FastQCFastQC Report
Fri 17 Jun 2016
SRR1526550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences472206
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC29220.6187977281101892No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT23000.48707555600733576No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT21410.45340381104856775No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC20460.4332854728656561No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC20200.4277794013629645No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC11530.24417309394628617No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT9770.20690122531268132No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8110.17174707648780405No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC7830.16581746102336692No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC6790.14379317501260044No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT6750.1429460870891094No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT6200.1312986281411079No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC6040.1279102764471438No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC5750.12176888900183394No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT5350.11329800976692375No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA5320.11266269382430551No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.11202737788168723No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC5040.10673307835986835No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5030.10652130637899561No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG4980.10546244647463184No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG4770.10101523487630398No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGACG201.9748908E-458.20210656
ACCGTAA150.004638994658.14635543
CGGGTAT201.9978816E-458.06600636
ATGCGAA150.00466446258.06600636
CGGTCAA150.00468018658.01667432
CCGCAAT202.0062945E-458.01667432
TCGATAA150.004735531857.8446610
ATAGTCG150.004735531857.844669
CGAGGGG291000.056.632114
GCGAGGG473850.056.42253
GGCGAGG479250.056.3962822
GGGCGAG482000.056.338541
CGAGGGT23600.056.0063824
CGAGGGA125750.055.8436744
GAGGGGT32700.055.721925
GAGGGGG145850.055.603855
AGGGATC9850.055.4956446
GAGGGAT34350.054.56095
GAGGGGA66550.054.454825
AGGGGGG87750.054.350916
Produced by FastQC (version 0.11.5)