##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526558_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1103880 Sequences flagged as poor quality 0 Sequence length 64 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.90980450773635 39.0 38.0 40.0 34.0 40.0 2 37.572635612566586 39.0 38.0 40.0 33.0 40.0 3 37.32849132152045 39.0 38.0 40.0 33.0 40.0 4 37.161375330651886 39.0 38.0 40.0 32.0 40.0 5 37.31366634054426 39.0 38.0 40.0 33.0 40.0 6 37.52718683190202 39.0 38.0 40.0 34.0 40.0 7 37.20985433199261 39.0 38.0 40.0 33.0 40.0 8 37.1683090553321 39.0 38.0 40.0 33.0 40.0 9 37.26855002355328 39.0 38.0 40.0 33.0 40.0 10 36.22393919628945 39.0 35.0 40.0 30.0 40.0 11 36.13933851505598 38.0 35.0 40.0 30.0 40.0 12 35.93469942385042 38.0 35.0 40.0 29.0 40.0 13 35.743423198173716 38.0 35.0 40.0 29.0 40.0 14 36.18071982461862 38.0 35.0 40.0 30.0 40.0 15 35.810144218574486 38.0 35.0 40.0 29.0 40.0 16 35.66284288147262 38.0 35.0 40.0 28.0 40.0 17 35.30407290647534 38.0 35.0 40.0 27.0 40.0 18 35.529072000579774 38.0 35.0 40.0 28.0 40.0 19 35.635705873826865 38.0 35.0 40.0 28.0 40.0 20 35.60164872993441 38.0 35.0 40.0 28.0 40.0 21 35.95102728557452 39.0 35.0 40.0 29.0 40.0 22 35.84191669384354 38.0 35.0 40.0 29.0 40.0 23 35.730710765662934 38.0 35.0 40.0 29.0 40.0 24 35.66371616480052 38.0 35.0 40.0 29.0 40.0 25 35.63158404899083 38.0 35.0 40.0 29.0 40.0 26 35.71633601478422 39.0 35.0 40.0 29.0 40.0 27 35.17863354712469 39.0 35.0 40.0 26.0 40.0 28 35.089907417472915 39.0 35.0 40.0 26.0 40.0 29 34.87783364133783 38.0 35.0 40.0 26.0 40.0 30 34.76206924665725 38.0 34.0 40.0 25.0 40.0 31 34.15065949197376 38.0 33.0 40.0 22.0 40.0 32 34.10519440518897 38.0 33.0 39.0 23.0 40.0 33 34.03759647787803 38.0 33.0 39.0 23.0 40.0 34 33.50921929919919 38.0 33.0 39.0 19.0 40.0 35 33.631911983186576 38.0 33.0 39.0 21.0 40.0 36 33.32056835887959 38.0 33.0 39.0 15.0 40.0 37 33.20177736710512 38.0 33.0 39.0 15.0 40.0 38 33.256250679421676 38.0 33.0 39.0 16.0 40.0 39 32.66858625937602 38.0 32.0 39.0 9.0 40.0 40 32.790110338080225 37.0 32.0 39.0 11.0 40.0 41 32.7534097909193 38.0 32.0 39.0 4.0 40.0 42 32.488183498206325 37.0 32.0 39.0 4.0 40.0 43 32.46272058557089 37.0 32.0 39.0 4.0 40.0 44 32.33257328695148 37.0 32.0 39.0 4.0 40.0 45 32.20128003043809 37.0 32.0 39.0 4.0 40.0 46 32.0800304380911 37.0 32.0 39.0 4.0 40.0 47 31.91704533101424 37.0 32.0 39.0 4.0 40.0 48 31.690210892488313 37.0 32.0 39.0 4.0 40.0 49 31.409841649454652 36.0 31.0 39.0 4.0 40.0 50 30.92275881436388 36.0 31.0 39.0 4.0 40.0 51 30.29645613653658 36.0 30.0 39.0 4.0 40.0 52 30.116987353697866 35.0 29.0 39.0 4.0 40.0 53 29.688184404101897 35.0 28.0 39.0 4.0 40.0 54 29.19537449722796 35.0 27.0 39.0 4.0 40.0 55 28.62300250027177 35.0 24.0 39.0 4.0 40.0 56 27.224896727905207 35.0 4.0 39.0 4.0 40.0 57 26.645376309019095 34.0 4.0 39.0 4.0 40.0 58 26.24831231655615 34.0 4.0 39.0 4.0 40.0 59 25.619493060839947 33.0 4.0 39.0 4.0 40.0 60 24.9036761242164 33.0 4.0 38.0 4.0 40.0 61 23.953589158241837 32.0 4.0 38.0 4.0 40.0 62 23.672763343841723 32.0 4.0 37.0 4.0 40.0 63 23.369291046128204 32.0 4.0 37.0 4.0 39.0 64 23.35837409863391 32.