##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526552_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 165814 Sequences flagged as poor quality 0 Sequence length 64 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.67620948773927 38.0 38.0 40.0 33.0 40.0 2 37.22273149432497 38.0 38.0 40.0 33.0 40.0 3 37.143094069258325 38.0 38.0 40.0 33.0 40.0 4 36.71964972800849 38.0 36.0 40.0 31.0 40.0 5 37.13881216302604 38.0 38.0 40.0 33.0 40.0 6 36.82750551823127 39.0 36.0 40.0 31.0 40.0 7 37.779475798183505 39.0 38.0 40.0 34.0 40.0 8 37.61667289854898 39.0 38.0 40.0 34.0 40.0 9 37.82698083394647 39.0 38.0 40.0 34.0 40.0 10 36.65554175160119 39.0 35.0 40.0 31.0 40.0 11 36.820328802151806 39.0 35.0 40.0 31.0 40.0 12 36.99896872399194 39.0 36.0 40.0 32.0 40.0 13 36.89728852810981 39.0 36.0 40.0 31.0 40.0 14 37.14949280519136 39.0 37.0 40.0 33.0 40.0 15 36.946361585873326 39.0 36.0 40.0 32.0 40.0 16 37.10151133197438 39.0 37.0 40.0 33.0 40.0 17 36.749339621503616 39.0 36.0 40.0 31.0 40.0 18 37.04467053445427 39.0 36.0 40.0 32.0 40.0 19 37.094087350887136 39.0 36.0 40.0 33.0 40.0 20 36.95735583243876 39.0 36.0 40.0 32.0 40.0 21 37.02347208317754 39.0 36.0 40.0 33.0 40.0 22 36.88906847431459 39.0 36.0 40.0 32.0 40.0 23 36.80045110786785 39.0 36.0 40.0 32.0 40.0 24 36.6777111703475 39.0 36.0 40.0 31.0 40.0 25 36.47868696250015 38.0 36.0 40.0 31.0 40.0 26 36.7027150904025 39.0 36.0 40.0 31.0 40.0 27 36.04347039453846 39.0 36.0 40.0 29.0 40.0 28 36.17112547794517 39.0 36.0 40.0 30.0 40.0 29 36.021542209946084 39.0 36.0 40.0 29.0 40.0 30 35.984199162917484 39.0 35.0 40.0 29.0 40.0 31 35.5905231162628 39.0 35.0 40.0 28.0 40.0 32 35.70266684357171 39.0 35.0 40.0 29.0 40.0 33 35.717388157815385 39.0 35.0 40.0 29.0 40.0 34 35.18901902131304 38.0 35.0 40.0 27.0 40.0 35 35.348667784384915 38.0 35.0 40.0 27.0 40.0 36 35.00679677228702 38.0 35.0 40.0 27.0 40.0 37 34.85147816227821 38.0 34.0 40.0 26.0 40.0 38 34.92801572846684 38.0 34.0 40.0 27.0 40.0 39 34.37238110171638 38.0 33.0 40.0 24.0 40.0 40 34.594913577864354 38.0 34.0 40.0 26.0 40.0 41 34.302278456583885 38.0 33.0 40.0 24.0 40.0 42 34.0029973343626 38.0 33.0 40.0 23.0 40.0 43 34.024298310154755 38.0 33.0 39.0 23.0 40.0 44 33.834260074541355 38.0 33.0 39.0 23.0 40.0 45 33.71216543838277 38.0 33.0 39.0 22.0 40.0 46 33.50718877778716 38.0 33.0 39.0 21.0 40.0 47 33.35619428998758 38.0 33.0 39.0 20.0 40.0 48 33.1784228111016 37.0 33.0 39.0 19.0 40.0 49 32.98768499644179 37.0 32.0 39.0 18.0 40.0 50 32.64170094201937 37.0 32.0 39.0 13.0 40.0 51 32.35215964876307 36.0 32.0 39.0 5.0 40.0 52 32.18100401654866 36.0 31.0 39.0 4.0 40.0 53 31.963597766171734 36.0 31.0 39.0 4.0 40.0 54 31.776484494674754 36.0 31.0 39.0 4.0 40.0 55 31.49770827553765 36.0 31.0 39.0 4.0 40.0 56 31.097826480273078 36.0 30.0 39.0 4.0 40.0 57 30.75365771285899 36.0 30.0 39.0 4.0 40.0 58 30.523297188415935 35.0 30.0 39.0 4.0 40.0 59 30.274880287551113 35.0 29.0 39.0 4.0 40.0 60 30.02391836636231 35.0 29.0 39.0 4.0 40.0 61 29.792803985188222 35.0 29.0 39.0 4.0 40.0 62 29.522627763638777 35.0 29.0 39.0 4.0 40.0 63 29.287834561617235 35.0 28.0 38.0 4.0 40.0 64 29.17496110099268 35.0 27.