FastQCFastQC Report
Fri 17 Jun 2016
SRR1526557_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526557_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928815
Sequences flagged as poor quality0
Sequence length56
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA111171.196901428163843No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA84530.9100843547961651No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA79130.8519457588432573No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA73040.7863783422963668No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA58680.6317727426882641No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT55480.5973202413828373No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA54400.5856925221922558No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA52330.5634060604103077No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA48870.526154293373815No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA42630.4589719158282327No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAA38850.4182748986611974No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAA35960.38715998341973373No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAA34070.36681147483621607No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA33620.36196659184014035No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAA32810.35324580244720427No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA32800.3531381383806248No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA32380.3486162475842875No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31820.3425870598558378No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAA31640.34064910665740755No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAA31380.3378498409263416No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAA30880.3324666375973687No Hit
ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29280.3152403869446553No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA27530.29639917529325No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAA27340.2943535580282403No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAA24140.2599010567228135No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA23510.25311822052830757No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAA22110.23804525120718337No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAA21010.2262022038834429No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA20880.2248025710179099No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA20850.22447957881817157No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAA19250.20725332816545813No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA19020.2047770546341306No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAA18620.20047049197095224No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAA17920.19293400731039012No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGCAAAAAAAAAAAAAAAAAAAAAAAAA17800.19164203851143663No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAA16790.1807679677869113No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAA16500.17764570985610698No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAA16150.17387746752582592No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAA15400.16580266253236653No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAA14880.16020413107023465No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA14530.1564358887399536No Hit
ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14470.15578990434047685No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAA13340.143623864816998No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA13110.14114759128567045No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAA13090.14093226315251153No Hit
ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG12160.13091950496062185No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGCAAAAAAAAAAAAAAAAAAAA12010.12930454396192997No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAA11850.12758191889665865No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAAA11610.12499798129875163No Hit
ATGCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA11590.12478265316559273No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA11280.1214450671016295No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA10260.1104633323105247No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAAA9890.10647976184708471No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC9750.10497246491497231No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAA9670.10411115238233663No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAA9630.1036804961160188No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGTAAAAAAAAAAAAAAAAAAAA9570.10303451171654204No Hit
ATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9400.10120422258469125No Hit
ATGCTTGGGGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA9290.10001991785231719No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAT150.00801938550.6363836
CTTCAAT150.00803155350.6169635
ACCCATG150.00814344250.44011331
TATACTA402.59206E-950.19350444
ACTCCGT204.1077912E-450.1853243
TAGGACT150.00858434149.7717120
CCTTATG150.00860629349.7395618
GACGTGA150.00860629349.739569
GATTAAT252.1526375E-549.73955514
TAGCATA2000.046.1781330
TGGGTAC3700.045.738636
GATTAAG2000.045.17660550
GACTAAT457.961717E-944.212949
CCTGGTT2250.044.21293612
GGGTACC2550.043.9185757
ACGCGAA401.5507248E-743.60897424
ATCCTGC2300.043.27974720
GTTGATC2300.043.2517916
ACCTGGT2300.043.2517911
GTAGCAT2150.042.9471229
Produced by FastQC (version 0.11.5)