##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526557_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 928815 Sequences flagged as poor quality 0 Sequence length 56 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.07483621603872 39.0 38.0 40.0 35.0 40.0 2 37.797484967404706 39.0 38.0 40.0 34.0 40.0 3 37.54369384646027 39.0 38.0 40.0 33.0 40.0 4 37.49698917437811 39.0 38.0 40.0 33.0 40.0 5 37.339605841852254 39.0 38.0 40.0 33.0 40.0 6 37.772784677250044 39.0 38.0 40.0 35.0 40.0 7 37.29594052636962 39.0 38.0 40.0 33.0 40.0 8 37.60044895915764 39.0 38.0 40.0 34.0 40.0 9 37.51559244844237 39.0 38.0 40.0 33.0 40.0 10 36.399752372646866 39.0 35.0 40.0 30.0 40.0 11 36.27355932020908 38.0 35.0 40.0 30.0 40.0 12 36.14224576476478 38.0 35.0 40.0 30.0 40.0 13 36.00438408079111 38.0 35.0 40.0 29.0 40.0 14 36.502383144113736 39.0 36.0 40.0 31.0 40.0 15 36.35465620171939 38.0 35.0 40.0 31.0 40.0 16 36.30048502661994 39.0 36.0 40.0 30.0 40.0 17 36.13450687165905 39.0 35.0 40.0 30.0 40.0 18 36.01348169441708 39.0 35.0 40.0 29.0 40.0 19 36.09707961219403 39.0 35.0 40.0 29.0 40.0 20 36.11398287064701 39.0 35.0 40.0 30.0 40.0 21 36.27116594800902 39.0 36.0 40.0 30.0 40.0 22 36.19243229276013 39.0 36.0 40.0 30.0 40.0 23 36.01513649112041 39.0 35.0 40.0 29.0 40.0 24 35.975060695617536 39.0 35.0 40.0 29.0 40.0 25 35.89475299171525 39.0 35.0 40.0 29.0 40.0 26 35.72143214741364 39.0 35.0 40.0 29.0 40.0 27 35.60282725838837 39.0 35.0 40.0 29.0 40.0 28 35.392704682848574 38.0 35.0 40.0 28.0 40.0 29 35.32713726630168 38.0 35.0 40.0 28.0 40.0 30 35.037731948773434 38.0 35.0 40.0 27.0 40.0 31 34.276801085253794 38.0 34.0 40.0 24.0 40.0 32 33.84047845911188 38.0 33.0 39.0 22.0 40.0 33 33.769655959475244 38.0 33.0 39.0 22.0 40.0 34 33.80463709134758 38.0 33.0 39.0 22.0 40.0 35 33.63947180008936 38.0 33.0 39.0 20.0 40.0 36 33.19878770261032 38.0 33.0 39.0 12.0 40.0 37 32.76120217696742 38.0 33.0 39.0 4.0 40.0 38 32.903929200109815 38.0 33.0 39.0 6.0 40.0 39 32.67537561301228 38.0 32.0 39.0 4.0 40.0 40 32.275650156382056 37.0 32.0 39.0 4.0 40.0 41 32.15117649908755 37.0 32.0 39.0 4.0 40.0 42 32.075024628155234 37.0 32.0 39.0 4.0 40.0 43 31.929182883566696 37.0 32.0 39.0 4.0 40.0 44 31.485274247293596 37.0 31.0 39.0 4.0 40.0 45 31.530485618772307 37.0 31.0 39.0 4.0 40.0 46 31.231813655033566 37.0 31.0 39.0 4.0 40.0 47 31.04483562388635 36.0 31.0 39.0 4.0 40.0 48 30.555276346742893 36.0 30.0 39.0 4.0 40.0 49 30.481466169258677 36.0 30.0 39.0 4.0 40.0 50 30.080435824141514 36.0 29.0 39.0 4.0 40.0 51 29.258064307746967 35.0 28.0 39.0 4.0 40.0 52 29.00393942819614 35.0 27.0 39.0 4.0 40.0 53 28.50953526805661 35.0 23.0 39.0 4.0 40.0 54 27.952634270549034 35.0 15.0 39.0 4.0 40.0 55 27.269864289444076 35.0 4.0 39.0 4.0 40.0 56 15.45346812874469 18.0 4.0 22.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.2817127339887051 1 2 0.22166194504699632 1 3 -0.13810876016723483 1 4 0.009491703463098133 1 5 0.40863244088272666 1 6 -0.7313182275281136 1 7 -0.41545262590318544 1 8 -1.0776603095032868 1 9 -1.274906184680873 1 10 0.2901717084156701 1 11 -0.35505846901018145 1 12 -0.3533079560175594 1 13 -0.6316415566138218 1 14 -0.6310215099883578 1 15 -0.6580484409261302 1 16 -0.6349472979987851 1 17 -0.9265509438570518 1 18 -1.1729333205520263 1 19 -1.2207490262054819 1 20 -0.9988653444157833 1 21 -1.1171051678900525 1 22 -0.9787693803900268 1 23 -1.237229474526309 1 24 -0.8750582714826081 1 25 -1.056749368678389 1 26 -1.018274302969715 1 27 -0.9727667230757504 1 28 -0.9008192556895125 1 29 -1.0481250741566939 1 30 -1.010651819182229 1 31 -0.9545232704374698 1 32 -1.1460628079402824 1 33 -1.1725081907506691 1 34 -1.2896310300663245 1 35 -1.28859980631006 1 36 -1.3095929399505728 1 37 -1.0103022032055087 1 38 -1.3318871296753336 1 39 -1.4969201608524543 1 40 -1.3193797143997088 1 41 -1.1921251491012015 1 42 -1.2855556308201095 1 43 -1.221556836601966 1 44 -1.0530555158860437 1 45 -1.2617514218163102 1 46 -1.3700003780162717 1 47 -1.3154982563508248 1 48 -1.3082299765161238 1 49 -1.2377308305135237 1 50 -1.2548564528657558 1 51 -0.9841827411586408 1 52 -1.0801084261748848 1 53 -0.9511777235791925 1 54 -1.1290427750916479 1 55 -1.0389994381282825 1 56 0.5845750110888002 2 1 0.0872219856393528 2 2 0.7769079680272597 2 3 0.3657569434949153 2 4 0.447387453791265 2 5 0.693996256508612 2 6 0.3730687823197485 2 7 0.6667694742408017 2 8 0.4497523579768057 2 9 0.42637640779820885 2 10 0.669489181858836 2 11 -0.3769371373807999 2 12 -0.45103479504584243 2 13 -0.32369503459058535 2 14 -0.3412281820136158 2 15 -0.6079966861800159 2 16 -0.7038345998706319 2 17 -0.7688391975536106 2 18 -0.8131554322613184 2 19 -0.9277347604000425 2 20 -0.9302079030085153 2 21 -1.1079049666537202 2 22 -1.067961023299624 2 23 -1.094173862750516 2 24 -1.2377303776983837 2 25 -1.1322809390735173 2 26 -1.0924382758837652 2 27 -1.1835328667978118 2 28 -1.1355241379836087 2 29 -1.3846624792488953 2 30 -1.3362383247827267 2 31 -1.5004317541679768 2 32 -1.6337674415802894 2 33 -1.181209638148765 2 34 -1.7149581836709373 2 35 -1.4190882670256073 2 36 -1.5835707114316868 2 37 -1.3783695248540262 2 38 -1.291236523364148 2 39 -1.4630367044449848 2 40 -1.420097630794107 2 41 -1.279963651162717 2 42 -1.4585234619203753 2 43 -1.4085895259404886 2 44 -1.1802579839224272 2 45 -1.352536185681668 2 46 -1.3512660273214827 2 47 -1.2763612595139335 2 48 -0.8689969876361658 2 49 -1.3954396854903663 2 50 -1.3287746630189403 2 51 -1.4320159810057476 2 52 -1.0661331990611806 2 53 -1.007979280269435 2 54 -1.3224580678962923 2 55 -1.2227533631041396 2 56 -0.807870530774716 3 1 0.0240141764254318 3 2 0.6366304658117414 3 3 0.17182019495319167 3 4 0.2246912644866086 3 5 0.5415816697858631 3 6 0.17308597740459675 3 7 0.35090502441737925 3 8 0.24874181606709556 3 9 0.3174521587604744 3 10 0.2708423027667308 3 11 -0.5698739256034884 3 12 -0.7609669405956225 3 13 -0.57155953732196 3 14 -0.657466955607255 3 15 -0.4651240488508108 3 16 -1.1665622694075566 3 17 -1.0451179166520603 3 18 -1.0294775358815471 3 19 -0.8784946508805831 3 20 -1.094770155829167 3 21 -0.8103876723664527 3 22 -0.8520264704379699 3 23 -0.737775307137639 3 24 -0.846637431651267 3 25 -0.9975481903730667 3 26 -0.8081056435808165 3 27 -0.6254439020741955 3 28 -0.5542270995062992 3 29 -0.49494249459220185 3 30 -0.8933815598395398 3 31 -0.7174297568927344 3 32 -0.5110342167082607 3 33 -0.6313108246096135 3 34 -0.5932381461062946 3 35 -0.6020810054090049 3 36 -0.4026241881455732 3 37 -0.12365344215889706 3 38 -0.2734541602304077 3 39 -0.18624610089750604 3 40 -0.24867850721435758 3 41 -0.7395750422450824 3 42 -0.7454181618661444 