##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526560_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26932 Sequences flagged as poor quality 0 Sequence length 56 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.01006237932571 39.0 38.0 40.0 35.0 40.0 2 37.78731620377246 39.0 38.0 40.0 34.0 40.0 3 37.57767711272835 39.0 38.0 40.0 33.0 40.0 4 37.548009802465465 39.0 38.0 40.0 33.0 40.0 5 36.65145551759988 39.0 36.0 40.0 31.0 40.0 6 38.01080499034606 39.0 38.0 40.0 35.0 40.0 7 37.68190256943413 39.0 38.0 40.0 35.0 40.0 8 37.91883261547601 39.0 38.0 40.0 35.0 40.0 9 37.89254418535571 39.0 38.0 40.0 35.0 40.0 10 37.16300311896629 39.0 37.0 40.0 33.0 40.0 11 37.54218030595574 39.0 38.0 40.0 33.0 40.0 12 37.48221446606268 39.0 38.0 40.0 33.0 40.0 13 37.35708450913412 39.0 37.0 40.0 33.0 40.0 14 37.58157582058518 39.0 38.0 40.0 33.0 40.0 15 37.56731768899451 39.0 38.0 40.0 33.0 40.0 16 37.49643546710233 39.0 38.0 40.0 33.0 40.0 17 37.452472894697756 39.0 38.0 40.0 33.0 40.0 18 37.3617629585623 39.0 38.0 40.0 33.0 40.0 19 37.28998960344572 39.0 37.0 40.0 33.0 40.0 20 37.31007723154612 39.0 38.0 40.0 33.0 40.0 21 37.23310559928709 39.0 38.0 40.0 33.0 40.0 22 37.131182236744394 39.0 37.0 40.0 33.0 40.0 23 36.92525620080202 39.0 36.0 40.0 32.0 40.0 24 36.7929600475271 39.0 36.0 40.0 32.0 40.0 25 36.73228872716471 39.0 36.0 40.0 32.0 40.0 26 36.36722114956186 39.0 36.0 40.0 31.0 40.0 27 36.39206148819248 39.0 36.0 40.0 31.0 40.0 28 36.16745878508837 38.0 36.0 40.0 31.0 40.0 29 36.22241200059409 39.0 36.0 40.0 31.0 40.0 30 35.98258577157285 38.0 35.0 40.0 30.0 40.0 31 35.589261844645776 38.0 35.0 40.0 29.0 40.0 32 35.24168275657211 38.0 35.0 40.0 28.0 40.0 33 35.3040620822813 38.0 35.0 40.0 28.0 40.0 34 35.50033417495916 38.0 35.0 40.0 29.0 40.0 35 35.280484182385266 38.0 35.0 40.0 28.0 40.0 36 35.11506757760285 39.0 35.0 40.0 27.0 40.0 37 34.72218921728798 39.0 35.0 40.0 25.0 40.0 38 34.802688251893656 39.0 35.0 40.0 26.0 40.0 39 34.752153571959006 39.0 35.0 40.0 26.0 40.0 40 34.34056141393138 38.0 35.0 40.0 23.0 40.0 41 34.198425664636865 38.0 34.0 40.0 23.0 40.0 42 34.09702212980841 38.0 34.0 40.0 23.0 40.0 43 34.03835585920095 38.0 34.0 40.0 22.0 40.0 44 33.54919798009802 38.0 33.0 40.0 17.0 40.0 45 33.64042774394772 38.0 33.0 40.0 18.0 40.0 46 33.243427892469924 38.0 33.0 39.0 7.0 40.0 47 33.13296450319323 38.0 33.0 39.0 4.0 40.0 48 32.580536165156694 38.0 32.0 39.0 4.0 40.0 49 32.57177335511659 38.0 32.0 39.0 4.0 40.0 50 32.34334620525769 38.0 32.0 39.0 4.0 40.0 51 31.706074558146444 38.0 32.0 39.0 4.0 40.0 52 31.518899450467845 37.0 32.0 39.0 4.0 40.0 53 30.993427892469924 37.0 31.0 39.0 4.0 40.0 54 30.60559928709342 37.0 30.0 39.0 4.0 40.0 55 29.918684093271946 36.0 29.0 39.0 4.0 40.0 56 17.67384523986336 21.0 4.0 25.