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.34620894776275435 2 2 -0.6029679329444733 2 3 -0.8451111268864935 2 4 -0.4456044635355667 2 5 -0.8097967337821714 2 6 -0.45944186053564096 2 7 -0.5246683779124979 2 8 -0.3366396070252975 2 9 -0.6842812351585579 2 10 -0.5882415510200119 2 11 -0.6830755609959169 2 12 -0.3309336715106497 2 13 -0.21998529713494008 2 14 -0.4712810160048164 2 15 0.005141784741262256 2 16 -0.3003214719869831 2 17 0.22672643082871247 2 18 -0.3749705937761334 2 19 -0.49686352332593486 2 20 -0.1586692333428701 2 21 -0.3884628020052716 2 22 -0.8171088522642123 2 23 -0.499143027758592 2 24 -0.6685972273479024 2 25 -0.5151222756493823 2 26 -0.22182782488552277 2 27 0.01712991619411497 2 28 -0.05612934096225075 2 29 -0.03688549720190082 2 30 0.010649023932820967 2 31 0.08542666423108614 2 32 -0.3676370404160565 2 33 -0.42447456077093193 2 34 -0.2792231736696351 2 35 -0.13947415815000852 2 36 -1.2185225562836344 2 37 -1.2455530419824683 2 38 -1.2947223598732691 2 39 -5.137143388481608 2 40 -1.7349326576745163 2 41 -1.8224310586444439 2 42 -1.997101193669966 2 43 -2.392288711599651 2 44 -1.8091467178485843 2 45 -2.1263074990675648 2 46 -1.3313985173722678 2 47 -1.401152459364205 2 48 -1.5341281630745272 2 49 -1.52651696419424 2 50 -1.7249018039343902 2 51 -1.6177811017565915 2 52 -1.9711491848009501 2 53 -1.9571601039559852 2 54 -2.112095131393094 2 55 -2.001354530198874 2 56 -1.8684347765188285 2 57 -1.8768832111402673 2 58 -1.6652405524248302 2 59 -1.3136427746097468 2 60 -1.4277616452280633 2 61 -1.3106887367217546 2 62 -1.292316361865396 2 63 -1.3318836641392657 2 64 -1.4970677674546167 3 1 -0.35748079459585114 3 2 -0.2713097778107638 3 3 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120 27 -18.535410996811912 120 28 -16.461383432262856 120 29 -17.726807977822055 120 30 -30.523373370554687 120 31 -29.950749046363228 120 32 -29.896491476247235 120 33 -29.837049926834673 120 34 -29.364494491499094 120 35 -29.42887984290453 120 36 -29.090184916318087 120 37 -28.97251255963966 120 38 -28.99670341069153 120 39 -28.50234580019565 120 40 -28.446388299362717 120 41 -28.491681704639277 120 42 -28.265835043799164 120 43 -28.23294331969612 120 44 -28.09080046461861 120 45 -27.96523945427859 120 46 -27.853362341661672 120 47 -27.660411718623465 120 48 -27.464360721214064 120 49 -27.18026373422008 120 50 -26.71025925440812 120 51 -26.052750955331096 120 52 -25.877264602544276 120 53 -25.45414546140646 120 54 -24.969114941901278 120 55 -24.376031532782854 120 56 -22.990743131385322 120 57 -22.42124152294044 120 58 -22.029581637696147 120 59 -21.376519604928436 120 60 -20.678407640060104 120 61 -19.74565859029626 120 62 -19.46285899752431 120 63 -19.16995429290757 120 64 -19.14736923170957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 7089.0 5 484.0 6 1051.0 7 1356.0 8 4457.0 9 2362.0 10 3217.0 11 3183.0 12 3569.0 13 4860.0 14 5594.0 15 6281.0 16 7475.0 17 8032.0 18 8773.0 19 10009.0 20 10677.0 21 11327.0 22 13673.0 23 13078.0 24 13935.0 25 16990.0 26 18306.0 27 21798.0 28 26652.0 29 32063.0 30 39208.0 31 46269.0 32 53682.0 33 65189.0 34 75008.0 35 76370.0 36 90796.0 37 108651.0 38 134016.0 39 157213.0 40 1187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.17915910235644 0.46971195831385637 0.22638576351520293 0.12474317581449956 8 98.56014488287185 0.9120442045038414 