3 43 -0.8298598459013355 3 44 -0.6645006727046194 3 45 -0.7354879204011908 3 46 -0.8740984546061163 3 47 -0.8312320979727268 3 48 -0.44099148520901466 3 49 -0.826545593986058 3 50 -0.7824802038043579 3 51 -1.2524926816657747 3 52 -1.0534088362713447 3 53 -1.0200594887267727 3 54 -1.270323893326502 3 55 -1.0434787311450897 3 56 -1.0419657386368382 4 1 0.06965264938918381 4 2 0.6004402954719978 4 3 0.05405695782903308 4 4 0.2965805235823211 4 5 0.3540741429904699 4 6 0.19128811033115056 4 7 0.37110092181210774 4 8 0.2970069733603893 4 9 0.2676348089953109 4 10 0.19827596661001934 4 11 -0.3949231006667162 4 12 -0.6333285479176851 4 13 -0.2661616541913503 4 14 -0.4130822794936293 4 15 -0.5509442353815217 4 16 -0.9462901004861948 4 17 -1.296633311457569 4 18 -0.899299435176367 4 19 -0.9246951245669024 4 20 -1.1005320013324962 4 21 -1.0396866433698904 4 22 -1.1175892071138662 4 23 -1.5035655202964833 4 24 -0.975225873169741 4 25 -1.2360934113597821 4 26 -0.9873089406517224 4 27 -0.9872278529392702 4 28 -0.915831745378938 4 29 -1.0151209967264663 4 30 -0.9984663282818573 4 31 -1.0116277684806505 4 32 -0.9932308366753446 4 33 -1.0393233491971472 4 34 -0.8930307410777232 4 35 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-1.2050109457001597 120 24 -1.1766564399051873 120 25 -1.025777361026364 120 26 -1.3545687050578437 120 27 -1.0246019257260741 120 28 -1.2594869002385636 120 29 -1.196200356227422 120 30 -1.037534289246203 120 31 -1.497351172433575 120 32 -1.3952624688419064 120 33 -1.3752351998248926 120 34 -1.3834533603637595 120 35 -1.5883644452650998 120 36 -1.0890632660538984 120 37 -0.7098947831975906 120 38 -1.0281277668233386 120 39 -1.2656444705251246 120 40 -1.1833513483742415 120 41 -1.0963688983691853 120 42 -1.2145150784466985 120 43 -1.290016504530957 120 44 -1.0385448381912425 120 45 -1.2863611519319278 120 46 -1.2099283753837966 120 47 -1.305888609894346 120 48 -1.1090933388327002 120 49 -1.1057487781875217 120 50 -1.3555918166993521 120 51 -1.3410851778705037 120 52 -1.6196889592509187 120 53 -1.324641881215321 120 54 -1.2298771355733038 120 55 -1.342613791805661 120 56 -0.9335204607208567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 4471.0 5 804.0 6 586.0 7 750.0 8 1969.0 9 2627.0 10 1593.0 11 2705.0 12 2474.0 13 2510.0 14 3638.0 15 4214.0 16 5026.0 17 5836.0 18 6570.0 19 7138.0 20 7658.0 21 8726.0 22 9571.0 23 10081.0 24 12367.0 25 13589.0 26 14123.0 27 14863.0 28 18584.0 29 19786.0 30 23023.0 31 29399.0 32 35481.0 33 42426.0 34 53874.0 35 64389.0 36 80953.0 37 121240.0 38 144767.0 39 151004.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 100.0 0.0 7 99.11094784830635 0.4896088730701116 0.2805151827226778 0.11892809590085879 8 98.76939971899678 0.9272029413822989 0.14459284141621312 0.1588044982047017 9 96.5276185246793 2.226923553129525 0.646092063543332 0.599365858647847 10 47.345488606450154 35.294757298277915 5.929490802797113 11.43026329247482 11 31.85768963679527 45.43778901072872 14.941619159897288 7.762902192578716 12 54.94000419889859 17.309367312112744 20.105726113381028 7.644902375607629 13 28.697964610821312 19.177661859466095 29.887652546524336 22.236720983188256 14 36.967641564789545 26.381033898031365 16.75145211909799 19.899872418081102 15 28.416100084516295 27.359915591371802 16.989066714038856 