0.0 14 0.03852698494237005 0.0 0.0 0.0 0.0 15 0.04173756702090089 0.0 0.0 0.0 0.0 16 0.044948149099431726 0.0 0.0 0.0 0.0 17 0.05457989533502424 0.0 0.0 0.0 0.0 18 0.06421164157061675 0.0 0.0 0.0 0.0 19 0.06421164157061675 0.0 0.0 0.0 0.0 20 0.08026455196327094 0.0 0.0 0.0 0.0 21 0.08989629819886345 0.0 0.0 0.0 0.0 22 0.09310688027739429 0.0 0.0 0.0 0.0 23 0.09952804443445597 0.0 0.0 0.0 0.0 24 0.118791536905641 0.0 0.0 0.0 0.0 25 0.12200211898417183 0.0 0.0 0.0 0.0 26 0.13484444729829517 0.0 0.0 0.0 0.0 27 0.138055029376826 0.0 0.0 0.0 0.0 28 0.1541079397694802 0.0 0.0 0.0 0.0 29 0.16052910392654188 0.0 0.0 0.0 0.0 30 0.19263492471185026 0.0 0.0 0.0 0.0 31 0.19905608886891193 0.0 0.0 0.0 0.0 32 0.19905608886891193 0.0 0.0 0.0 0.0 33 0.20226667094744277 0.0 0.0 0.0 0.0 34 0.21831958134009696 0.0 0.0 0.0 0.0 35 0.2536359842039362 0.0 0.0 0.0 0.0 36 0.256846566282467 0.0 0.0 0.0 0.0 37 0.26326773043952867 0.0 0.0 0.0 0.0 38 0.26647831251805953 0.0 0.0 0.0 0.0 39 0.2793206408321829 0.0 0.0 0.0 0.0 40 0.29216296914630624 0.0 0.0 0.0 0.0 41 0.3017947153818987 0.0 0.0 0.0 0.0 42 0.3082158795389604 0.0 0.0 0.0 0.0 43 0.32747937201014543 0.0 0.0 0.0 0.0 44 0.3306899540886763 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAAAC 15 0.00795278 50.625206 36 TGCGATT 15 0.00795278 50.625206 36 CCCGCTG 15 0.00795278 50.625206 35 GCAAACT 15 0.00795278 50.625206 37 ATTGCGA 15 0.00795278 50.625206 34 TTGCGAT 15 0.00795278 50.625206 35 TTCCGGG 20 3.937939E-4 50.460037 31 TCCGGGC 20 3.937939E-4 50.460037 32 CCGGGCA 20 3.937939E-4 50.460037 33 GTATGCG 15 0.008056432 50.460033 31 AAACTGG 15 0.00821379 50.214287 39 ATTGCGG 15 0.00821379 50.214287 40 TCTCTCC 15 0.00821379 50.214287 39 CAAACTG 15 0.00821379 50.214287 38 GAACACC 15 0.008266747 50.132904 47 CCGCGAT 15 0.008319957 50.05178 29 TTGCAGG 15 0.008319957 50.05178 28 AACCGCG 15 0.008319957 50.05178 27 ACGTATG 20 4.0995202E-4 50.05178 29 ACCGCGA 15 0.008319957 50.05178 28 >>END_MODULE