##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526551_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 146468 Sequences flagged as poor quality 0 Sequence length 56 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.682579129912334 38.0 38.0 40.0 33.0 40.0 2 37.19004151077368 38.0 38.0 40.0 33.0 40.0 3 37.135674686620966 38.0 38.0 40.0 33.0 40.0 4 36.53041620012562 38.0 36.0 40.0 30.0 40.0 5 36.984843105661305 38.0 38.0 40.0 33.0 40.0 6 36.5815604773739 39.0 36.0 40.0 31.0 40.0 7 37.73606521561024 39.0 38.0 40.0 35.0 40.0 8 37.92804571647049 39.0 38.0 40.0 35.0 40.0 9 37.92571073545075 39.0 38.0 40.0 35.0 40.0 10 36.617022148182535 39.0 35.0 40.0 30.0 40.0 11 37.0163107299888 39.0 36.0 40.0 32.0 40.0 12 37.24044842559467 39.0 36.0 40.0 32.0 40.0 13 37.176161345822976 39.0 36.0 40.0 32.0 40.0 14 37.45813419996177 39.0 37.0 40.0 33.0 40.0 15 37.47829560040418 39.0 38.0 40.0 33.0 40.0 16 37.67812764562908 39.0 38.0 40.0 33.0 40.0 17 37.5866878772155 39.0 38.0 40.0 33.0 40.0 18 37.47269028047082 39.0 38.0 40.0 33.0 40.0 19 37.526872764016716 39.0 38.0 40.0 33.0 40.0 20 37.41885599584892 39.0 38.0 40.0 33.0 40.0 21 37.32476035721113 39.0 37.0 40.0 33.0 40.0 22 37.20706912090013 39.0 37.0 40.0 33.0 40.0 23 37.07441898571702 39.0 36.0 40.0 33.0 40.0 24 36.96438129830407 39.0 36.0 40.0 32.0 40.0 25 36.74890761121883 39.0 36.0 40.0 32.0 40.0 26 36.39259770052161 38.0 36.0 40.0 31.0 40.0 27 36.131148100609 38.0 35.0 40.0 30.0 40.0 28 36.18576071223749 38.0 35.0 40.0 30.0 40.0 29 36.03484720211923 38.0 35.0 40.0 30.0 40.0 30 35.82521096758336 38.0 35.0 40.0 29.0 40.0 31 35.15632083458503 38.0 35.0 40.0 28.0 40.0 32 34.943625911461886 38.0 34.0 40.0 27.0 40.0 33 34.91085424802687 38.0 34.0 39.0 27.0 40.0 34 35.135490346014144 38.0 35.0 40.0 28.0 40.0 35 35.013170112242946 38.0 34.0 40.0 27.0 40.0 36 34.668262009449165 38.0 34.0 40.0 26.0 40.0 37 34.18956359068192 38.0 34.0 40.0 23.0 40.0 38 34.351394161181965 38.0 33.0 40.0 25.0 40.0 39 34.17863287544037 38.0 33.0 39.0 24.0 40.0 40 33.856719556490155 38.0 33.0 39.0 23.0 40.0 41 33.39650981784417 38.0 33.0 39.0 21.0 40.0 42 33.29663134609608 37.0 33.0 39.0 20.0 40.0 43 33.180817653002705 37.0 32.0 39.0 19.0 40.0 44 32.70218750853429 37.0 32.0 39.0 15.0 40.0 45 32.66828931916869 37.0 32.0 39.0 15.0 40.0 46 32.179650162492834 36.0 32.0 39.0 4.0 40.0 47 31.94121582871344 36.0 31.0 39.0 4.0 40.0 48 31.558231149466096 36.0 31.0 39.0 4.0 40.0 49 31.47185733402518 36.0 31.0 39.0 4.0 40.0 50 31.195312286642817 36.0 30.0 39.0 4.0 40.0 51 30.59983067973892 36.0 30.0 39.0 4.0 40.0 52 30.28639020127263 35.0 30.0 39.0 4.0 40.0 53 29.991629570964307 35.0 29.0 39.0 4.0 40.0 54 29.727346587650544 35.0 29.0 38.0 4.0 40.0 55 29.265102274899636 35.0 28.0 38.0 4.0 40.0 56 17.329355217521915 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.5107010714139193 1 2 0.028627592541894842 1 3 -0.5027780289218953 1 4 -0.024925844921476425 1 5 -0.8563413475755866 1 6 0.8893250582199599 1 7 -0.7833535698940537 1 8 -1.3936600790625775 1 9 -1.2055131321495196 1 10 0.15052743569621185 1 11 -0.9384985886658086 1 12 -0.7650366892664593 1 13 -0.5707350579957691 1 14 -1.0182634599073594 1 15 -0.5974608105912722 1 16 -0.43764624343774017 1 17 -0.6692719899055533 1 18 -1.332014552578002 1 19 -0.5473006625088317 