FastQCFastQC Report
Fri 17 Jun 2016
SRR1526552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165814
Sequences flagged as poor quality0
Sequence length64
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2830.17067316390654588No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2630.15861145620997022No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2550.15378677313133995No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.14474049235890818No Hit
CTGTGTGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAA1940.1169985646567841No Hit
CTGTGTGGGAGTTCCGACACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCACCTGT1910.11518930850229775No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.1055399423450372No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA150.00457829258.32306734
TCGACGC150.00457829258.32306733
CATCGCG150.00458382158.30529839
GACGTGC258.417563E-658.25205635
CCGCTCG201.9627553E-458.25205235
GACGCAT150.00460043958.25205235
TTAACCT201.9746041E-458.18121358
CGCCTTA351.5939804E-858.1635357
TTACTAG201.9775753E-458.16352557
ATGCACC258.5091615E-658.14585555
TATCATA150.00463380958.1458552
CCCGTCA150.00463380958.1458553
CTAAAGC150.00463380958.1458554
CGATGGA150.00463380958.1458552
TACGCCG150.00463938858.12818544
CGCCGAC150.00463938858.12818546
CGCTTCG150.00463938858.12818545
GCTCGCC150.004644972358.11053541
TTGACTA150.004650561658.09289628
ACTCGCT150.004650561658.09289628
Produced by FastQC (version 0.11.5)