0.307731074765508 0.22007983785880808 9 96.489292314382 2.2560423234409535 0.6413740623980867 0.6132912997789615 10 47.05656411928833 35.38727035547342 5.917491031633873 11.638674493604377 11 31.704261332753557 45.655053085480304 14.916657607710984 7.724027974055151 12 55.46073848606733 16.908178425191146 20.12582889444505 7.505254194296482 13 28.901239265137512 18.830941769032865 30.188154509548138 22.07966445628148 14 37.55063956227126 25.823277892524548 16.484944015653877 20.141138529550314 15 28.423245920249236 26.953556396581334 17.040927167449762 27.582270515719664 16 30.347449065117814 32.876138870892476 23.206371787262768 13.570040276726939 17 42.66872173002709 20.44845498304482 21.379493031956294 15.503330254971795 18 31.18898793347103 19.95244048266116 32.10539189042287 16.753179693444938 19 35.441171141790775 17.48432800666739 25.877541037069246 21.19695981447259 20 16.09063975138397 24.69534569769233 29.764159063522122 29.44985548740158 21 19.299289777874407 25.58629561184187 40.89031416458311 14.22410044570062 22 20.484654129072 32.43559082508968 28.041091422980756 19.03866362285756 23 26.573655264752748 22.771517530453973 35.551360248722915 15.103466956070367 24 32.442441735109675 24.06893040464271 23.6657009452474 19.82292691500021 25 21.095818396878414 14.626954957241328 34.08412150985466 30.193105136025594 26 29.554914855561616 13.966243043562566 42.47064750365604 14.00819459721978 27 32.40209114259523 15.362274117572314 34.381506457673424 17.85412828215903 28 48.76447435038952 18.629425780448987 22.662010194878608 9.944089674282882 29 53.344670739062806 18.02475182418858 19.48637017083203 9.144207265916583 30 50.905137127412594 24.095089769490897 15.396679318438006 9.603093784658503 31 46.280903201440935 25.840668758352216 17.28463991052234 10.593788129684505 32 35.256143878260204 32.693707050012975 21.90147656316631 10.148672508560509 33 30.5327344262057 39.05771985598632 18.975872442949193 11.43367327485879 34 21.194864474126877 45.7109084461579 20.50218678111276 12.59204029860246 35 18.486823406127495 52.78910094412355 19.576147827026617 9.147927822722348 36 17.33871737334464 52.683340467822546 19.35891388535194 10.619028273480877 37 14.96024769512991 54.05858942495968 21.759682636069602 9.221480243840801 38 16.237643019243126 53.91810697007786 20.529878968162002 9.314371042517012 39 13.974472834303201 53.325935476341144 21.115805943937982 11.583785745417675 40 14.468703294945035 53.97186151121649 22.321061684309285 9.238373509529195 41 12.9016386136948 55.62282690647887 21.56691013499441 9.908624344831914 42 14.196447428791995 53.52580675702249 22.807955050288037 9.469790763897487 43 15.319418147392952 53.57558986309351 21.479846344305273 9.625145645208272 44 14.405326908238584 53.42594884188152 21.863762756773284 10.30496149310661 45 14.64132828094806 53.80835828123023 22.151001754013887 9.39931168380782 46 14.441317665652765 53.692699452019625 21.95827191807698 9.907710964250631 47 16.294042566363142 54.07574110590973 20.711818547630884 8.918397780096248 48 16.40032623516968 54.52105145303907 19.911086352191983 9.16753595959927 49 17.552631674781587 54.88652461607374 19.027611372301955 8.533232336842717 50 15.799260063384073 55.71463858589891 19.211007719294408 