27.23491761007305 16 30.075203350505753 33.07644687047475 23.373868854400502 13.474480924619003 17 42.552930346732126 20.89135080721134 21.367118317425966 15.188600528630566 18 31.047625199851424 20.48922551853706 31.63514801117553 16.828001270435987 19 34.755790980981146 18.171433493214472 25.952961569311434 21.11981395649295 20 16.060141147591285 25.56364830456011 29.51793414189047 28.85827640595813 21 19.15106883502097 26.005501633802208 40.572019185736664 14.271410345440158 22 20.52744626217277 32.48784741848485 27.864429407363144 19.120276911979243 23 26.397857913425078 23.575979690228614 34.819565596556465 15.206596799789839 24 31.941731893498133 24.70171509781333 23.547550090976628 19.80900291771191 25 20.926179643608037 15.472374452831488 33.75049546754903 29.85095043601144 26 29.542876533036015 14.819590544056101 41.60259828396917 14.034934638938712 27 32.37218866802033 15.546983786207283 34.41163030310869 17.6691972426637 28 48.28291210863825 19.431912485854394 22.429271972840436 9.855903432666919 29 53.28872822617075 18.421815821731943 19.34845350154763 8.941002450549677 30 50.32552027137839 24.601218785875204 15.764805399946482 9.308455542799928 31 46.123095050043375 26.300286147113283 17.201412177005512 10.375206625837832 32 34.94148653040336 33.05149984290599 21.632114982017864 10.374898644672784 33 30.438022580443096 39.07848723559131 18.906855132223246 11.576635051742349 34 20.921106355951427 46.01081468775451 20.516473711840202 12.551605244453853 35 18.328941440346426 52.648911102251695 19.81202914099327 9.21011831640861 36 17.57972632527757 52.334103207858554 19.494433450525744 10.591737016338127 37 14.986889991638414 53.64329424709692 21.949166788561 9.420648972703674 38 16.62850474112803 53.13243251720532 20.683475219264327 9.55558752240233 39 13.915476748047329 53.120433357165965 21.239520044824083 11.724569849962627 40 14.732445785666126 53.04994244164494 22.61002751094259 9.60758426174635 41 13.101900907755699 54.957913341638466 21.7001992581665 10.239986492439337 42 14.278429483188887 52.990055703219305 23.077481066860102 9.654033746731699 43 15.861187392686718 52.589872334432876 21.71094609014045 9.837994182739962 44 14.78722055392338 51.971091168588245 22.07218971718632 11.16949856030205 45 14.902118454876087 52.85007390966982 22.48024379353843 9.767563841915669 46 14.689502573159135 52.73995101945237 22.260672349624404 10.30987405776409 47 16.80883672734998 53.066320978447955 20.731863291292907 9.392979002909158 48 17.074110535724557 53.10204024039604 20.06039207088145 9.76345715299795 49 18.1674065023801 53.7431057474981 19.10881173089005 8.98067601923175 50 16.056203547984595 55.09445017088916 19.415830304345466 9.433515976780773 51 15.896554155570138 56.009047172216285 19.0482027694761 9.046195902737475 52 16.81546068625408 55.068981385010765 18.702898173230533 9.41265975550462 53 15.906004643756166 55.30433536646922 18.420124245986646 10.369535743787972 54 15.66648353199464 55.93866653347781 17.924794624961184 10.470055309566362 55 16.30445522784303 55.685733303075146 17.454772884469843 10.555038584611976 56 16.05960840181379 55.51455897426988 17.70455098247016 10.721281641446168 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 53.0 4 180.0 5 254.0 6 319.5 7 385.0 8 2142.0 9 3899.0 10 4664.0 11 5429.0 12 4866.0 13 