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -1.6336065612447257 1 2 -0.7590008100929211 1 3 -1.4964230433802967 1 4 -2.6963538029566436 1 5 -1.255521656938349 1 6 -1.4440600519474174 1 7 -1.6449954986100437 1 8 -2.725494234953871 1 9 -2.578439945340399 1 10 -0.8441459663839836 1 11 -0.648741687591837 1 12 -1.6503090552978534 1 13 -0.6372345941279605 1 14 -1.8493477858840066 1 15 -0.9016053205008703 1 16 -1.3252439338939297 1 17 -0.9751791959811271 1 18 -2.1555674970273486 1 19 -1.157941691248844 1 20 -2.673169899191727 1 21 -0.9165963651178899 1 22 -1.5555519007652805 1 23 -1.6105681511342738 1 24 -2.0938385227809704 1 25 -1.6048240965924236 1 26 -0.026592447686120124 1 27 -1.1337169910087894 1 28 -0.4223356289771161 1 29 -0.7072216828516602 1 30 0.11708846541282014 1 31 -0.030715193906985405 1 32 0.5562748494426302 1 33 0.5821429793887773 1 34 0.5765813128519639 1 35 0.37009233432205235 1 36 -0.6258218350801812 1 37 -1.6979905276193037 1 38 -0.5740142908321957 1 39 -0.9225594794279033 1 40 -0.40296954154840847 1 41 -1.668951388574179 1 42 -3.316400025421512 1 43 -1.2107230494308823 1 44 -0.8373312215693574 1 45 -0.47024805024771865 1 46 -2.024161458509706 1 47 -2.3466117931773915 1 48 -1.8072660108766812 1 49 -1.3086966074211759 1 50 -3.1192005171180917 1 51 -2.2684949617621015 1 52 -1.3886877019346358 1 53 -1.969433208376298 1 54 -1.583212411600151 1 55 -1.3143257864849147 1 56 2.994234746658101 2 1 0.468404933008145 2 2 1.292723327838111 2 3 0.4489792554702774 2 4 0.9616921740548463 2 5 1.468616274096135 2 6 0.6292158101215506 2 7 0.563337834723292 2 8 0.3089885236668195 2 9 -0.006600864880631718 2 10 0.4791298956849843 2 11 -0.3168451358677018 2 12 0.24767945044927586 2 13 -0.9317748240130186 2 14 -0.7918765215161869 2 15 -1.0926972745238643 2 16 -0.8784048534341622 2 17 -1.2337998856363015 2 18 -1.6670617499009097 2 19 -1.0832290475706827 2 20 -1.1674227727549464 2 21 -0.9051021122443288 2 22 -1.200666843294016 2 23 -0.8404532086055383 2 24 -0.25763162622924796 2 25 -0.9094217977418495 2 26 -1.7809027925137073 2 27 -0.4253836576754537 2 28 -2.186703445069071 2 29 -1.1799228322769437 2 30 -1.0423942932078702 2 31 -1.3094508260908881 2 32 -0.9379780241205893 2 33 -0.3963052964732938 2 34 0.06652384158759617 2 35 -0.7089306541836962 2 36 0.19170690055199913 2 37 -0.4221284586537877 2 38 -0.05102578508506639 2 39 0.3547393711468132 2 40 1.0524902285665334 2 41 1.1959911401614534 2 42 1.4436574458428524 2 43 0.963127525281763 2 44 0.2819216519938621 2 45 1.7696944784879136 2 46 2.2157810702259297 2 47 2.0485031493513475 2 48 1.3263546787784932 2 49 2.0964758063719273 2 50 2.2184431610428277 2 51 1.797596992260889 2 52 2.3756801141573156 2 53 2.5276932284053117 2 54 3.27167264587111 2 55 1.109524788227727 2 56 1.3548669305661498 3 1 0.12102306838490051 3 2 0.6669251158330027 3 3 -0.11679341375066343 3 4 0.23883138222853972 3 5 -0.6629290643457537 3 6 0.06982883694146835 3 7 0.5263007976862539 3 8 0.01524650578686959 3 9 0.301189684289227 3 10 0.14196514472713062 3 11 -0.3385565024066537 3 12 -0.5160497960385939 3 13 -0.16038274227611282 3 14 -0.1826811192173352 3 15 0.20487616098061068 3 16 0.05901532536532983 3 17 0.9322282114262777 3 18 -1.2481600896199367 3 19 0.11983608652893452 3 20 -0.11761434363616985 3 21 -0.6943741428956685 3 22 -0.3564778266912043 3 23 0.2042466636805358 3 24 -0.9549496338920846 3 25 0.6729536811853549 3 26 -1.1423331884268606 3 27 1.426468194176401 3 28 0.33692363028214345 3 29 0.6770375764075993 3 30 -0.9708744975501418 3 31 -0.22515963835142827 3 32 -0.7770584838906984 3 33 -0.8484125761667798 3 34 -1.3076779464072956 3 35 -1.1623150730853595 