FastQCFastQC Report
Fri 17 Jun 2016
SRR1526551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146468
Sequences flagged as poor quality0
Sequence length56
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3550.24237376082147638No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3550.24237376082147638No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3110.21233306933937787No Hit
CTGTGTGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC2620.17887866291613183No Hit
CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT2600.17751317693967283No Hit
CTGTGTGGGAGTTCCGACACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAAT1770.12084550891662343No Hit
CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1750.11948002294016441No Hit
CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1650.1126525930578693No Hit
CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.11060436409318075No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGT150.00797141850.691437
GCGACAT150.00797141850.691435
CAGTTGC150.00797141850.691435
AGTTGCT150.00797141850.691436
CGACGCA150.00798240550.67374834
ATCGACG150.00808178950.5154532
TAAGCAA150.00820450750.32330744
CGGGTCA150.00821573150.30591243
CGTACTG355.0551535E-850.23645442
GCCGGAT150.00827202550.2191250
GCTAGGA150.00827202550.2191249
CTACGTA355.0826202E-850.20179739
TACGTAC355.0826202E-850.20179740
ATCGCGT150.00828331850.20179440
ACGTACT355.1803E-850.08086841
CTATCGA150.00844262849.9605230
GAATACG150.00844262849.9605230
CCTATCG150.00845409449.9433829
ATAGCTC204.2667202E-449.7725722
TGACGAT150.0085925949.73855220