FastQCFastQC Report
Fri 17 Jun 2016
SRR1526562_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526562_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32322
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5211.611905203885898No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4321.3365509560051976No Hit
AAAGTTGGGGGTCGTGTGGTGGGGAGAAGAGGACAGGGCGGATCGTGAGCCGAGCAGGCCACCG2680.829156611595817No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1950.6033042509745684No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.49811274054823335No Hit
AAAGTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.3557948146773096No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1150.3557948146773096No Hit
AAAGTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG910.2815419837881319No Hit
AAAGTTGGGCATGCTGGCCCTCTCTGGGTATGTCCAGGGCTGCCGGGCTCTCATGATCACCGCC900.27844811583441614No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.2629787760658375No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.23204009652868016No Hit
AAAGTTGGGGTGATGTGGGTGTGGTACCCGAGTAGCGCGGGACTCGTGAAATCTCGTGTGAATC710.21966462471381723No Hit
AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC690.2134768888063857No Hit
AAAGTTGGGAATTCTCAAGCAAAGATACCGCCTGACTCGGAGCTAAAGTTGGCTGACCTGCTCA650.20110141699152279No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.18872594517665986No Hit
AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG590.1825382092692284No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.1670688695006497No Hit
AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.16397500154693398No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.16088113359321823No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.15778726563950252No Hit
AAAGTTGGGGATTCCAGTAGTATTTATGTTGAAAGAAGCAAATTTTAGTCCTTCCAACTGTGGA470.14541179382463956No Hit
AAAGTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT470.14541179382463956No Hit
AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG460.14231792587092384No Hit
AAAGTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG460.14231792587092384No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.1330363220097766No Hit
AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.1299424540560609No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.12684858610234515No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA400.12375471814862941No Hit
AAAGTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12066085019491368No Hit
AAAGTTGGGATGGCGAAAGAGATCGGTGCTGTCAAATACCTGGAGTGCTCAGCTCTCACACAGC380.11756698224119795No Hit
AAAGTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.11447311428748222No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.10828537838005073No Hit
AAAGTTGGGATTCATAAGTACTATGGGTATCACAGTAGACTCACAGCTAATTTGAAATCCCAAG340.105191510426335No Hit
AAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.105191510426335No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.105191510426335No Hit
AAAGTTGGGATCTTCTATGTTAATATGTACGTCAGCATCGTGTTCTTTGGGCTCATCAGCTTTG340.105191510426335No Hit
AAAGTTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.10209764247261927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAGAC303.550067E-758.2262152
GAGCCGA258.286281E-658.2262147
TCGGAGA303.550067E-758.2262151
GACACGC303.550067E-758.2262156
AGGCCAC258.286281E-658.2262156
GGCCACC258.286281E-658.2262157
CGAGCAG258.286281E-658.2262151
CCGAGCA258.286281E-658.2262150
CAGGCCA258.286281E-658.2262155
AGCCGAG258.286281E-658.2262148
AGACACG303.550067E-758.2262155
TGAGTCG303.550067E-758.2262147
GTCGGAG303.550067E-758.2262150
TTTGATC201.9431453E-458.22620849
CTGAGTC303.5887024E-758.13551746
AATTTTA150.004603189458.13551741
GATCGTG258.363493E-658.13551741
AGGGCGG258.363493E-658.13551735
ACTCTAC258.363493E-658.13551736
GCGGATC258.363493E-658.13551738
Produced by FastQC (version 0.11.5)