3 36 -0.6026736869320359 3 37 -0.6609534905822656 3 38 0.18524496842706384 3 39 -0.5660779979464223 3 40 0.009067495488629618 3 41 -0.5856180552408503 3 42 -1.9228815069029963 3 43 -1.4097971235049513 3 44 -1.027146036384174 3 45 -1.9008036058032758 3 46 -2.195457754806 3 47 -3.943834015399613 3 48 -2.7655993442100133 3 49 -2.373511422235989 3 50 -3.3460523689699464 3 51 -3.3518282950954372 3 52 -2.5923914056383417 3 53 -1.9601739491170385 3 54 -2.2174716708594104 3 55 -2.3004368975960254 3 56 -1.4178022903789333 4 1 0.05461182955986743 4 2 0.3616888450794917 4 3 0.1041516692633806 4 4 -0.07279058456584409 4 5 -0.8762113121107618 4 6 -0.0949221209129334 4 7 0.3219585243784664 4 8 -0.24273561426421253 4 9 -0.3514284510875285 4 10 -1.0381114836253644 4 11 -1.0409830669021787 4 12 -1.545423997826589 4 13 -1.0697058584957801 4 14 -1.4125661766885997 4 15 -0.9547662400411028 4 16 -0.671508301710027 4 17 -0.16483436839492072 4 18 -0.5980962326595289 4 19 -0.25564284067412757 4 20 -0.37431932447908167 4 21 -0.5257917674167416 4 22 -0.5799771881216031 4 23 -0.1507980361917447 4 24 -0.25763162622924796 4 25 -0.32321490119012 4 26 -0.26366141320335856 4 27 0.33323703197972065 4 28 -0.22118620368976138 4 29 -0.6281986943459117 4 30 -0.14584256906994142 4 31 -0.8611749640219273 4 32 -0.4552194034309309 4 33 -0.4652708137146675 4 34 -0.5196830549641334 4 35 -0.6399651369423154 4 36 0.7434310384830383 4 37 -0.7669560448606845 4 38 -0.2579223368092016 4 39 -0.5418123529911227 4 40 -0.49923390936450573 4 41 0.9201290711959302 4 42 0.16779537687733637 4 43 0.06657580114383421 4 44 0.9370940657869653 4 45 -0.195822762891396 4 46 0.49164313919144575 4 47 1.2209169424547923 4 48 1.7401477822267637 4 49 0.7171654615443401 4 50 1.3908569541462725 4 51 1.3148383715712306 4 52 1.6170594245021412 4 53 1.2518311594397957 4 54 1.547534714836626 4 55 1.0750420296070367 4 56 -0.989960655640747 5 1 -0.2586065612447257 5 2 1.115999189907079 5 3 0.37857695661970325 5 4 0.6786461970433564 5 5 1.119478343061651 5 6 -0.15239338528075308 5 7 -0.22832883194337228 5 8 -0.2671609016205352 5 9 -0.3701066120070706 5 10 -0.09414596638398365 5 11 -0.8154083542585013 5 12 -0.19197572196451773 5 13 -0.09556792746129616 5 14 -0.8076811192173352 5 15 -0.859938653834206 5 16 -0.2835772672272654 5 17 -1.4751791959811271 5 18 -1.072234163694013 5 19 -0.532941691248844 5 20 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30 1.1772736505980035 7 31 0.885951472759686 7 32 0.4081267012944849 7 33 1.2997355719813726 7 34 1.544173905444552 7 35 1.9117590009887167 7 36 2.767696683438338 7 37 3.190898361269582 7 38 2.8889486721307662 7 39 3.137625705757287 7 40 2.8979563843775153 7 41 3.59956712994434 7 42 3.4474888634673775 7 43 2.9605732468654153 7 44 3.454335445097307 7 45 3.2103075053078314 7 46 3.5452829859347403 7 47 2.908017836452238 7 48 3.234400655789983 7 49 3.5153774666529003 7 50 3.8391328162152405 7 51 3.055579112311971 7 52 3.1113122980653607 7 53 3.484270495327408 7 54 3.745491292103555 7 55 4.403266806107677 7 56 3.100716228139582 8 1 0.34324529060712194 8 2 0.07433252324041462 8 3 0.32765103069377943 8 4 0.42401656741372307 8 5 0.44818204676535345 8 6 0.44019920731184214 8 7 