1 20 -1.141091398130115 1 21 -0.7802430801823732 1 22 -0.7433830130428518 1 23 -0.9664384098916514 1 24 -1.0909754116164265 1 25 -0.6264005266256234 1 26 -1.529566333655879 1 27 -1.2528670737561 1 28 -1.099364599486286 1 29 -1.0523308844131734 1 30 -0.9645759119915951 1 31 -0.4210404241765815 1 32 -0.46020112939363145 1 33 -0.7324884952642208 1 34 -0.42954359979285783 1 35 -0.6554308593039053 1 36 -0.6091703041449179 1 37 -0.4526800776167761 1 38 -0.7966817091610707 1 39 -0.4662793611828491 1 40 0.32120348592164305 1 41 0.43859738621220856 1 42 1.076018387024412 1 43 -0.2957110736464017 1 44 1.0231511574457386 1 45 1.0064715385035257 1 46 0.535749981785937 1 47 0.7142426371408916 1 48 0.7660291031356579 1 49 0.49333365442013744 1 50 0.8249506123922927 1 51 0.6411211072310934 1 52 1.0724891529816603 1 53 1.0251605394794403 1 54 0.5916328681023799 1 55 1.3832944313169016 1 56 2.0745183239925318 2 1 -0.0705182415252068 2 2 0.45072616169928637 2 3 -0.48250775865162154 2 4 0.26382614235992463 2 5 0.36921509121614093 2 6 -0.048671762129799845 2 7 0.23592305967669347 2 8 0.004789841446161347 2 9 0.10311166912234171 2 10 0.02473252313659202 2 11 -0.5297148048820262 2 12 -0.7157521105701221 2 13 -0.2557509562469562 2 14 -0.4892491514812889 2 15 -0.39296955462942407 2 16 -0.9638783579051449 2 17 -0.7074277927672341 2 18 -0.7153611344539925 2 19 -1.0379604399333147 2 20 -1.0910119068741508 2 21 -1.3776198687356285 2 22 -1.2223178302129583 2 23 -1.4020504925625517 2 24 -0.6150215165448856 2 25 -0.9016390003935086 2 26 -0.9141052843713027 2 27 -1.1062057065064934 2 28 -1.6265903546532172 2 29 -1.2830542548424262 2 30 -1.3725647832157577 2 31 -0.8208814416646604 2 32 -1.0653283154031712 2 33 -0.7245393696680367 2 34 -0.8669442357229045 2 35 -0.7239920675709968 2 36 -0.7184707810924564 2 37 -0.6460425577439679 2 38 -0.20526676480494643 2 39 -0.6193000289094002 2 40 -1.0788362597063426 2 41 -0.05623568215027319 2 42 -0.6183377338022993 2 43 -0.5564423932012517 2 44 0.5287155453630703 2 45 -0.16026137087644088 2 46 -0.7227953282299637 2 47 -0.6384998111897957 2 48 0.12195120170481744 2 49 -0.440887331271437 2 50 -0.5035470028700288 2 51 -0.45824296272121146 2 52 0.01764018636798781 2 53 0.1696358971900942 2 54 0.263731437259775 2 55 -0.21308871653683426 2 56 -0.8650683214764676 3 1 -0.2742677656972674 3 2 0.20281980463799698 3 3 -0.041510423288094955 3 4 -0.1807874853522975 3 5 0.338666937428556 3 6 -0.0255465242158337 3 7 0.33232580044563065 3 8 0.21187016120254043 3 9 0.08912232766821404 3 10 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0.33195184345486695 120 35 0.058840060331554866 120 36 0.48527874307960417 120 37 0.6930324327394786 120 38 -0.03777532970787689 120 39 0.29552676972021885 120 40 0.2113236184817069 120 41 0.018754801290917555 120 42 0.654311676171055 120 43 0.6564430274306474 120 44 0.8253466835435006 120 45 0.5956388955872001 120 46 0.8939728483973717 120 47 0.41422192463053165 120 48 1.1134814643618398 120 49 -0.07449981699659958 120 50 -0.10328053922328095 120 51 -0.17122354894123504 120 52 -0.01947439300011311 120 53 -0.10729596424880938 120 54 -0.19192139867475078 120 55 -0.5612996034801156 120 56 -0.34822401237863687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 357.0 5 27.0 6 29.0 7 55.0 8 114.0 9 139.0 10 88.0 11 190.0 12 200.0 13 295.0 14 365.0 15 442.0 16 502.0 17 509.0 18 707.0 19 773.0 20 784.0 21 934.0 22 1008.0 23 1058.0 24 1502.0 25 1958.0 26 1959.0 27 2171.0 28 2535.0 29 2823.0 30 3425.0 31 4214.0 32 5052.0 33 5949.0 34 7982.0 35 11071.0 36 16044.0 37 23436.0 38 28707.0 39 19064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 100.