FastQCFastQC Report
Fri 17 Jun 2016
SRR1526553_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526553_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170008
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3460.20351983436073598No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3280.19293209731306762No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3040.17881511458284316No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2790.16410992423885934No Hit
CTGTGTGGGAGTTCCGACACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCACCTGT2420.1423462425297633No Hit
CTGTGTGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAA2360.1388169968472072No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.12411180650322337No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2020.11881793797938921No Hit
CTGTGTGGGGTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTC1740.10234812479412733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCCG258.481466E-658.17899344
AATCGTG150.004623582658.17899343
GAATCGT150.00462900358.1617942
GCTAGGC201.9809204E-458.1445939
CGAAGGA150.004672534858.0244936
GGCGATG150.00468893757.9731734
TTCGAAG150.00468893757.9731734
TTGGCGA150.004694414357.95608532
ACGCTTG150.004694414357.95608528
GAGGGTA150.004694414357.95608530
CCATACG150.00470538357.92194724
CATACGC150.00470538357.92194725
ATACGCT150.00470538357.92194726
TTCTAGC150.004732887757.8367715
ATATCCC150.004732887757.8367715
CTAGCGC150.004732887757.8367717
CGACACT202.033612E-457.8367615
GTTCCGA202.033612E-457.8367611
TGGGGTT3950.051.9798776
TGGGATC5100.051.5994646
Produced by FastQC (version 0.11.5)