0.8966711680566277 8 8 0.9411724317127934 8 9 0.8567452398447841 8 10 1.1790021817641616 8 11 0.5873694235192772 8 12 0.18765390766510848 8 13 0.6544320725387038 8 14 0.5580596215234053 8 15 0.20487616098061068 8 16 1.0590153253653298 8 17 0.9322282114262777 8 18 0.8259139844541323 8 19 0.9346509013437512 8 20 1.0305338045119825 8 21 0.19451474599321728 8 22 0.6064851362717576 8 23 0.09313555256942863 8 24 0.6746799957375487 8 25 0.8951759034075764 8 26 0.5613705152768418 8 27 1.5375793052875082 8 28 1.3369236302821434 8 29 1.7881486875187136 8 30 1.362458835783194 8 31 1.0340996209078313 8 32 0.445163738331523 8 33 1.040476312722113 8 34 0.9145442758149258 8 35 1.9487960380257547 8 36 2.7306596464012998 8 37 2.7834909538621773 8 38 2.703763486945583 8 39 2.7672553353869134 8 40 3.268326754747882 8 41 2.3032708336480425 8 42 2.817859233837744 8 43 1.6272399135320796 8 44 1.8247058154676807 8 45 1.8399371349374647 8 46 2.471208911860664 8 47 3.6117215401559406 8 48 3.3455117669010974 8 49 3.959821911097343 8 50 3.653947631030057 8 51 3.2778013345341925 8 52 3.5557567425098107 8 53 2.1509371619940723 8 54 1.3010468476591086 8 55 1.5143779172187877 8 56 1.8784940059173607 9 1 0.2413934387552743 9 2 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49 -0.21254276126732918 120 50 0.1468251239075471 120 51 -0.492853936121076 120 52 -0.4015082147551503 120 53 -1.270715259658349 120 54 -2.278725232112972 120 55 -3.3559924531515826 120 56 -1.5987139712906142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 44.0 5 13.0 6 3.0 7 9.0 8 31.0 9 25.0 10 36.0 11 34.0 12 49.0 13 43.0 14 68.0 15 76.0 16 99.0 17 67.0 18 121.0 19 130.0 20 157.0 21 166.0 22 168.0 23 192.0 24 275.0 25 242.0 26 301.0 27 274.0 28 358.0 29 456.0 30 563.0 31 723.0 32 878.0 33 1073.0 34 1444.0 35 1815.0 36 2405.0 37 4004.0 38 5345.0 39 5245.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.42044061373852 0.26748894750529406 0.21176208344169112 0.10030835531448527 8 99.34278924699242 0.3824446754789841 0.1485222040695084 0.12624387345908214 9 97.83157582058519 1.1361948611317392 0.4381405020050497 0.5940888162780336 10 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21.918164265557703 23 27.212980840635677 36.707262735779 20.54433387791475 15.535422545670578 24 22.991237189959897 40.917867221149564 21.977573147185502 14.113322441705034 25 26.872933922668352 24.937785536530104 28.377223934925528 19.812056605876016 26 26.31324764098373 23.010624860688015 31.369343933427444 19.306783564900808 27 23.393421297156664 21.739453633153687 30.983088645233224 23.88403642445642 28 26.947665774606005 27.349093071662207 27.326791555159087 18.3764495985727 29 30.865620584516996 22.573808284375694 28.742470439503236 17.818100691604073 30 25.822294984372675 31.50022324750707 29.137520464354814 13.539961303765441 31 25.953488372093027 29.949767441860466 30.600930232558138 13.495813953488373 32 18.92988792493577 30.327288974941357 37.18955951893361 13.553263581189261 33 19.07723244209429 32.38996052729575 34.393386460117675 14.139420570492293 34 17.21543504171633 34.55750893921335 35.03799165673421 13.189064362336115 35 15.284867906248836 38.77109960129672 35.23121064202407 10.712821850430377 36 