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.019458005328154115 2 2 -0.05431142392623656 2 3 -0.2683366803488667 2 4 0.0942746953100766 2 5 -0.23091760547251994 2 6 0.005711360161875234 2 7 0.015288440748484788 2 8 0.2023807321709299 2 9 -0.2628443542123051 2 10 0.11044884108756747 2 11 -0.49093029127544696 2 12 -0.08567309378385346 2 13 -0.07872644331097689 2 14 -0.1850464343762397 2 15 0.11742443975246886 2 16 0.006593756612346624 2 17 0.7077057174333135 2 18 0.06766279199764114 2 19 -0.10797211744056767 2 20 -0.07882469597176112 2 21 0.003591129700865281 2 22 -0.6165022713043271 2 23 -0.3358700881407657 2 24 -0.5483596028816393 2 25 0.13599538331290972 2 26 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-0.031427397185595396 119 10 -0.10732119375563798 119 11 -0.13944945503850903 119 12 0.07329903165168616 119 13 0.13904359153222856 119 14 -0.17096397212304026 119 15 0.24939308086744916 119 16 0.6051709923847071 119 17 0.7296279009408622 119 18 0.34147231580716664 119 19 0.4252741078788276 119 20 0.9249499846321854 119 21 0.7123600031038819 119 22 0.795808994665478 119 23 0.3246990175502873 119 24 1.0248111288256752 119 25 0.3608792392943272 119 26 0.5131378646423315 119 27 1.1138828388473243 119 28 0.8795922022943117 119 29 0.7993120338553155 119 30 0.4698878526717891 119 31 0.8742785852262429 119 32 0.8869250756685148 119 33 0.3194812066255466 119 34 0.6979958739750742 119 35 1.0142803679175927 119 36 0.9856649136866196 119 37 0.5352297008949094 119 38 0.42460237919271293 119 39 0.7564111143526944 119 40 0.17356047752918613 119 41 0.3207978839614256 119 42 -0.029782320704441645 119 43 0.0313411556436165 119 44 -0.19772174216579685 119 45 -0.3605979880505572 119 46 -0.3719905691092791 119 47 -0.10025107398043076 119 48 -0.6938234116431587 119 49 -0.7052488123900105 119 50 -0.4262932252508804 119 51 -0.6676191115540924 119 52 -0.820929180021011 119 53 -0.1141594056870936 119 54 -0.5925370332664741 119 55 -0.15131796957539123 119 56 -0.8683403941933889 119 57 -0.6488654863437127 119 58 -0.8664055207901988 119 59 -1.1244015477868814 119 60 -0.5912717672607641 119 61 -1.240827591736256 119 62 -0.9983276690164367 119 63 -1.2805096307363364 119 64 -0.9977953601847958 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 423.0 5 27.0 6 49.0 7 123.0 8 166.0 9 110.0 10 176.0 11 237.0 12 330.0 13 434.0 14 523.0 15 510.0 16 660.0 17 739.0 18 765.0 19 883.0 20 979.0 21 1099.0 22 1359.0 23 1400.0 24 1701.0 25 1935.0 26 1974.0 27 2361.0 28 2858.0 29 3373.0 30 4178.0 31 4840.0 32 5923.0 33 7315.0 34 9251.0 35 11882.0 36 16516.0 37 22778.0 38 28890.0 39 29004.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.55552339080772 0.23761707465639004 0.10433440080090221 0.10252513373499063 8 99.03687745532885 0.45802632293571344 0.3819903325669388 0.12310588916849213 9 98.36503552172917 0.8400979410664962 0.37692836551799 0.41793817168634734 10 47.52915917835647 32.10645663212998 8.618090149203324 11.746294040310227 11 29.588575150469804 30.332782515348523 23.826697383815603 16.251944950366074 12 39.18788522078956 16.931019093683283 23.86831027536879 20.012785410158372 13 26.18415815311132 17.459924976178126 31.84592374588394 24.50999312482661 14 28.852207895593857 21.91190128698421 26.929571688759697 22.306319128662235 15 25.308768667978605 22.657523331200423 24.719603037583454 27.31410496323751 16 28.02963123827449 28.450090787894144 25.323488426805653 