3505.0 14 2707.0 15 3096.5 16 3486.0 17 3620.5 18 3755.0 19 4100.5 20 4446.0 21 5186.0 22 7878.5 23 9831.0 24 12684.0 25 15537.0 26 19936.5 27 24336.0 28 33397.5 29 56721.0 30 70983.0 31 80041.0 32 89099.0 33 87332.5 34 85566.0 35 73526.5 36 61487.0 37 53980.0 38 40720.0 39 34967.0 40 31254.5 41 27542.0 42 25744.0 43 23946.0 44 23697.0 45 23448.0 46 22426.5 47 22224.0 48 23043.0 49 25803.5 50 28564.0 51 33048.0 52 37532.0 53 42606.5 54 48819.5 55 49958.0 56 45683.0 57 41408.0 58 35713.5 59 30019.0 60 24702.5 61 19386.0 62 15746.5 63 10167.0 64 8227.0 65 6999.5 66 5772.0 67 5870.0 68 5968.0 69 4439.5 70 2911.0 71 2363.5 72 1518.0 73 1220.0 74 928.0 75 636.0 76 499.5 77 363.0 78 282.5 79 159.0 80 116.0 81 86.0 82 56.0 83 41.5 84 27.0 85 20.5 86 14.0 87 11.0 88 5.0 89 2.0 90 1.5 91 1.0 92 2.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05630830682105694 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0766406657945876E-4 24 5.383203328972938E-4 25 0.04295796256520405 26 0.05490867395552397 27 0.1036804961160188 28 0.1038958242491777 29 0.1365180364227537 30 0.21435915655970245 31 0.21737375042392726 32 0.28186452630502307 33 0.31018017581542073 34 0.3269757702018163 35 0.3494775601169232 36 0.3828534207565554 37 1.369594590957295 38 0.4571416266963819 39 0.4642474550906262 40 0.675376689652945 41 0.5262619574403945 42 0.5566232242158018 43 0.613685179502915 44 0.6515829309388845 45 0.6531978919375764 46 0.6899113386411718 47 0.6687015175250184 48 0.8076958274790997 49 0.7766885763042156 50 0.7714130370418221 51 0.7514951847246223 52 0.7974677411540512 53 0.8158782965391387 54 0.8416100084516293 55 0.8453782507819103 56 0.84742386804692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 928815.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.57293029470607 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6750506363645 33.49427446674416 2 11.152634886373509 9.49600695239773 3 4.201186297406348 5.365704341836643 4 1.8842730138166834 3.2087609469365357 5 1.0253534931421138 2.1826151395486293 6 0.6139520995114495 1.5682643962073626 7 0.411544907613921 1.2264470865492094 8 0.30392520536962697 1.0351189266404284 9 0.2185926902751208 0.837551822941349 >10 1.2697452494639834 9.99588004231266 >50 0.1302670632834491 3.8878334499454175 >100 0.08904946206942106 7.445605290311502 >500 0.011194789517049957 3.3070276715663245 >1k 0.011194789517049957 10.788269269278253 >5k 0.0017809892413488567 4.95647961819801 >10k+ 2.544270344784081E-4 1.2041605785857925 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 11117 1.196901428163843 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 8453 0.9100843547961651 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 7913 0.8519457588432573 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 7304 0.7863783422963668 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 5868 0.6317727426882641 No Hit ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 5548 0.5973202413828373 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 5440 0.5856925221922558 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 5233 0.5634060604103077 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 4887 0.526154293373815 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAA 4263 0.4589719158282327 