15.109040074557317 35.62348555452004 34.426840633737186 14.84063373718546 37 14.894657637321293 34.85703536493604 37.2084273890143 13.039879608728366 38 15.115671641791046 35.37686567164179 37.42164179104478 12.085820895522389 39 13.74883373763762 35.39466318342974 36.88001492815824 13.976488150774399 40 14.851374088614694 35.37109740138343 38.72125630959058 11.056272200411293 41 13.317896623842246 36.96967433522558 38.65401852405139 11.05841051688079 42 12.832965965554601 37.34075540777824 37.606007397168156 12.220271229499009 43 14.131937955522334 36.43057372453747 38.22836852924687 11.209119790693329 44 13.75313166062147 35.141906293235614 38.697977040720936 12.40698500542198 45 14.290521592821086 37.124696204898115 37.36399326977005 11.22078893251075 46 13.200209408421209 36.63151596739212 38.02632563009498 12.14194899409169 47 15.875864647597682 36.26472237801458 36.668536174985974 11.190876799401757 48 16.12456488378186 36.8491971403975 36.27278511808961 10.753452857731032 49 15.396706586826348 37.12200598802396 36.93488023952096 10.546407185628743 50 13.676844783715012 38.025744649004636 36.8021254303248 11.495285136955545 51 13.667826542447711 38.021476409623226 36.872825232910536 11.43787181501852 52 15.474452554744525 37.83642148605652 34.92045667228149 11.768669286917461 53 12.677374667715002 39.14410872739526 36.31734621288704 11.861170392002695 54 15.72915886450453 38.06456445210097 35.37562729383566 10.830649389558834 55 14.324243332334433 38.81854959544501 33.832783937668566 13.024423134551993 56 14.989886882912579 39.73331335680575 33.343321597123385 11.933478163158288 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 1.0 3 1.0 4 2.5 5 4.0 6 54.0 7 104.0 8 97.0 9 90.0 10 81.0 11 72.0 12 70.5 13 147.0 14 225.0 15 285.5 16 346.0 17 398.0 18 450.0 19 543.0 20 636.0 21 725.0 22 1016.0 23 1218.0 24 1419.5 25 1621.0 26 1923.0 27 2225.0 28 2297.0 29 1880.0 30 1391.0 31 1458.0 32 1525.0 33 1203.5 34 882.0 35 764.0 36 646.0 37 592.0 38 594.5 39 651.0 40 646.0 41 641.0 42 661.0 43 681.0 44 716.5 45 752.0 46 801.0 47 984.0 48 1118.0 49 1242.5 50 1367.0 51 1293.5 52 1220.0 53 1084.0 54 896.0 55 844.0 56 737.0 57 630.0 58 546.0 59 462.0 60 459.5 61 457.0 62 488.5 63 371.5 64 223.0 65 175.5 66 128.0 67 98.0 68 68.0 69 59.0 70 50.0 71 49.5 72 32.5 73 16.0 74 14.0 75 12.0 76 10.5 77 9.0 78 7.0 79 3.5 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05569582652606565 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.03341749591563939 26 0.05198277142432794 27 0.10025248774691815 28 0.10396554284865588 29 0.14109609386603297 30 0.20793108569731175 31 0.21164414079904945 32 0.2784791326303282 33 0.2896182979355414 34 0.3118966285459676 35 0.3527402346650824 36 0.397296895885935 37 1.306995395811674 38 0.49012327342937767 39 0.5086885489380663 40 0.6943413040249518 41 0.5792365958710828 42 0.6126540917867221 43 0.6572107530075746 44 0.7017674142284271 45 0.6943413040249518 46 0.7054804693301648 47 0.6943413040249518 48 0.7983068468736075 49 0.7871676815683946 50 0.7723154611614437 51 0.7611762958562305 52 0.805732957077083 53 0.8280112876875092 54 0.8540026733996733 55 0.8762810040100995 56 0.8688548938066241 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 