18.196789547025716 17 35.57217235839142 21.663183580773364 21.808144380956985 20.95649967987823 18 25.412811945915305 21.861845200043422 31.10292255177488 21.622420302266395 19 32.72884074927328 22.633191407239437 23.251353926688942 21.386613916798343 20 23.16934880691003 23.03725239462446 26.052549038531136 27.74084975993437 21 26.05630405152762 21.06034472360597 32.30065012604484 20.58270109882157 22 25.486388362864414 21.623626473036055 26.962741384925277 25.92724377917426 23 26.52067979784578 23.027006163532633 31.942417407456546 18.50989663116504 24 32.170750683014795 24.091575246217033 24.5010825709099 19.23659149985827 25 26.693337677932455 23.03844366804849 30.143673479239453 20.124545174779602 26 32.02910091211814 23.224627189807443 24.02695333236813 20.71931856570629 27 26.155333353472493 21.784128326737758 29.59731746367399 22.463220856115758 28 31.45635759589248 23.014195107218363 24.190878888553307 21.33856840833585 29 31.199913036651427 24.38416059231703 24.021813306600155 20.394113064431387 30 30.767975732232745 23.809120958141726 25.795983974572025 19.626919335053508 31 27.566008994632234 25.71509744184922 26.22164998307462 20.49724358044393 32 26.557091857999747 26.33359307516204 27.5549837208319 19.554331346006318 33 26.81225558047182 25.459523823915188 27.940740919833416 19.787479675779572 34 23.196565983132633 26.583964497399666 31.083543721355444 19.13592579811226 35 23.143384020073157 29.30136944889507 28.864838719429248 18.690407811602526 36 23.23599721608618 29.395103942869248 27.37495082760916 19.99394801343541 37 22.931231727469175 29.67670861404178 27.55633841181064 19.83572124667841 38 24.90407311021001 28.79622344610543 26.76329964292199 19.53640380076257 39 23.698719972796386 27.963250831896236 27.95596414952272 20.382065045784653 40 24.390081483902996 27.501119949632535 27.560447011247923 20.54835155521654 41 21.618380892398626 28.227799040744152 27.85293832663146 22.300881740225766 42 20.863265739140978 27.569337091934166 29.118244369676578 22.449152799248278 43 22.30082380112386 27.044802899973934 29.521055726296773 21.13331757260543 44 22.81427801038555 27.074534801286987 30.233826354090294 19.87736083423717 45 24.207176627469995 27.807613044005336 27.779730876469877 20.205479452054796 46 23.045858876175576 28.07378167464028 28.501264256998894 20.379095192185254 47 22.6391642565679 28.3621635714329 29.12490677919857 19.873765392800628 48 23.276734782951326 29.047119657135234 28.45486563653668 19.22127992337676 49 24.864953347520053 28.196407243714493 27.805969334861135 19.13267007390432 50 22.729976811101533 28.923598042905535 27.783578374834583 20.562846771158352 51 21.96693071949341 29.746582072713597 28.748802057428456 19.53768515036454 52 21.63868987314895 29.878244832836888 28.276682095863237 20.206383198150924 53 21.77058173829666 29.41401366654125 28.850238496923208 19.965166098238885 54 21.820697019706824 29.04693719056402 28.196291622172602 20.936074167556548 55 21.53410056571248 30.193386262655693 27.954208852307765 20.31830431932406 56 21.675044316553752 29.74733493601418 26.956606201889223 21.621014545542845 57 21.697672301295864 30.69891657309338 27.14697581257018 20.456435313040576 58 21.881035383288594 32.56768739295735 26.60488053736927 18.946396686384784 59 22.729978256380356 