FastQCFastQC Report
Fri 17 Jun 2016
SRR1526554_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526554_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385075
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC25370.6588326949295592No Hit
GTCGGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC16390.4256313705122379No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC11910.30929039797442054No Hit
GTCGGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT8660.22489125495033435No Hit
GTCGGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC6700.17399207946503928No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA6510.1690579757190158No Hit
GTCGGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT5200.13503862883853796No Hit
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT5140.1334804908134779No Hit
GTCGGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT4540.11789911056287736No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCCCGTTCGCGCC4320.11218593780432384No Hit
GTCGGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCG4250.11036811010842044No Hit
GTCGGTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA4220.1095890410958904No Hit
GTCGGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC4120.10699214438745698No Hit
GTCGGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCCCGTTCGCGCA4090.10621307537492697No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA4010.10413555800818021No Hit
GTCGGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG4010.10413555800818021No Hit
GTCGGTGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGCAGCTCC4000.10387586833733688No Hit
GTCGGTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT3980.1033564889956502No Hit
GTCGGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG3860.1002402129455301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGAC204.1007472E-450.19523640
CCGATGT150.00830221250.18865638
TACGTGA150.008319423550.1623750
CAATCGC150.0083237350.155849
AACCGCG204.216034E-449.91400527
AGATAGG150.00855439149.8101722
ATATTCA150.00859843849.7454918
GAGCGTA204.286982E-449.7454879
CGGTGGG383550.048.480183
TCGGTGG388950.048.402132
GTCGGTG392050.048.330441
GGTGGGG263450.048.2032934
GTGGGGC47450.047.8308375
GTGGGGG113500.047.5568435
GTCGGTA3350.047.5427671
GGTGGGA84750.047.2754334
TGGGGCT24550.047.2371566
GTGGGGA78450.047.1701625
GTGGGAT18800.047.1239285
GTGGGGT28300.047.045355
Produced by FastQC (version 0.11.5)