##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526561_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30738 Sequences flagged as poor quality 0 Sequence length 64 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.8768950484742 39.0 38.0 40.0 34.0 40.0 2 37.67479992192075 39.0 38.0 40.0 33.0 40.0 3 37.49938187260069 39.0 38.0 40.0 33.0 40.0 4 37.29266705706292 39.0 38.0 40.0 33.0 40.0 5 36.77633548051272 39.0 36.0 40.0 31.0 40.0 6 37.75782419155443 39.0 38.0 40.0 34.0 40.0 7 37.56571670245299 39.0 38.0 40.0 34.0 40.0 8 37.46886589888737 39.0 38.0 40.0 33.0 40.0 9 37.61490663022968 39.0 38.0 40.0 34.0 40.0 10 36.96424620990305 39.0 36.0 40.0 32.0 40.0 11 37.263452404190254 39.0 37.0 40.0 33.0 40.0 12 37.13182380115818 39.0 36.0 40.0 32.0 40.0 13 36.95214392608498 39.0 36.0 40.0 32.0 40.0 14 37.17206714815538 39.0 37.0 40.0 33.0 40.0 15 36.87816383629384 39.0 36.0 40.0 32.0 40.0 16 36.69545839026612 39.0 36.0 40.0 31.0 40.0 17 36.43089986336131 39.0 36.0 40.0 31.0 40.0 18 36.71927256165007 39.0 36.0 40.0 31.0 40.0 19 36.671481553777085 39.0 36.0 40.0 31.0 40.0 20 36.69236775326957 39.0 36.0 40.0 31.0 40.0 21 36.76111002667708 39.0 36.0 40.0 32.0 40.0 22 36.574370486043335 39.0 36.0 40.0 31.0 40.0 23 36.435194222135465 38.0 36.0 40.0 31.0 40.0 24 36.25030906369965 38.0 35.0 40.0 30.0 40.0 25 36.169171709284925 38.0 35.0 40.0 30.0 40.0 26 36.310234888411735 39.0 36.0 40.0 30.0 40.0 27 35.896902856399244 39.0 36.0 40.0 29.0 40.0 28 35.806721322141975 39.0 35.0 40.0 29.0 40.0 29 35.76208601730757 39.0 35.0 40.0 29.0 40.0 30 35.723274123235086 39.0 35.0 40.0 29.0 40.0 31 35.490337692758146 39.0 35.0 40.0 28.0 40.0 32 35.54235799336326 39.0 35.0 40.0 28.0 40.0 33 35.54909232871364 39.0 35.0 40.0 28.0 40.0 34 35.07355716051793 39.0 35.0 40.0 26.0 40.0 35 35.17187195002928 39.0 35.0 40.0 27.0 40.0 36 34.95627561975405 39.0 35.0 40.0 26.0 40.0 37 34.85249528271195 39.0 35.0 40.0 26.0 40.0 38 34.89647992712603 39.0 35.0 40.0 26.0 40.0 39 34.33548051272041 39.0 35.0 40.0 22.0 40.0 40 34.58702583121869 39.0 34.0 40.0 25.0 40.0 41 34.4868566595094 39.0 35.0 40.0 24.0 40.0 42 34.19705250829592 38.0 34.0 40.0 22.0 40.0 43 34.23996356301646 38.0 34.0 40.0 23.0 40.0 44 34.10010410566725 38.0 34.0 40.0 23.0 40.0 45 33.991769145682866 38.0 34.0 40.0 22.0 40.0 46 33.771097664129094 38.0 34.0 40.0 18.0 40.0 47 33.66432428915349 38.0 34.0 40.0 16.0 40.0 48 33.42819962261696 38.0 33.0 40.0 14.0 40.0 49 33.18882165397879 38.0 33.0 39.0 10.0 40.0 50 32.825850738499575 38.0 33.0 39.0 4.0 40.0 51 32.45852039820418 38.0 33.0 39.0 4.0 40.0 52 32.32103585138916 38.0 32.0 39.0 4.0 40.0 53 31.88639469061097 37.0 32.0 39.0 4.0 40.0 54 31.586245038714296 37.0 31.0 39.0 4.0 40.0 55 31.05846183876635 37.0 31.0 39.0 4.0 40.0 56 30.085366647146856 36.0 29.0 39.0 4.0 40.0 57 29.668455982822564 36.0 28.0 39.0 4.0 40.0 58 29.38948532760752 36.0 27.0 39.0 4.0 40.0 59 28.94082243477129 35.0 24.0 39.0 4.0 40.0 60 28.517340100201704 35.0 22.0 39.0 4.0 40.0 61 27.95549482724966 35.0 4.0 39.0 4.0 40.0 62 27.728056477324483 35.0 4.0 39.0 4.0 40.0 63 27.38125447329039 35.0 4.0 39.0 4.0 40.0 64 27.371787364174637 35.0 4.0 39.