31.82767877749839 26.41120950402682 19.03113346209443 60 23.106741846108978 30.571015408545183 26.424196762747293 19.898045982598553 61 23.341568082355582 29.5613326939769 26.2156371207109 20.88146210295662 62 21.70478624902095 29.803704895537976 27.076338334314908 21.41517052112616 63 20.706110925594245 30.665735709298442 26.729607480040073 21.898545885067243 64 21.67421226945398 30.0647200024308 27.040199325453496 21.220868402661726 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 5.5 8 9.0 9 73.5 10 238.5 11 339.0 12 327.5 13 278.5 14 241.0 15 329.0 16 504.0 17 591.0 18 620.5 19 650.0 20 794.5 21 1070.5 22 1202.0 23 1530.5 24 2059.0 25 2259.0 26 2407.5 27 2656.5 28 2757.0 29 2851.5 30 3035.0 31 3124.0 32 3137.0 33 3150.0 34 3400.5 35 3764.0 36 3877.0 37 4234.5 38 4825.0 39 5058.0 40 5109.5 41 5334.5 42 5508.0 43 5845.0 44 6182.0 45 6198.0 46 6417.0 47 6620.0 48 6556.5 49 6448.5 50 6404.0 51 6355.5 52 6301.5 53 6296.0 54 6891.0 55 7773.5 56 8061.0 57 8422.5 58 8784.0 59 9108.0 60 9572.0 61 9712.0 62 8968.5 63 6954.0 64 5683.0 65 5032.0 66 3703.5 67 3026.0 68 2396.5 69 1767.0 70 1518.5 71 1034.5 72 799.0 73 672.0 74 428.5 75 312.0 76 250.5 77 156.0 78 123.0 79 97.0 80 53.5 81 36.0 82 31.5 83 27.0 84 18.0 85 7.5 86 6.0 87 5.5 88 5.0 89 5.0 90 3.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 6.030853848287841E-4 8 0.06211779463736476 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.09408132003329031 16 0.025932671547637714 17 0.1519775169768536 18 0.0 19 0.0 20 0.015680220005548386 21 0.0 22 0.0 23 0.0 24 0.00301542692414392 25 0.05488077001941935 26 0.027741927702124067 27 0.1803225300638064 28 0.159214541594799 29 0.13690038235613397 30 0.1972089208390124 31 0.2303786170045955 32 0.15981762697962779 33 0.22374467777147888 34 0.3877839024449081 35 0.25269277624326053 36 0.34918643781586595 37 0.36607282859107193 38 0.35220186474000986 39 0.681486484856526 40 0.3787376216724764 41 0.41371657399254586 42 0.5180503455679255 43 0.5108133209499801 44 0.4685973440119652 45 0.5029732109472059 46 0.5397614194217617 47 0.5325243948038163 48 0.5132256624892952 49 0.5252873701858709 50 0.6513322156150868 51 0.5717249448176873 52 0.5880082502080645 53 0.6223841171433052 54 0.6018792140591265 55 0.6428890202274838 56 0.6573630694633746 57 0.6386674225336824 58 0.6995790464013895 59 0.7044037294800197 60 0.7417950233394044 61 0.6422859348426551 62 0.6712340333144367 63 0.6688216917751215 64 0.7592844994994391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 165814.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.753519003220475 #Duplication Level Percentage of deduplicated Percentage of total 1 75.12804414631728 41.135247928401704 2 10.125676018019805 11.088327885462023 3 5.119562942647237 8.409422606052566 4 2.975030014649352 6.5157344976901825 5 1.9550826641994072 5.352382790355459 6 1.3184416614347554 4.331359233840327 7 0.8635407373139917 3.309732591940367 8 0.6201191774333895 2.7162965732688433 9 0.4152485433257333 2.0462687107240645 >10 1.42528279857692 12.088846538892977 >50 0.037449470750861885 1.4184568251173 >100 0.016521825331262598 1.5879238182541884 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 283 0.17067316390654588 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 263 0.15861145620997022 