FastQCFastQC Report
Fri 17 Jun 2016
SRR1526559_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526559_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143424
Sequences flagged as poor quality0
Sequence length64
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA97630.8538389958580543No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76680.6706173737826038No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68590.5998649669763797No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63880.5586728982424717No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA57730.5048870760102989No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49750.4350966920407478No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46180.40387467815963285No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46160.4036997649166014No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46100.40317502518750703No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40030.35008885592746No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38410.335920883241912No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36790.32175291055636407No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34480.3015504309862308No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33500.29297968207768943No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33480.292804768834658No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30990.27102807007724167No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30620.26779217508115977No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29570.2586092298220083No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29100.25449876861076903No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28670.25073813388559274No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28440.248726631590731No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26970.23587050822791894No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26960.23578305160640323No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25440.22248964513601255No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22070.19301676368521214No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21770.1903930650397403No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21490.1879442796372999No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20200.17666237546177097No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19840.17351393708720475No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19020.16634249412291502No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18960.16581775439382065No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18290.15995816075226688No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17780.15549787305496474No Hit
ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17220.15060030225008397No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15870.13879365834546065No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15720.13748180902272472No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15420.13485811037725287No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14820.1296107130863092No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14770.12917342997873055No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14710.12864869024963618No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14530.1270744710623531No Hit
ATGCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG14250.12462568565991268No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13270.11605493675137131No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13270.11605493675137131No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12480.10914586365162879No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12410.1085336673010187No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC12270.1073092745997985Illumina Single End Adapter 2 (96% over 31bp)
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12270.1073092745997985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG201.9790525E-458.18448353
TCCATAT150.004642377658.14100346
CGACACT150.004674775558.0389633
CAACGAT258.734383E-657.9271322
ACGTACT1600.050.8599741
ACGCGAT1400.049.66052224
TACGTAC1700.049.575640
CGTACTG1650.049.31875642
GGGAAGC91150.048.8639877
GGAAGCA86350.048.800258
AAGCAGT62250.048.69078410
GAAGCAT19750.048.617869
GAAGCAG64900.048.3960579
ATTAGCG302.5545758E-548.32127829
CATTGGT302.5918747E-548.20294613
CTACGTA1750.048.15704339
TGGGTAC3850.048.0777446
GTACCTG1750.047.9275059
AAGCATG16200.047.8458910
TGGGAAG107950.047.6090666
Produced by FastQC (version 0.11.5)