9.275093631422605 51 15.557340677836432 56.837291568758175 18.864188625492275 8.741179127913119 52 16.058162116469976 56.22228618690723 18.666038760170082 9.053512936452705 53 15.493319630010276 56.5347721822542 18.216855087358685 9.755053100376841 54 15.108570387409753 57.027990980936984 17.802992689092857 10.060445942560412 55 15.454953883559291 56.94999958991407 17.524976511189422 10.070070015337214 56 15.346236857072027 56.79449273037493 17.54006679013606 10.319203622416987 57 15.505701536936042 56.68481510105282 17.17414841494357 10.635334947067571 58 15.870794073152739 56.17255299611449 16.79553033501514 11.161122595717634 59 16.25760325378225 55.451726747950424 17.224527389952307 11.066142608315019 60 17.05873874005375 54.06699132046761 17.106177849220266 11.76809209025838 61 17.914527452876534 52.45900593435299 17.32200358923085 12.30446302353963 62 18.12212941538989 51.366940711203 16.944995997819312 13.56593387558779 63 18.8630462687438 51.47370397922866 16.45552540988389 13.207724342143647 64 19.548772020106078 50.555982872059616 16.397639753026265 13.49760535480804 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 39.5 3 79.0 4 140.0 5 299.5 6 398.0 7 2116.0 8 3834.0 9 4754.5 10 5025.5 11 4376.0 12 3620.5 13 3232.0 14 3599.0 15 3774.5 16 4201.0 17 4452.0 18 4948.5 19 5445.0 20 6948.0 21 10339.0 22 12227.0 23 14851.5 24 24015.5 25 30555.0 26 41420.0 27 60946.5 28 69608.0 29 71911.5 30 70123.0 31 66031.0 32 64139.0 33 62247.0 34 59675.5 35 54798.5 36 52493.0 37 49412.0 38 43354.0 39 40377.0 40 37977.0 41 34313.0 42 33049.0 43 32878.0 44 32707.0 45 32497.0 46 30915.5 47 29544.0 48 29062.0 49 28955.5 50 29331.0 51 30578.5 52 36144.0 53 40462.0 54 42763.0 55 41759.5 56 38455.0 57 33271.5 58 28088.0 59 24206.5 60 18061.0 61 15797.0 62 13263.0 63 9439.5 64 8150.0 65 7848.5 66 5870.5 67 4194.0 68 3481.0 69 2768.0 70 2302.5 71 1644.5 72 1452.0 73 1098.0 74 605.5 75 467.0 76 367.5 77 209.5 78 151.0 79 121.5 80 75.5 81 59.0 82 40.5 83 22.0 84 18.0 85 11.5 86 9.0 87 7.5 88 3.0 89 0.0 90 1.5 91 1.5 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0010870746820306556 8 0.05833967460231184 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.09004601949487263 16 0.02400623256151031 17 0.141500887777657 18 0.0 19 0.0 20 0.015400224662100954 21 0.0 22 0.0 23 9.058955683588796E-5 24 0.0038047613871072944 25 0.05390078631735334 26 0.020473239844910677 27 0.18933217378700584 28 0.15925644091749103 29 0.12093705837591041 30 0.18643330796825744 31 0.2152407870420698 32 0.1563575750987426 33 0.20998659274558829 34 0.37051128745878176 35 0.23979055694459545 36 0.32530709859767365 37 0.3458709279994202 38 0.32530709859767365 39 0.6491647642859731 40 0.3525745552052759 41 0.3943363409066203 42 0.4899083233684821 43 0.4819364423669239 44 0.43990288799507193 45 0.47622930028626304 46 0.49670254013117365 47 0.49407544298293293 48 0.4788563974345038 49 0.4975178461426966 50 0.6122948146537667 51 0.5359278182411131 52 0.5482479979707939 53 0.5739754321121862 54 0.5621082001666847 55 0.5932710077182302 56 0.6080371054824799 57 0.600789940935609 58 0.6561401601623364 59 0.662209660470341 60 0.700981990796101 61 0.6077653368119723 62 0.6321339276008262 63 0.6326774649418415 64 0.7150233721056637 