26932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.643695232437246 #Duplication Level Percentage of deduplicated Percentage of total 1 74.74255889406122 39.34724491311451 2 12.23021582733813 12.876875092826378 3 5.106503032867823 8.064755680974306 4 2.489772887572295 5.242833803653646 5 1.4317957398786854 3.7687509282637754 6 0.9380730709550008 2.963017971186692 7 0.655945831570038 2.4171988712312493 8 0.4866694879390605 2.049606416159216 9 0.31739314430808296 1.5037873162037725 >10 1.488221187755678 12.743205109163819 >50 0.07053180984624066 2.3466508242982327 >100 0.028212723938496262 2.651121342640725 >500 0.014106361969248131 4.024951730283678 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAGTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 575 2.135006683499183 No Hit AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 509 1.889945046784494 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 236 0.8762810040100995 No Hit AAAGTTGGGGGTCGTGTGGTGGGGAGAAGAGGACAGGGCGGATCGTGAGCCGAGCA 210 0.7797415713649191 No Hit AAAGTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 161 0.5978018713797713 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.397296895885935 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.28219218773206595 No Hit AAAGTTGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74 0.2747660775285905 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 70 0.25991385712163967 No Hit AAAGTTGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 70 0.25991385712163967 No Hit AAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.24506163671468884 No Hit AAAGTTGGGCATGCTGGCCCTCTCTGGGTATGTCCAGGGCTGCCGGGCTCTCATGA 58 0.21535719590078717 No Hit AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA 56 0.20793108569731175 No Hit AAAGTTGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.20793108569731175 No Hit AAAGTTGGGGTGATGTGGGTGTGGTACCCGAGTAGCGCGGGACTCGTGAAATCTCG 54 0.2005049754938363 No Hit AAAGTTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52 0.19307886529036092 No Hit AAAGTTGGGGATTCCAGTAGTATTTATGTTGAAAGAAGCAAATTTTAGTCCTTCCA 48 0.17822664488341008 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.17451358978167236 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.17451358978167236 No Hit AAAGTTGGGAATTCTCAAGCAAAGATACCGCCTGACTCGGAGCTAAAGTTGGCTGA 47 0.17451358978167236 No Hit AAAGTTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.16337442447645925 No Hit AAAGTTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA 44 0.16337442447645925 No Hit AAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 44 0.16337442447645925 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 42 0.1559483142729838 No Hit AAAGTTGGGGTGCAGTGCTAAAGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.1485222040695084 No Hit AAAGTTGGGAGTGCAGTGCTAAAGTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.1485222040695084 No Hit AAAGTTGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 39 0.1448091489677707 No Hit AAAGTTGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.14109609386603297 No Hit AAAGTTGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 38 0.14109609386603297 