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.45143528575919 0.2869205230151248 0.17966689893566148 0.08197729229003566 8 99.28926454925308 0.438320998443346 0.21301581232760738 0.05939863997596745 9 98.34776196848458 0.8704973099926263 0.4041838490318705 0.3775568724909195 10 47.301799710516974 32.306032717044 8.538383810798262 11.853783761640768 11 29.88024687986454 30.18406750962668 23.736242728787175 16.199442881721605 12 38.422044405603955 16.867848267198298 24.04962176038452 20.66048556681323 13 25.994073790862167 17.663926591473906 31.929158587541306 24.41284103012262 14 28.752355463309392 21.653193871698935 27.25646557609853 22.337985088893138 15 25.047792009176067 22.741486194936776 24.84570008466013 27.365021711227026 16 27.885954611246145 28.228691591337356 25.26900073736243 18.616353060054074 17 35.15375372094929 21.527569161864708 21.990468907884317 21.328208209301692 18 25.518201928066198 21.937214954802414 30.9180162219734 21.626566895157985 19 32.63033563645301 22.492285004233008 23.370292487096158 21.507086872217823 20 23.347079225496355 23.05554796952235 26.29516344867138 27.30220935630991 21 26.06371357566158 21.075593303656774 31.922331157659013 20.93836196302264 22 25.357074582844035 21.667531474451756 27.30357484228637 25.671819100417835 23 26.69183712483273 23.062375399404647 31.49493404702734 18.750853428735287 24 31.90662806892973 24.128819947019146 24.45653658136931 19.508015402681814 25 26.55970383727443 23.50655027799407 29.948909197710478 19.984836687021023 26 31.345638867635806 23.174663897693737 24.494480271067875 20.98521696360258 27 26.424909426481648 21.714402898352585 29.70537972520336 22.155307949962403 28 31.116837665844137 23.234242670731454 24.186415305850428 21.462504357573977 29 31.264275065990127 24.179739321908723 24.278895468906 20.277090143195153 30 30.917315667729707 23.669947030563502 25.69496721917302 19.71777008253377 31 27.420425537739273 25.668452857563253 26.120817678499325 20.79030392619815 32 26.912092051642066 25.927879182219787 27.204547789459266 19.95548097667888 33 27.114417636017375 25.321632619507582 27.673421294203077 19.890528450271965 34 23.379586802343507 26.726282248946447 30.951450988453765 18.942679960256278 35 23.509132654809637 28.7536410678181 28.893458072033173 18.84376820533909 36 23.419235858305395 29.202745041580116 27.023302688138873 20.35471641197562 37 23.293948266434093 29.286846495144353 27.480255553017564 19.93894968540399 38 24.867412677612126 28.741775469458602 26.726036513828188 19.66477533910108 39 24.06789894748398 27.75376339659407 27.93009756700012 20.248240088921822 40 24.597231676877694 27.372430912253918 27.1668351314369 20.863502279431483 41 21.826832634173467 28.191361542316905 27.694969481843586 22.28683634166604 42 21.289835164835164 27.03365384615385 28.921016483516482 22.755494505494507 43 22.32812231575331 26.644906373475347 29.304243257172306 21.722728053599038 