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.050581721340151375 2 2 -0.19119228153721224 2 3 -0.07748933383301448 2 4 0.340046773175672 2 5 -0.17233273676011152 2 6 -0.15746437010623993 2 7 0.13859071296723613 2 8 0.09958911395298742 2 9 0.3976062008602739 2 10 -0.0258317586366843 2 11 -0.43306227087428795 2 12 0.08243168411375024 2 13 -0.7009166264635169 2 14 0.05432967360176377 2 15 0.08459058722738177 2 16 -0.3765075094864301 2 17 -1.0659049407520556 2 18 -0.025874733920112192 2 19 -0.8514862640219576 2 20 0.24909019206240401 2 21 0.25911841152640136 2 22 -0.416681338244949 2 23 0.19429503252381153 2 24 -0.8876407115661706 2 25 0.8421005510974595 2 26 -0.3542252614006145 2 27 1.0385383196094011 2 28 0.5488335483657707 2 29 0.9750691709999231 2 30 0.8342980289132456 2 31 0.5750557004048886 2 32 0.8630135795898966 2 33 -0.061149059325472876 2 34 -0.05442875318578899 2 35 0.6189728484334367 2 36 -2.310164739219786 2 37 -1.724689589457185 2 38 -2.2678035113402117 2 39 -5.379968985275781 2 40 -2.157399484826449 2 41 -3.037028426811961 2 42 -2.1911132559142885 2 43 -3.1684225953061294 2 44 -2.801884261955866 2 45 -3.811043415601837 2 46 -2.286330772810235 2 47 -2.4071867278307018 2 48 -2.102546712485271 2 49 -2.4236162742851377 2 50 -1.6608954409132188 2 51 0.08495403620901243 2 52 -0.10007492634255044 2 53 -0.9461493033785402 2 54 -0.662211243778092 2 55 -0.5311134979659151 2 56 1.3499963773366233 2 57 0.8913207243733474 2 58 1.0701134276403188 2 59 1.483930762743384 2 60 2.34927765814243 2 61 2.770089929634789 2 62 3.074254413337055 2 63 3.307649409197815 2 64 2.757551432932928 3 1 -0.6978309770725488 3 2 -0.33722402756895775 3 3 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0.19929698295496934 4 22 0.23778294746933426 4 23 0.2014378896666713 4 24 0.265037859862403 4 25 1.4804934082403136 4 26 1.7663104528851008 4 27 1.3385383196093983 4 28 1.4515121197943444 4 29 1.534890599571355 4 30 1.6932266003418164 4 31 0.8777342718334609 4 32 -0.32002213469581875 4 33 0.396886654960241 4 34 1.3294998182427804 4 35 -3.802455722995134 4 36 -0.15123616779121818 4 37 -0.14254673231432946 4 38 -0.14012493991164376 4 39 1.5066381575813637 4 40 -0.059185199112164355 4 41 -0.8316712839548188 4 42 -0.08307754162857606 4 43 -0.46395830959184536 4 44 0.262401452329847 4 45 0.5318137272553045 4 46 -0.20597362995309254 4 47 -0.6348652992592747 4 48 -1.6918324267709863 4 49 -0.027187702856565465 4 50 -0.31535972662750567 4 51 0.4313826076375804 4 52 0.9668893593717343 4 53 0.4663506966214612 4 54 1.346717327650481 4 55 1.3650203408488437E-4 4 56 0.3651749487651941 4 57 0.11810643865906201 4 58 -0.9718508580739673 4 59 -0.34196209439947367 4 60 0.43588480099957394 4 61 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1.3258639276875144 119 34 1.7594240606670226 119 35 1.083907913368499 119 36 1.8430820140269617 119 37 2.217301752534155 119 38 2.1278674843307783 119 39 2.011373006066215 119 40 2.188920861493898 119 41 2.5300711402876033 119 42 1.959536094735057 119 43 1.8930492661657325 119 44 1.724522664451058 119 45 2.1075713030128824 119 46 2.341374854895392 119 47 1.8092634886195142 119 48 2.3346827247441624 119 49 2.650842600173739 119 50 