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAA 3885 0.4182748986611974 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAA 3596 0.38715998341973373 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAA 3407 0.36681147483621607 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 3362 0.36196659184014035 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAA 3281 0.35324580244720427 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 3280 0.3531381383806248 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAA 3238 0.3486162475842875 No Hit ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3182 0.3425870598558378 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAA 3164 0.34064910665740755 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAA 3138 0.3378498409263416 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAA 3088 0.3324666375973687 No Hit ATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2928 0.3152403869446553 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2753 0.29639917529325 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAA 2734 0.2943535580282403 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAAAAA 2414 0.2599010567228135 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA 2351 0.25311822052830757 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAA 2211 0.23804525120718337 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAA 2101 0.2262022038834429 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 2088 0.2248025710179099 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 2085 0.22447957881817157 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAA 1925 0.20725332816545813 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAAAAAAA 1902 0.2047770546341306 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAA 1862 0.20047049197095224 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGTAAAAAAAAAAAAAAAAAAAAAAA 1792 0.19293400731039012 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGCAAAAAAAAAAAAAAAAAAAAAAAAA 1780 0.19164203851143663 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAA 1679 0.1807679677869113 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGCAAAAAAAAAAAAAAAAAAAAAAAA 1650 0.17764570985610698 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAA 1615 0.17387746752582592 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAA 1540 0.16580266253236653 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGCAAAAAAAAAAAAAAAAAAAAAA 1488 0.16020413107023465 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA 1453 0.1564358887399536 No Hit ATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1447 0.15578990434047685 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAA 1334 0.143623864816998 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 1311 0.14114759128567045 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAA 1309 0.14093226315251153 No Hit ATGCTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1216 0.13091950496062185 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGCAAAAAAAAAAAAAAAAAAAA 1201 0.12930454396192997 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAA 1185 0.12758191889665865 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGTAAAAAAAAAAAAAAAAAAAAAA 1161 0.12499798129875163 No Hit ATGCTTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA 1159 0.12478265316559273 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAA 1128 0.1214450671016295 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA 1026 0.1104633323105247 No Hit ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGGGAAAAAAAAAAAAAAAAAAAAAA 989 0.10647976184708471 No Hit ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 975 0.10497246491497231 No Hit ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGCAAAAAAAAAAAAAAAAAAAAAAA 967 0.10411115238233663 No Hit ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAAA 963 0.1036804961160188 No Hit ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGGTAAAAAAAAAAAAAAAAAAAA 957 0.10303451171654204 No Hit ATGCTTGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 940 0.10120422258469125 No Hit ATGCTTGGGGTGCAGTGCTATGCTTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAA 929 0.10001991785231719 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.003445250130542681 0.0 0.0 0.0 0.0 8 0.005167875195814021 0.0 0.0 0.0 0.0 9 0.008397797193197784 0.0 0.0 0.0 0.0 10 0.04209665003256838 0.0 0.0 0.0 0.0 11 0.08150169840065029 0.0 0.0 0.0 0.0 12 0.09571335518913884 0.0 0.0 0.0 0.0 13 0.10701808217998202 0.0 0.0 0.0 0.0 14 0.1287662236290327 0.0 0.0 0.0 0.0 15 0.150191372878345 0.0 0.0 0.0 0.0 16 0.17387746752582592 0.0 0.0 0.0 0.0 17 0.18916576498010906 0.0 0.0 0.0 0.0 18 0.2047770546341306 0.0 0.0 0.0 0.0 19 0.21985002395525483 0.0 0.0 0.0 0.0 20 0.24805800939907302 0.0 0.0 0.0 0.0 21 0.2710981196470772 0.0 0.0 0.0 0.0 22 0.3005980738898489 0.0 0.0 0.0 0.0 23 0.3322513094642098 0.0 0.0 0.0 0.0 24 0.3649811857043652 0.0 0.0 0.0 0.0 25 0.38715998341973373 0.0 0.0 0.0 0.0 26 0.4086927967356255 0.0 0.0 0.0 0.0 27 0.43259421951626537 0.0 0.0 0.0 0.0 28 0.48211969014281636 0.0 0.0 0.0 0.0 29 0.5010685658608012 0.0 0.0 0.0 0.0 30 0.5331524577014798 0.0 0.0 0.0 0.0 31 0.5584535133476527 0.0 0.0 0.0 0.0 32 0.5871998191243681 0.0 0.0 0.0 0.0 33 0.6229442892287484 0.0 0.0 0.0 0.0 34 0.6557818295354834 0.0 0.0 0.0 0.0 35 0.735022582537965 0.0 0.0 0.0 1.0766406657945877E-4 36 0.7697980760431302 0.0 0.0 0.0 1.0766406657945877E-4 37 0.8025279522832857 0.0 0.0 0.0 1.0766406657945877E-4 38 0.8352578285234411 0.0 0.0 0.0 1.0766406657945877E-4 39 0.8670187281643815 0.0 0.0 0.0 1.0766406657945877E-4 40 0.9024402060690234 0.0 0.0 0.0 1.0766406657945877E-4 41 0.946690137433181 0.0 0.0 0.0 1.0766406657945877E-4 42 0.9958926158599937 0.0 0.0 0.0 1.0766406657945877E-4 43 1.0548925243455372 0.0 0.0 0.0 1.0766406657945877E-4 44 1.1176606751613616 0.0 0.0 0.0 1.0766406657945877E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGAT 15 0.008019385 50.63638 36 CTTCAAT 15 0.008031553 50.61696 35 ACCCATG 15 0.008143442 50.440113 31 TATACTA 40 2.59206E-9 50.193504 44 ACTCCGT 20 4.1077912E-4 50.18532 43 TAGGACT 15 0.008584341 49.77171 20 CCTTATG 15 0.008606293 49.73956 18 GACGTGA 15 0.008606293 49.73956 9 GATTAAT 25 2.1526375E-5 49.739555 14 TAGCATA 200 0.0 46.17813 30 TGGGTAC 370 0.0 45.73863 6 GATTAAG 200 0.0 45.176605 50 GACTAAT 45 7.961717E-9 44.21294 9 CCTGGTT 225 0.0 44.212936 12 GGGTACC 255 0.0 43.918575 7 ACGCGAA 40 1.5507248E-7 43.608974 24 ATCCTGC 230 0.0 43.279747 20 GTTGATC 230 0.0 43.25179 16 ACCTGGT 230 0.0 43.25179 11 GTAGCAT 215 0.0 42.94712 29 >>END_MODULE