No Hit CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 255 0.15378677313133995 No Hit CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 240 0.14474049235890818 No Hit CTGTGTGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAA 194 0.1169985646567841 No Hit CTGTGTGGGAGTTCCGACACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAATGCACCTGT 191 0.11518930850229775 No Hit CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 175 0.1055399423450372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.001206170769657568 0.0 0.0 0.0 0.0 9 0.0018092561544863523 0.0 0.0 0.0 0.0 10 0.004221597693801488 0.0 0.0 0.0 0.0 11 0.007237024617945409 0.0 0.0 0.0 0.0 12 0.007840110002774193 0.0 0.0 0.0 0.0 13 0.009649366157260544 0.0 0.0 0.0 0.0 14 0.01206170769657568 0.0 0.0 0.0 0.0 15 0.015680220005548386 0.0 0.0 0.0 0.0 16 0.01748947616003474 0.0 0.0 0.0 0.0 17 0.018695646929692306 0.0 0.0 0.0 0.0 18 0.019901817699349875 0.0 0.0 0.0 0.0 19 0.021711073853836225 0.0 0.0 0.0 0.0 20 0.024726500777980148 0.0 0.0 0.0 0.0 21 0.025932671547637714 0.0 0.0 0.0 0.0 22 0.028948098471781637 0.0 0.0 0.0 0.0 23 0.03316969616558312 0.0 0.0 0.0 0.0 24 0.037994379244213394 0.0 0.0 0.0 0.0 25 0.04342214770767245 0.0 0.0 0.0 0.0 26 0.04764374540147394 0.0 0.0 0.0 0.0 27 0.05246842848010421 0.0 0.0 0.0 0.0 28 0.060308538482878406 0.0 0.0 0.0 0.0 29 0.06513322156150868 0.0 0.0 0.0 0.0 30 0.0681486484856526 0.0 0.0 0.0 0.0 31 0.07116407540979652 0.0 0.0 0.0 0.0 32 0.08021035618222828 0.0 0.0 0.0 0.0 33 0.08382886849120098 0.0 0.0 0.0 0.0 34 0.0868442954153449 0.0 0.0 0.0 0.0 35 0.09589057618777666 0.0 0.0 0.0 0.0 36 0.09830291772709181 0.0 0.0 0.0 0.0 37 0.1019214300360645 0.0 0.0 0.0 0.0 38 0.11217388157815383 0.0 0.0 0.0 0.0 39 0.12061707696575681 0.0 0.0 0.0 6.03085384828784E-4 40 0.128457186968531 0.0 0.0 0.0 6.03085384828784E-4 41 0.13810655312579154 0.0 0.0 0.0 6.03085384828784E-4 42 0.1441374069740794 0.0 0.0 0.0 6.03085384828784E-4 43 0.15378677313133993 0.0 0.0 0.0 6.03085384828784E-4 44 0.16102379774928535 0.0 0.0 0.0 6.03085384828784E-4 45 0.16826082236723075 0.0 0.0 0.0 6.03085384828784E-4 46 0.17670401775483374 0.0 0.0 0.0 6.03085384828784E-4 47 0.18695646929692306 0.0 0.0 0.0 6.03085384828784E-4 48 0.19841509160866996 0.0 0.0 0.0 6.03085384828784E-4 49 0.20504903084178658 0.0 0.0 0.0 6.03085384828784E-4 50 0.21288914084456076 0.0 0.0 0.0 6.03085384828784E-4 51 0.21891999469284862 0.0 0.0 0.0 6.03085384828784E-4 52 0.22856936085010915 0.0 0.0 0.0 6.03085384828784E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGCA 15 0.004578292 58.323067 34 TCGACGC 15 0.004578292 58.323067 33 CATCGCG 15 0.004583821 58.305298 39 GACGTGC 25 8.417563E-6 58.252056 35 CCGCTCG 20 1.9627553E-4 58.252052 35 GACGCAT 15 0.004600439 58.252052 35 TTAACCT 20 1.9746041E-4 58.181213 58 CGCCTTA 35 1.5939804E-8 58.16353 57 TTACTAG 20 1.9775753E-4 58.163525 57 ATGCACC 25 8.5091615E-6 58.145855 55 TATCATA 15 0.004633809 58.14585 52 CCCGTCA 15 0.004633809 58.14585 53 CTAAAGC 15 0.004633809 58.14585 54 CGATGGA 15 0.004633809 58.14585 52 TACGCCG 15 0.004639388 58.128185 44 CGCCGAC 15 0.004639388 58.128185 46 CGCTTCG 15 0.004639388 58.128185 45 GCTCGCC 15 0.0046449723 58.110535 41 TTGACTA 15 0.0046505616 58.092896 28 ACTCGCT 15 0.0046505616 58.092896 28 >>END_MODULE