2.198844818827041 119 51 2.6321401833951583 119 52 2.4280636017959765 119 53 2.3205173632881255 119 54 1.3940658124989653 119 55 2.317371350518936 119 56 2.0062734336136785 119 57 1.6540912871439097 119 58 1.8510658085927005 119 59 2.596484875297495 119 60 3.2133469222116986 119 61 3.5060206655655257 119 62 3.5997955388781797 119 63 2.2860043875527936 119 64 2.6103652857467807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 71.0 5 13.0 6 17.0 7 20.0 8 60.0 9 39.0 10 56.0 11 57.0 12 63.0 13 86.0 14 108.0 15 114.0 16 132.0 17 130.0 18 148.0 19 178.0 20 192.0 21 224.0 22 252.0 23 284.0 24 262.0 25 329.0 26 387.0 27 464.0 28 635.0 29 688.0 30 850.0 31 1026.0 32 1226.0 33 1574.0 34 1894.0 35 2052.0 36 2702.0 37 3529.0 38 5105.0 39 5729.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.5282711952632 0.2309844492159542 0.1561585008783916 0.0845858546424621 8 99.0007160992123 0.5012694486036066 0.3222446455308899 0.17576980665321268 9 97.5762899342833 1.2037217776042684 0.5725811698874358 0.6474071182249984 10 40.26286680981196 38.297872340425535 7.459821719044831 13.979439130717678 11 23.033378879562758 39.64799271260329 23.38798880864077 13.930639599193182 12 51.2264948923157 17.883401652677467 18.394170082633874 12.495933372372958 13 24.52013794000911 17.057062918862645 34.05231309779426 24.370486043333983 14 33.45370551109376 26.381026742143277 21.24406272366452 18.921205023098445 15 33.43101820194718 22.89407704079971 21.708833968284978 21.96607078896812 16 23.561572507159596 28.911741733923456 25.741994272324913 21.784691486592035 17 31.672261512889875 24.616237004204283 19.499397060261384 24.21210442264446 18 26.159802199232217 28.90884247511224 26.18908191814692 18.742273407508623 19 26.927581495217645 25.09597241199818 27.913332032012494 20.063114060771685 20 19.11563740482853 28.75642610789354 20.980022125333505 31.14791436194443 21 21.59216604853927 29.829526969874422 29.208146268462485 19.37016071312382 22 20.961025440822432 37.44225388769601 19.900448955690024 21.69627171579153 23 27.83850608367493 37.40321426247641 20.180232936430475 14.57804671741818 24 23.366736074960958 40.64289432587194 21.941697032795417 14.048672566371682 25 26.406926406926406 25.222146274777852 28.71464375223774 19.656283566058004 26 26.45776389431212 22.416373812312898 31.638032018742678 19.487830274632305 27 23.032279100097817 22.852950766221063 30.74013694163678 23.374633192044342 28 27.230949742520043 27.419985659344242 26.98324750668144 18.36581709145427 29 30.45248721373424 23.477864286412352 28.045085839007065 18.024562660846335 30 25.58670143415906 31.945893089960887 29.194915254237287 13.272490221642762 31 25.554179162863477 30.45051506063372 30.727604642065458 13.267701134437345 32 18.369607715365568 30.519353577479475 37.86002867196664 13.251010035188324 33 18.721476072499673 32.6965706089451 34.86438909896988 13.717564219585343 34 16.821209350920725 34.74598406686692 35.16390231161029 13.268904270602064 35 15.610121959173027 38.955194678145176 34.41596556446879 11.018717798213004 36 15.124897959183672 35.813877551020404 34.331428571428575 14.729795918367348 37 14.76608664424929 35.558747673925104 37.24331559531194 