FastQCFastQC Report
Fri 17 Jun 2016
SRR1526556_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526556_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414418
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC13710.33082539851068243No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC11330.2733954606218842No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC10650.25698690693937043No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC6630.15998339840450945No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5670.13681838144096056No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT5670.13681838144096056No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC5230.126201081999334No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC5210.12571847747926007No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGC4850.11703159611792924No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG4780.11534248029767048No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4780.11534248029767048No Hit
GTCGGTGGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCCTGGAGGAG4630.11172294639711597No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4570.11027513283689415No Hit
GTCGGTGGGGCTCACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCCGTCGCCGC4450.10737950571645054No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG4390.10593169215622875No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTTATTAT4160.10038174017537849No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA4160.10038174017537849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAT150.004619996558.20536852
TTCGCAT150.004626665358.1841749
CGAAGTA201.9823693E-458.15592645
AACGATA150.004637795558.14887244
CTTTCGT201.987131E-458.12771241
GCGAATC258.572237E-658.09952538
TTACGTC150.004691498457.9800429
CGACGTA150.004700493557.95199627
ACGACGT150.004702744557.9449926
CTAAACT258.746176E-657.90298523
ACGGAAC150.00471852357.89599222
AATCGCG150.004736603257.84009619
CTAGATA407.348717E-1057.8400919
TAGACAT150.00474113257.82613811
GAATCGC150.00474113257.82613818
CAACATA150.00474113257.82613810
TGAATCG150.00474113257.82613817
GTATTAG258.815303E-657.82613411
GTCGGTG424250.056.0405731
TCGGTGG420800.056.0328062
Produced by FastQC (version 0.11.5)