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 1103880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.02339217872297 #Duplication Level Percentage of deduplicated Percentage of total 1 89.71378933411486 55.643535397091405 2 5.536053548849091 6.867296407653566 3 1.9586170976667026 3.6444022912960206 4 0.870915495826531 2.1606853340870367 5 0.4790877072649893 1.4857322377850828 6 0.27908608089200654 1.0385919268072636 7 0.19003612667837552 0.8250679649168855 8 0.1344958850331651 0.6673512819069148 9 0.10429772342605532 0.582200874306199 >10 0.6082332760369041 7.1948992212453735 >50 0.07063217810134366 3.0358960156462516 >100 0.044002521273717426 5.326737885142906 >500 0.004386102235864138 1.9695763991634596 >1k 0.00580097392485257 7.439003357560438 >5k 5.659486755953727E-4 2.1190234053911983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7760 0.7029749610464905 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6015 0.544896184367866 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5351 0.48474471862883645 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5021 0.45485016487299346 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4448 0.4029423488060296 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4079 0.36951480233358697 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3718 0.33681197231583143 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3614 0.32739065840489906 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3542 0.3208682103127151 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3094 0.2802840888502373 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3061 0.27729463347465305 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2813 0.25482842337935285 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2780 0.25183896800376854 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2543 0.2303692430336631 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2538 0.22991629524948365 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2484 0.22502445918034567 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2457 0.22257854114577671 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2400 0.21741493640613108 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2311 0.20935246584773706 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2294 0.207812443381527 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2261 0.20482298800594267 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2166 0.19621698010653332 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2089 0.18924158423016996 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1997 0.18090734500126826 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1742 0.1578070080081168 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1681 0.15228104504112766 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1658 0.15019748523390225 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1634 0.14802333586984093 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1582 0.14331267891437477 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1525 0.13814907417472913 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1498 0.13570315614016018 