12.431850086513663 38 14.793420794987272 36.10730370080282 37.115723516741724 11.983551987468182 39 13.792538731125742 35.95362090989486 36.63162032033016 13.62222003864924 40 14.589794652476249 36.31941497176064 38.50347686983774 10.58731350592537 41 12.932301361810522 37.79758988929166 38.614023056072625 10.656085692825187 42 13.062532705389849 37.706043956043956 37.09445316588174 12.136970172684459 43 14.236201935652629 37.0062777923097 37.80408056500131 10.95343970703636 44 13.350982122430304 35.97738340360166 38.458018760009146 12.213615713958886 45 14.137615278958728 38.05023219307999 36.791157041009875 11.020995486951403 46 13.354260676214766 37.195082074422864 37.528611601595706 11.92204564776666 47 15.32600876332483 37.03158720816167 36.57707147995553 11.065332548557976 48 15.461085676913013 37.47547416612165 36.749509483322434 10.313930673642904 49 14.955685646073846 37.54783006835203 36.972234032115644 10.524250253458483 50 12.98284666753961 38.35275631792589 36.81746759198638 11.84692942254812 51 13.149717052108207 38.60848516567989 37.061267214026365 11.180530568185535 52 15.270823105874653 38.48469972181313 34.91081656030109 11.333660612011128 53 12.778559507837297 39.93586177558166 35.92395039104683 11.361628325534213 54 14.566632856722181 39.194450806530774 35.71966102804044 10.519255308706606 55 13.489361702127658 39.276595744680854 33.92144026186579 13.312602291325696 56 14.263733385713351 40.594513193216784 33.54285340142735 11.598900019642507 57 14.5985401459854 39.772838859611795 34.35239435697686 11.276226637425943 58 16.028034322394706 39.12687495906203 31.889041723979826 12.956048994563437 59 14.49204165847907 39.70983166306413 33.34643348398507 12.451693194471737 60 14.932022932022932 39.0990990990991 32.17690417690417 13.791973791973792 61 15.006218498396281 39.08817176147149 32.4278326896642 13.477777050468024 62 14.608029340493811 39.21998821140873 31.478813281812823 14.693169166284628 63 14.55837098147057 40.57159693576901 31.126825116218164 13.743206966542266 64 17.75462052693669 39.13029230567571 29.430462708087564 13.684624459300041 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 2.5 5 46.0 6 88.0 7 94.0 8 100.0 9 106.5 10 99.0 11 85.0 12 142.5 13 287.0 14 374.0 15 407.0 16 566.5 17 693.0 18 729.5 19 766.0 20 923.0 21 1297.0 22 1514.0 23 1691.5 24 2013.5 25 2158.0 26 1736.0 27 1417.0 28 1520.0 29 1324.5 30 1009.5 31 890.0 32 875.5 33 861.0 34 868.5 35 885.0 36 894.0 37 932.0 38 889.5 39 809.0 40 825.0 41 798.5 42 756.0 43 757.5 44 759.0 45 738.5 46 753.0 47 788.0 48 911.5 49 1006.5 50 978.0 51 1065.0 52 1126.0 53 1100.0 54 955.5 55 722.5 56 634.0 57 604.0 58 574.0 59 502.0 60 398.0 61 366.0 62 326.5 63 222.5 64 158.0 65 244.5 66 208.0 67 85.0 68 78.0 69 71.0 70 58.5 71 33.5 72 21.0 73 20.5 74 14.5 75 9.0 76 8.5 77 5.5 78 3.0 79 4.0 80 2.5 81 0.0 82 0.5 83 1.0 84 0.5 85 0.5 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.052052833626130515 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08783915674409526 16 0.03253302101633158 17 0.17893161558982368 18 0.0 19 0.0 20 0.013013208406532629 21 0.0 22 0.0 23 0.0 24 0.006506604203266314 25 0.04879953152449736 26 0.01951981260979895 