FastQCFastQC Report
Fri 17 Jun 2016
SRR1526555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403645
Sequences flagged as poor quality0
Sequence length64
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC11270.2792057377150714No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC9360.23188693034721106No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC8900.22049077778741222No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC7930.1964597604330538No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT5370.13303769401330376No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGC5210.1290738148620694No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG4880.1208983141126485No Hit
GTCGGTGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4480.11098861623456255No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4470.1107408737876104No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC4300.10652925218942388No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC4210.10429957016685454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGA150.004604758758.2536633
GCGAATC150.00460703858.24637638
TTTACAC150.00465281258.10112429
CGATTGT150.004657407858.0866444
AATCGCC150.004657407858.0866451
AAACCGC150.004666609758.0576926
AGCTATA150.00473140357.85584611
ACGATAT150.004759376457.76977510
GACGATA150.004761712657.762619
TCGGTGG406950.056.1802442
GTCGGTG410550.056.144991
CGGTGGG401550.056.1016353
GGTGGGG276050.055.6040464
GGTGGGA89500.055.038324
GTGGGGA83050.054.862625
GTGGGAT21800.054.8343935
GTGGGGG120700.054.6862455
TGGGGAG40600.054.1212466
TTGATCC750.053.98538617
GTCGGTA3850.053.9783331
Produced by FastQC (version 0.11.5)