FastQCFastQC Report
Fri 17 Jun 2016
SRR1526561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30738
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4281.3924132994989913No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3601.1711887565879369No Hit
AAAGTTGGGGGTCGTGTGGTGGGGAGAAGAGGACAGGGCGGATCGTGAGCCGAGCAGGCCACCG2150.699459951851129No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1550.5042618257531394No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.3611165332812805No Hit
AAAGTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG740.24074435552085366No Hit
AAAGTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.23423775131758734No Hit
AAAGTTGGGGTGATGTGGGTGTGGTACCCGAGTAGCGCGGGACTCGTGAAATCTCGTGTGAATC700.22773114711432105No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.22447784501268786No Hit
AAAGTTGGGCATGCTGGCCCTCTCTGGGTATGTCCAGGGCTGCCGGGCTCTCATGATCACCGCC660.2147179387077884No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.18869152189472316No Hit
AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC570.18543821979309No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.17893161558982368No Hit
AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.15941180298002472No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1561585008783916No Hit
AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG480.1561585008783916No Hit
AAAGTTGGGAATTCTCAAGCAAAGATACCGCCTGACTCGGAGCTAAAGTTGGCTGACCTGCTCA470.1529051987767584No Hit
AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG460.14965189667512524No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.14314529247185895No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1301320840653263No Hit
AAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12687878196369315No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12687878196369315No Hit
AAAGTTGGGGATTCCAGTAGTATTTATGTTGAAAGAAGCAAATTTTAGTCCTTCCAACTGTGGA380.12362547986206No Hit
AAAGTTGGGATGGCGAAAGAGATCGGTGCTGTCAAATACCTGGAGTGCTCAGCTCTCACACAGC380.12362547986206No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.11711887565879367No Hit
AAAGTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.10410566725226103No Hit
AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCC310.1008523651506279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACAG406.4210326E-1058.3690330
GAGGACA406.530172E-1058.27302629
GGGCGGA406.530172E-1058.27302636
CTCCTGT150.004558007758.2730237
AGCTGCT150.004558007758.2730239
GTCCAGG150.004587833358.17733832
ACTGAGT258.39924E-658.08196645
GATCGTG406.712071E-1058.08196641
GAAGAGG406.712071E-1058.08196626
TCGTGAG406.712071E-1058.08196643
GTGAGCC406.712071E-1058.08196645
GACACGC258.39924E-658.08196656
AGCAGGC406.712071E-1058.08196653
AGGCCAC406.712071E-1058.08196656
GGCCACC406.712071E-1058.08196657
CGAGCAG406.712071E-1058.08196651
CCGAGCA406.712071E-1058.08196650
CAGGCCA406.712071E-1058.08196655
ATCGTGA406.712071E-1058.08196642
CGTGAGC406.712071E-1058.08196644
Produced by FastQC (version 0.11.5)