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1439 0.13035837228684277 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1407 0.12745950646809437 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1282 0.11613581186360837 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1269 0.11495814762474182 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1245 0.1127839982606805 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1216 0.11015690111243975 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1193 0.10807334130521434 No Hit ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1185 0.10734862485052724 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1166 0.10562742327064535 No Hit ATGCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG 1104 0.10001087074682032 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0016306120230459833 0.0 0.0 0.0 0.0 8 0.0034424031597637423 0.0 0.0 0.0 0.0 9 0.0067036272058557085 0.0 0.0 0.0 0.0 10 0.03868174076892416 0.0 0.0 0.0 0.0 11 0.06893865275211074 0.0 0.0 0.0 0.0 12 0.08053411602710439 0.0 0.0 0.0 0.0 13 0.09068014639272384 0.0 0.0 0.0 0.0 14 0.1098851324419321 0.0 0.0 0.0 0.0 15 0.12809363336594556 0.0 0.0 0.0 0.0 16 0.14929158966554334 0.0 0.0 0.0 0.0 17 0.16052469471319347 0.0 0.0 0.0 0.0 18 0.17565315070478676 0.0 0.0 0.0 0.0 19 0.19005689024169295 0.0 0.0 0.0 0.0 20 0.21804906330398233 0.0 0.0 0.0 0.0 21 0.23788817625104178 0.0 0.0 0.0 0.0 22 0.2602637967895061 0.0 0.0 0.0 0.0 23 0.28771243251078016 0.0 0.0 0.0 0.0 24 0.31651991158459253 0.0 0.0 0.0 0.0 25 0.33889553212305684 0.0 0.0 0.0 0.0 26 0.36226763778671595 0.0 0.0 0.0 0.0 27 0.3813820342790883 0.0 0.0 0.0 0.0 28 0.4415335000181179 0.0 0.0 0.0 0.0 29 0.4581113889190854 0.0 0.0 0.0 0.0 30 0.4881871217886002 0.0 0.0 0.0 0.0 31 0.5101097945428851 0.0 0.0 0.0 0.0 32 0.5390984527303693 0.0 0.0 0.0 0.0 33 0.5723448200891401 0.0 0.0 0.0 0.0 34 0.6075841576983005 0.0 0.0 0.0 0.0 35 0.6960901547269631 0.0 0.0 0.0 0.0 36 0.7268000144943291 0.0 0.0 0.0 0.0 37 0.7605899191941153 0.0 0.0 0.0 0.0 38 0.7927492118708556 0.0 0.0 0.0 0.0 39 0.8275356016958365 0.0 0.0 0.0 0.0 40 0.8665797006921042 0.0 0.0 0.0 0.0 41 0.9170380838496938 0.0 0.0 0.0 0.0 42 0.9747436315541544 0.0 0.0 0.0 0.0 43 1.0351668659636917 0.0 0.0 0.0 0.0 44 1.1012972424538898 0.0 0.0 0.0 0.0 45 1.1775736493097075 0.0 0.0 0.0 0.0 46 1.2598289669166938 0.0 0.0 0.0 0.0 47 1.353498568685002 0.0 0.0 0.0 0.0 48 1.4526941334202994 0.0 0.0 0.0 0.0 49 1.546001376961264 0.0 0.0 0.0 0.0 50 1.6248142914084864 0.0 0.0 0.0 0.0 51 1.7010001087074682 0.0 0.0 0.0 0.0 52 1.764141029822082 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGT 15 0.004720558 57.89612 14 GGACTAC 35 1.1182292E-6 49.530746 9 GGAAGTA 105 0.0 49.51279 8 CACTCAA 30 2.5094638E-5 48.46692 57 GGGAAGC 9090 0.0 48.042114 7 AAGCAGT 6145 0.0 47.835278 10 TGGGTGC 2625 0.0 47.75234 6 GGAAGCA 8600 0.0 47.588894 8 GAAGCAG 6500 0.0 47.51777 9 TGGGTAT 415 0.0 47.325474 6 TGGGAAG 10745 0.0 47.20112 6 TTGGGTG 4735 0.0 46.41933 5 CATCTAA 25 6.041516E-4 46.40965 27 TGGGACT 625 0.0 46.211937 6 TGGGGTG 13035 0.0 45.888435 6 GAAGCAT 1935 0.0 45.840473 9 AAGCATG 1515 0.0 45.787594 10 TGGGCAT 585 0.0 45.42199 6 TTGGGAT 2175 0.0 45.41518 5 TGGGATG 1075 0.0 45.405914 6 >>END_MODULE