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.13366998366255756 No Hit AAAGTTGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.12995692856081983 No Hit AAAGTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 35 0.12995692856081983 No Hit AAAGTTGGGATGGCGAAAGAGATCGGTGCTGTCAAATACCTGGAGTGCTCAGCTCT 34 0.12624387345908214 No Hit AAAGTTGGGCAGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAAAA 33 0.12253081835734442 No Hit AAAGTTGGGGTCTGCCCCCAGCCCAGACACCGAAGCTACTCTCCTTCCAGTCCACA 32 0.11881776325560671 No Hit AAAGTTGGGACGCGCGAGCCACATCGTCTGTGGTCGTCCGGTATCCGAAGTCCCCG 31 0.115104708153869 No Hit AAAGTTGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.1113916530521313 No Hit AAAGTTGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.1113916530521313 No Hit AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.1113916530521313 No Hit AAAGTTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30 0.1113916530521313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0037130551017377097 0.0 0.0 0.0 0.0 8 0.007426110203475419 0.0 0.0 0.0 0.0 9 0.011139165305213129 0.0 0.0 0.0 0.0 10 0.0779741571364919 0.0 0.0 0.0 0.0 11 0.10767859795039358 0.0 0.0 0.0 0.0 12 0.10767859795039358 0.0 0.0 0.0 0.0 13 0.10767859795039358 0.0 0.0 0.0 0.0 14 0.12253081835734442 0.0 0.0 0.0 0.0 15 0.13366998366255756 0.0 0.0 0.0 0.0 16 0.1448091489677707 0.0 0.0 0.0 0.0 17 0.1485222040695084 0.0 0.0 0.0 0.0 18 0.15966136937472153 0.0 0.0 0.0 0.0 19 0.17080053467993464 0.0 0.0 0.0 0.0 20 0.20050497549383633 0.0 0.0 0.0 0.0 21 0.2190702510025249 0.0 0.0 0.0 0.0 22 0.2673399673251151 0.0 0.0 0.0 0.0 23 0.3007574632407545 0.0 0.0 0.0 0.0 24 0.31932273874944306 0.0 0.0 0.0 0.0 25 0.3341749591563939 0.0 0.0 0.0 0.0 26 0.36387939997029556 0.0 0.0 0.0 0.0 27 0.36759245507203325 0.0 0.0 0.0 0.0 28 0.38987078568245953 0.0 0.0 0.0 0.0 29 0.40100995098767267 0.0 0.0 0.0 0.0 30 0.4381405020050498 0.0 0.0 0.0 0.0 31 0.4641318877172137 0.0 0.0 0.0 0.0 32 0.47527105302242684 0.0 0.0 0.0 0.0 33 0.4975493836328531 0.0 0.0 0.0 0.0 34 0.512401604039804 0.0 0.0 0.0 0.0 35 0.575523540769345 0.0 0.0 0.0 0.0 36 0.5792365958710828 0.0 0.0 0.0 0.0 37 0.601514926481509 0.0 0.0 0.0 0.0 38 0.6089410366849844 0.0 0.0 0.0 0.0 39 0.6275063121936729 0.0 0.0 0.0 0.0 40 0.6460715877023615 0.0 0.0 0.0 0.0 41 0.6609238081093124 0.0 0.0 0.0 0.0 42 0.6720629734145255 0.0 0.0 0.0 0.0 43 0.6943413040249518 0.0 0.0 0.0 0.0 44 0.7277587999405911 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGGAT 20 3.7945554E-4 50.81369 37 CTCTACG 50 5.456968E-12 50.813686 37 ACTCTAC 50 5.456968E-12 50.717266 36 AACTCTA 50 5.456968E-12 50.717266 35 GGGCGGA 20 3.830425E-4 50.717266 36 TCAAACT 50 5.456968E-12 50.52552 32 GTACTGA 50 5.456968E-12 50.335217 43 TCTACGT 50 5.456968E-12 50.335217 38 TACGTAC 50 5.456968E-12 50.335217 40 TACTGAG 50 5.456968E-12 50.335217 44 CTACGTA 50 5.456968E-12 50.335217 39 ATCAAAC 45 1.1823431E-10 50.335217 31 TGAGTCG 45 1.1823431E-10 50.335217 47 AACACCC 15 0.008123739 50.335217 48 GGTAAAG 15 0.008123739 50.335217 38 GTTTGAT 20 3.9766068E-4 50.335213 48 GGATCGT 20 3.9766068E-4 50.335213 40 GAGCCGA 20 3.9766068E-4 50.335213 47 GGACAGG 20 3.9766068E-4 50.335213 31 CGGATCG 20 3.9766068E-4 50.335213 39 >>END_MODULE