44 23.10283200439923 26.777563926312897 29.626752818256808 20.49285125103107 45 24.383252795897747 27.352025350389397 27.622163718973614 20.642558134739243 46 23.173088163495457 27.8855476781527 28.205992174552858 20.735371983798988 47 22.94628860592393 28.190231291768654 28.536068864992714 20.327411237314706 48 23.538482720638854 28.470329065124606 28.015971361696266 19.975216852540274 49 24.841689380798986 27.932187990418768 27.511632388976075 19.714490239806175 50 22.97474017482277 28.619313097942044 27.571753045632875 20.83419368160231 51 22.37894070537078 29.3046744918881 28.602881479544802 19.713503323196324 52 21.949691595990746 29.440191651062893 28.0861052979403 20.524011455006058 53 22.21028639108669 29.067421378450774 28.57231392154027 20.149978308922265 54 22.275096930588745 28.807839842431836 27.83336891472174 21.083694312257673 55 22.086016321752005 29.601597741124618 27.676732894872767 20.635653042250613 56 21.905988320846188 29.669595636844427 26.54390700749229 21.8805090348171 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.5 7 2.0 8 8.5 9 15.0 10 71.5 11 128.0 12 212.0 13 292.0 14 288.0 15 247.5 16 207.0 17 304.0 18 401.0 19 518.5 20 636.0 21 659.0 22 850.0 23 1018.0 24 1201.0 25 1384.0 26 1754.0 27 2124.0 28 2534.5 29 3254.0 30 3563.0 31 3626.0 32 3689.0 33 3728.5 34 3768.0 35 3947.5 36 4127.0 37 4034.5 38 4185.0 39 4428.0 40 4688.0 41 4948.0 42 5150.5 43 5353.0 44 5695.5 45 6038.0 46 6184.5 47 6235.5 48 6140.0 49 6223.0 50 6306.0 51 6461.5 52 6617.0 53 6734.5 54 7351.0 55 7850.0 56 8375.5 57 8901.0 58 9334.5 59 9768.0 60 9936.0 61 10104.0 62 9594.0 63 7723.5 64 6363.0 65 5446.5 66 4530.0 67 3691.5 68 2853.0 69 2335.5 70 1818.0 71 1422.5 72 900.0 73 773.0 74 644.0 75 515.0 76 411.0 77 307.0 78 240.5 79 130.5 80 87.0 81 62.0 82 37.0 83 31.5 84 26.0 85 19.5 86 13.0 87 10.0 88 3.5 89 0.0 90 1.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.05871589698773793 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.04233006527022968 26 0.05735041101127892 27 0.12152825190485295 28 0.11674905098724636 29 0.15976185924570555 30 0.23622907392741077 31 0.23691181691564028 32 0.31611000355026353 33 0.33727503618537835 34 0.36390201272632927 35 0.38438430237321464 36 0.4130595078788541 37 1.3634377474943333 38 0.48816123658410027 39 0.49294043750170685 40 0.7080044787940029 41 0.5578010213835104 42 0.5926209137832155 43 0.6438266379004288 44 0.6745500723707567 45 0.6738673293825272 46 0.7134664226998388 47 0.6991288199470191 48 0.8247535297812492 49 0.808367698063741 50 0.8042712401343638 51 0.7687686047464293 52 0.8220225578283311 53 0.8513805063222002 54 0.8609389081574131 55 0.8623043941338723 56 0.8547942212633476 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 146468.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.21623835923205 #Duplication Level Percentage of deduplicated Percentage of total 1 65.83643562016192 30.42712401343638 2 13.77267623943745 12.730425758527462 3 7.080600366365301 9.817161427752136 4 4.173314424156473 7.714995766993472 5 2.654671157596171 6.134445749242155 6 1.7136441529279678 4.751891198077396 7 1.1803462743012467 3.8185815331676545 8 0.8597766353483425 3.1788513531966025 9 0.6130709685044023 2.550045061037223 >10 2.022395556343438 14.476199579430318 >50 