27 0.22122454291105473 28 0.1821849176914568 29 0.13338538616695947 30 0.18869152189472316 31 0.2017047303012558 32 0.14965189667512524 33 0.2017047303012558 34 0.35786323117964736 35 0.23423775131758734 36 0.36762313748454684 37 0.3481033248747478 38 0.3123170017567832 39 0.6734335350380636 40 0.3481033248747478 41 0.3806363458910794 42 0.5270349404645716 43 0.5010085236515063 44 0.45871559633027525 45 0.5205283362613052 46 0.5075151278547726 47 0.5075151278547726 48 0.5140217320580389 49 0.5237816383629383 50 0.6181273993102999 51 0.5433014509727373 52 0.5953542845988679 53 0.5823410761923352 54 0.5693278677858026 55 0.6116207951070336 56 0.6246340035135663 57 0.6083674930054005 58 0.6636736287331642 59 0.6636736287331642 60 0.6929533476478625 61 0.598607586700501 62 0.6506604203266315 63 0.6246340035135663 64 0.722233066562561 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 30738.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.240939553646946 #Duplication Level Percentage of deduplicated Percentage of total 1 82.34991511909048 52.07886004294359 2 8.63727558001955 10.924588457284143 3 3.63187406759607 6.890493851259027 4 1.7284839755131436 4.372438024594963 5 1.0185709141416739 3.2207690806168263 6 0.6327485981789186 2.4009369510052703 7 0.4887082668861567 2.1634458975860498 8 0.35495653068573485 1.7958227601015029 9 0.19548330675446265 1.1126293187585399 >10 0.8951077730335922 9.216604853926736 >50 0.04115438036936057 1.6949703949508752 >100 0.025721487730850354 4.128440366972478 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 428 1.3924132994989913 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 360 1.1711887565879369 No Hit AAAGTTGGGGGTCGTGTGGTGGGGAGAAGAGGACAGGGCGGATCGTGAGCCGAGCAGGCCACCG 215 0.699459951851129 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.5042618257531394 No Hit AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 111 0.3611165332812805 No Hit AAAGTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG 74 0.24074435552085366 No Hit AAAGTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.23423775131758734 No Hit AAAGTTGGGGTGATGTGGGTGTGGTACCCGAGTAGCGCGGGACTCGTGAAATCTCGTGTGAATC 70 0.22773114711432105 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.22447784501268786 No Hit AAAGTTGGGCATGCTGGCCCTCTCTGGGTATGTCCAGGGCTGCCGGGCTCTCATGATCACCGCC 66 0.2147179387077884 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.18869152189472316 No Hit AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC 57 0.18543821979309 No Hit AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.17893161558982368 No Hit AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.15941180298002472 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.1561585008783916 No Hit AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG 48 0.1561585008783916 No Hit AAAGTTGGGAATTCTCAAGCAAAGATACCGCCTGACTCGGAGCTAAAGTTGGCTGACCTGCTCA 47 0.1529051987767584 No Hit AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG 46 0.14965189667512524 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.14314529247185895 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.1301320840653263 No Hit AAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.12687878196369315 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.12687878196369315 No Hit AAAGTTGGGGATTCCAGTAGTATTTATGTTGAAAGAAGCAAATTTTAGTCCTTCCAACTGTGGA 38 0.12362547986206 No Hit AAAGTTGGGATGGCGAAAGAGATCGGTGCTGTCAAATACCTGGAGTGCTCAGCTCTCACACAGC 38 0.12362547986206 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.11711887565879367 No Hit AAAGTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.10410566725226103 No Hit AAAGTTGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTCC 31 0.1008523651506279 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0032533021016331576 0.0 0.0 0.0 0.0 8 0.0032533021016331576 0.0 0.0 0.0 0.0 9 0.0032533021016331576 0.0 0.0 0.0 0.0 10 0.04554622942286421 0.0 0.0 0.0 0.0 11 0.08783915674409526 0.0 0.0 0.0 0.0 12 0.09109245884572842 0.0 0.0 0.0 0.0 13 0.09109245884572842 0.0 0.0 0.0 0.0 14 0.09759906304899472 0.0 0.0 0.0 0.0 15 0.1073589693538942 0.0 0.0 0.0 0.0 16 0.12362547986205999 0.0 0.0 0.0 0.0 17 0.12687878196369315 0.0 0.0 0.0 0.0 18 0.14314529247185895 0.0 0.0 0.0 0.0 19 0.1463985945734921 0.0 0.0 0.0 0.0 20 0.15941180298002472 0.0 0.0 0.0 0.0 21 0.17567831348819052 0.0 0.0 0.0 0.0 22 0.22773114711432105 0.0 0.0 0.0 0.0 23 0.2797839807404516 0.0 0.0 0.0 0.0 24 0.3058103975535168 0.0 0.0 0.0 0.0 25 0.3220769080616826 0.0 0.0 0.0 0.0 26 0.3318368143665821 0.0 0.0 0.0 0.0 27 0.35786323117964736 0.0 0.0 0.0 0.0 28 0.38714295009434574 0.0 0.0 0.0 0.0 29 0.4066627627041447 0.0 0.0 0.0 0.0 30 0.4294358774155768 0.0 0.0 0.0 0.0 31 0.4391957837204763 0.0 0.0 0.0 0.0 32 0.4619688984319084 0.0 0.0 0.0 0.0 33 0.474982106838441 0.0 0.0 0.0 0.0 34 0.4912486173466068 0.0 0.0 0.0 0.0 35 0.5628212635825363 0.0 0.0 0.0 0.0 36 0.5725811698874358 0.0 0.0 0.0 0.0 37 0.5823410761923352 0.0 0.0 0.0 0.0 38 0.6051141909037673 0.0 0.0 0.0 0.0 39 0.6278873056151995 0.0 0.0 0.0 0.0 40 0.6409005140217321 0.0 0.0 0.0 0.0 41 0.6701802329364305 0.0 0.0 0.0 0.0 42 0.7027132539527621 0.0 0.0 0.0 0.0 43 0.7189797644609278 0.0 0.0 0.0 0.0 44 0.7612726917821588 0.0 0.0 0.0 0.0 45 0.8165788275099226 0.0 0.0 0.0 0.0 46 0.8523651506278873 0.0 0.0 0.0 0.0 47 0.8848981716442189 0.0 0.0 0.0 0.0 48 0.9044179842540179 0.0 0.0 0.0 0.0 49 0.9467109115752489 0.0 0.0 0.0 0.0 50 0.9727373283883142 0.0 0.0 0.0 0.0 51 1.0085236515062788 0.0 0.0 0.0 0.0 52 1.0247901620144446 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACAG 40 6.4210326E-10 58.36903 30 GAGGACA 40 6.530172E-10 58.273026 29 GGGCGGA 40 6.530172E-10 58.273026 36 CTCCTGT 15 0.0045580077 58.27302 37 AGCTGCT 15 0.0045580077 58.27302 39 GTCCAGG 15 0.0045878333 58.177338 32 ACTGAGT 25 8.39924E-6 58.081966 45 GATCGTG 40 6.712071E-10 58.081966 41 GAAGAGG 40 6.712071E-10 58.081966 26 TCGTGAG 40 6.712071E-10 58.081966 43 GTGAGCC 40 6.712071E-10 58.081966 45 GACACGC 25 8.39924E-6 58.081966 56 AGCAGGC 40 6.712071E-10 58.081966 53 AGGCCAC 40 6.712071E-10 58.081966 56 GGCCACC 40 6.712071E-10 58.081966 57 CGAGCAG 40 6.712071E-10 58.081966 51 CCGAGCA 40 6.712071E-10 58.081966 50 CAGGCCA 40 6.712071E-10 58.081966 55 ATCGTGA 40 6.712071E-10 58.081966 42 CGTGAGC 40 6.712071E-10 58.081966 44 >>END_MODULE