FastQCFastQC Report
Fri 17 Jun 2016
SRR1526560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26932
Sequences flagged as poor quality0
Sequence length56
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5752.135006683499183No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5091.889945046784494No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA2360.8762810040100995No Hit
AAAGTTGGGGGTCGTGTGGTGGGGAGAAGAGGACAGGGCGGATCGTGAGCCGAGCA2100.7797415713649191No Hit
AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1610.5978018713797713No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1070.397296895885935No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA760.28219218773206595No Hit
AAAGTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.2747660775285905No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA700.25991385712163967No Hit
AAAGTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG700.25991385712163967No Hit
AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.24506163671468884No Hit
AAAGTTGGGCATGCTGGCCCTCTCTGGGTATGTCCAGGGCTGCCGGGCTCTCATGA580.21535719590078717No Hit
AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA560.20793108569731175No Hit
AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.20793108569731175No Hit
AAAGTTGGGGTGATGTGGGTGTGGTACCCGAGTAGCGCGGGACTCGTGAAATCTCG540.2005049754938363No Hit
AAAGTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.19307886529036092No Hit
AAAGTTGGGGATTCCAGTAGTATTTATGTTGAAAGAAGCAAATTTTAGTCCTTCCA480.17822664488341008No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.17451358978167236No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA470.17451358978167236No Hit
AAAGTTGGGAATTCTCAAGCAAAGATACCGCCTGACTCGGAGCTAAAGTTGGCTGA470.17451358978167236No Hit
AAAGTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.16337442447645925No Hit
AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA440.16337442447645925No Hit
AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.16337442447645925No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA420.1559483142729838No Hit
AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1485222040695084No Hit
AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA400.1485222040695084No Hit
AAAGTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG390.1448091489677707No Hit
AAAGTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.14109609386603297No Hit
AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC380.14109609386603297No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA360.13366998366255756No Hit
AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.12995692856081983No Hit
AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG350.12995692856081983No Hit
AAAGTTGGGATGGCGAAAGAGATCGGTGCTGTCAAATACCTGGAGTGCTCAGCTCT340.12624387345908214No Hit
AAAGTTGGGCAGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAA330.12253081835734442No Hit
AAAGTTGGGGTCTGCCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCAGTCCACA320.11881776325560671No Hit
AAAGTTGGGACGCGCGAGCCACATCGTCTGTGGTCGTCCGGTATCCGAAGTCCCCG310.115104708153869No Hit
AAAGTTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA300.1113916530521313No Hit
AAAGTTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1113916530521313No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAA300.1113916530521313No Hit
AAAGTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1113916530521313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAT203.7945554E-450.8136937
CTCTACG505.456968E-1250.81368637
ACTCTAC505.456968E-1250.71726636
AACTCTA505.456968E-1250.71726635
GGGCGGA203.830425E-450.71726636
TCAAACT505.456968E-1250.5255232
GTACTGA505.456968E-1250.33521743
TCTACGT505.456968E-1250.33521738
TACGTAC505.456968E-1250.33521740
TACTGAG505.456968E-1250.33521744
CTACGTA505.456968E-1250.33521739
ATCAAAC451.1823431E-1050.33521731
TGAGTCG451.1823431E-1050.33521747
AACACCC150.00812373950.33521748
GGTAAAG150.00812373950.33521738
GTTTGAT203.9766068E-450.33521348
GGATCGT203.9766068E-450.33521340
GAGCCGA203.9766068E-450.33521347
GGACAGG203.9766068E-450.33521331
CGGATCG203.9766068E-450.33521339