0.06352301601370915 2.0065816424065326 >100 0.029545588843585654 2.393696916732665 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 355 0.24237376082147638 No Hit CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 355 0.24237376082147638 No Hit CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 311 0.21233306933937787 No Hit CTGTGTGGGATTGCTTCTGTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTC 262 0.17887866291613183 No Hit CTGTGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 260 0.17751317693967283 No Hit CTGTGTGGGAGTTCCGACACTTGTGAAACTCCGAGACGTGCCGTGGTCTCAGCAAT 177 0.12084550891662343 No Hit CTGTGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 175 0.11948002294016441 No Hit CTGTGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 165 0.1126525930578693 No Hit CTGTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 162 0.11060436409318075 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.827429882295109E-4 0.0 0.0 0.0 0.0 8 0.0027309719529180435 0.0 0.0 0.0 0.0 9 0.004096457929377065 0.0 0.0 0.0 0.0 10 0.004779200917606577 0.0 0.0 0.0 0.0 11 0.011606630799901685 0.0 0.0 0.0 0.0 12 0.014337602752819728 0.0 0.0 0.0 0.0 13 0.014337602752819728 0.0 0.0 0.0 0.0 14 0.01502034574104924 0.0 0.0 0.0 0.0 15 0.01638583171750826 0.0 0.0 0.0 0.0 16 0.021847775623344348 0.0 0.0 0.0 0.0 17 0.02389600458803288 0.0 0.0 0.0 0.0 18 0.028675205505639456 0.0 0.0 0.0 0.0 19 0.03072343447032799 0.0 0.0 0.0 0.0 20 0.03481989239970505 0.0 0.0 0.0 0.0 21 0.03891635032908212 0.0 0.0 0.0 0.0 22 0.04779200917606576 0.0 0.0 0.0 0.0 23 0.05188846710544283 0.0 0.0 0.0 0.0 24 0.05530218204659038 0.0 0.0 0.0 0.0 25 0.05530218204659038 0.0 0.0 0.0 0.0 26 0.058033153999508424 0.0 0.0 0.0 0.0 27 0.062812354917115 0.0 0.0 0.0 0.0 28 0.0682742988229511 0.0 0.0 0.0 0.0 29 0.07373624272878718 0.0 0.0 0.0 0.0 30 0.07714995766993472 0.0 0.0 0.0 0.0 31 0.0812464155993118 0.0 0.0 0.0 0.0 32 0.08397738755222983 0.0 0.0 0.0 0.0 33 0.08875658846983642 0.0 0.0 0.0 0.0 34 0.0942185323756725 0.0 0.0 0.0 0.0 35 0.09831499030504957 0.0 0.0 0.0 0.0 36 0.10377693421088566 0.0 0.0 0.0 0.0 37 0.10855613512849223 0.0 0.0 0.0 0.0 38 0.1133353360460988 0.0 0.0 0.0 0.0 39 0.11538356501078734 0.0 0.0 0.0 6.827429882295109E-4 40 0.12289373788131196 0.0 0.0 0.0 6.827429882295109E-4 41 0.12835568178714804 0.0 0.0 0.0 6.827429882295109E-4 42 0.1372313406341317 0.0 0.0 0.0 6.827429882295109E-4 43 0.14883797143403338 0.0 0.0 0.0 6.827429882295109E-4 44 0.15498265832809896 0.0 0.0 0.0 6.827429882295109E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGT 15 0.007971418 50.6914 37 GCGACAT 15 0.007971418 50.6914 35 CAGTTGC 15 0.007971418 50.6914 35 AGTTGCT 15 0.007971418 50.6914 36 CGACGCA 15 0.007982405 50.673748 34 ATCGACG 15 0.008081789 50.51545 32 TAAGCAA 15 0.008204507 50.323307 44 CGGGTCA 15 0.008215731 50.305912 43 CGTACTG 35 5.0551535E-8 50.236454 42 GCCGGAT 15 0.008272025 50.21912 50 GCTAGGA 15 0.008272025 50.21912 49 CTACGTA 35 5.0826202E-8 50.201797 39 TACGTAC 35 5.0826202E-8 50.201797 40 ATCGCGT 15 0.008283318 50.201794 40 ACGTACT 35 5.1803E-8 50.080868 41 CTATCGA 15 0.008442628 49.96052 30 GAATACG 15 0.008442628 49.96052 30 CCTATCG 15 0.008454094 49.94338 29 ATAGCTC 20 4.2667202E-4 49.77257 22 TGACGAT 15 0.00859259 49.738552 20 >>END_MODULE