FastQCFastQC Report
Fri 17 Jun 2016
SRR1526549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459633
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC23460.5104072161920489No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT23450.5101896513087616No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT17360.37769263738678466No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC17090.3718183855380271No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC16720.3637684848563963No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9380.20407586052350463No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT8140.17709781499587715No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6290.13684831158772326No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC5670.12335928882390951No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT5570.12118363999103633No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT5450.1185728613915885No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC4670.10160280049517768No Hit
GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG4640.10095010584531572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTCA150.00459288658.29295338
CGTCGAT201.9885844E-458.1204540
TAAGTAG150.00469975257.95530325
GACGTAA202.044145E-457.79738217
CACATAT150.004756951757.77849220
CGCATAC150.004756951757.77849220
CGAGGGG279350.056.4798974
GCGAGGG459050.056.2942353
GGGCGAG467800.056.2722321
GGCGAGG464500.056.2552722
CGAGGGA120550.055.729044
GAGGGAT30550.055.56725
CGAGGGT23200.055.3763584
GAGGGGG133650.055.3429765
GAGGGGA66200.055.0804825
AGGGATC9750.054.7796826
GAGGGGT32950.054.4988565
CGAGGGC37900.054.3890384
AGGGGGG81450.054.054416
GAGGGGC54400.054.025815
Produced by FastQC (version 0.11.5)