##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526549_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 459633 Sequences flagged as poor quality 0 Sequence length 64 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.05450653020997 39.0 38.0 40.0 35.0 40.0 2 37.69841808573362 39.0 38.0 40.0 33.0 40.0 3 37.76551074444176 39.0 38.0 40.0 33.0 40.0 4 37.39582231911112 39.0 38.0 40.0 33.0 40.0 5 37.804863880530775 39.0 38.0 40.0 35.0 40.0 6 36.72058359604293 39.0 36.0 40.0 31.0 40.0 7 38.22796013341079 39.0 38.0 40.0 36.0 40.0 8 38.08120391703816 39.0 38.0 40.0 35.0 40.0 9 38.28482506695559 39.0 38.0 40.0 35.0 40.0 10 37.0266516982027 39.0 36.0 40.0 31.0 40.0 11 37.00476902224166 39.0 36.0 40.0 32.0 40.0 12 37.08506569371651 39.0 36.0 40.0 32.0 40.0 13 37.14037068704815 39.0 36.0 40.0 32.0 40.0 14 37.0550047537927 39.0 36.0 40.0 32.0 40.0 15 36.964084389066926 39.0 36.0 40.0 32.0 40.0 16 36.807951126224616 39.0 36.0 40.0 32.0 40.0 17 36.39831778832242 38.0 36.0 40.0 31.0 40.0 18 36.62301009718623 38.0 36.0 40.0 31.0 40.0 19 36.563188456877555 38.0 36.0 40.0 31.0 40.0 20 36.40611313809061 38.0 35.0 40.0 31.0 40.0 21 36.380366509802386 38.0 36.0 40.0 31.0 40.0 22 36.303898980273395 38.0 35.0 40.0 30.0 40.0 23 36.16391773436633 38.0 35.0 40.0 30.0 40.0 24 36.022174212904645 38.0 35.0 40.0 30.0 40.0 25 35.96514393004854 38.0 35.0 40.0 30.0 40.0 26 36.121449069148646 39.0 36.0 40.0 30.0 40.0 27 35.56726344714152 38.0 35.0 40.0 28.0 40.0 28 35.46040863036379 38.0 35.0 40.0 28.0 40.0 29 35.34512970130517 38.0 35.0 40.0 28.0 40.0 30 35.20079063078587 38.0 35.0 40.0 27.0 40.0 31 34.72115361603714 38.0 34.0 40.0 26.0 40.0 32 34.96338165449391 38.0 34.0 40.0 27.0 40.0 33 34.835081467170546 38.0 34.0 39.0 26.0 40.0 34 34.13723122578231 38.0 33.0 39.0 23.0 40.0 35 34.18983623891235 38.0 33.0 39.0 24.0 40.0 36 33.410259924766066 38.0 33.0 39.0 20.0 40.0 37 33.17326649740119 38.0 32.0 39.0 19.0 40.0 38 33.11565966760437 37.0 32.0 39.0 19.0 40.0 39 32.61275408858807 37.0 31.0 39.0 15.0 40.0 40 32.74062784874019 37.0 32.0 39.0 17.0 40.0 41 32.21765843618713 37.0 31.0 39.0 8.0 40.0 42 31.87804182902446 36.0 31.0 39.0 4.0 40.0 43 31.83577114785057 36.0 30.0 39.0 4.0 40.0 44 31.699847487016815 36.0 30.0 39.0 4.0 40.0 45 31.442831563443008 36.0 30.0 39.0 4.0 40.0 46 31.062689145470408 36.0 30.0 39.0 4.0 40.0 47 30.80527290251135 36.0 29.0 39.0 4.0 40.0 48 30.560070752100046 35.0 29.0 39.0 4.0 40.0 49 30.407570822808633 35.0 29.0 39.0 4.0 40.0 50 30.020751338567944 35.0 28.0 39.0 4.0 40.0 51 29.489318652055008 35.0 27.0 38.0 4.0 40.0 52 29.368385211679755 35.0 27.0 38.0 4.0 40.0 53 29.084201961129857 35.0 26.0 38.0 4.0 40.0 54 28.873083525334344 34.0 26.0 38.0 4.0 40.0 55 28.67062199624483 34.0 25.0 38.0 4.0 40.0 56 28.100262600814126 34.0 23.0 38.0 4.0 40.0 57 27.679100499746536 34.0 22.0 38.0 4.0 39.0 58 27.375734553437198 33.0 21.0 38.0 4.0 39.0 59 26.999926027939683 33.0 19.0 37.0 4.0 39.0 60 26.825954620316644 33.0 17.0 37.0 4.0 39.0 61 26.425702680181796 33.0 4.0 37.0 4.0 39.0 62 26.02505477195937 33.0 4.0 37.0 4.0 39.0 63 25.810838212225843 33.0 4.0 36.0 4.0 39.0 64 25.71227914444785 33.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.1722596437372914 2 2 0.016649876674804887 2 3 -0.20156167538592484 2 4 -0.047498967298558625 2 5 -0.06181227433843617 2 6 -0.0692875336192813 2 7 0.033993887277731005 2 8 0.3206166204627152 2 9 -0.045452733042964155 2 10 -0.10157530103995072 2 11 -0.3393663536413598 2 12 -0.29018318277282873 2 13 -0.02058509599361713 2 14 -0.057918331780939525 2 15 0.28745431584490433 2 16 0.14601956457688203 2 17 0.37766454758804713 2 18 -0.11774575856550484 2 19 -0.27069944781914757 2 20 -0.00473825553279994 2 21 -0.40542577073898656 2 22 -1.159879806549469 2 23 -0.6617024303835066 2 24 -0.7483638526380076 2 25 -0.5590104354383953 2 26 -0.3897200768112512 2 27 -0.07306612141687907 2 28 0.10629650776296273 2 29 0.012094549733639326 2 30 -0.6305397508113444 2 31 0.10151092187296484 2 32 0.046152710377306505 2 33 -0.24397902759739765 2 34 -0.13102533763203894 2 35 -0.27952195810889435 2 36 -0.9766303225030128 2 37 -1.1349616601990178 2 38 -1.60263378894712 2 39 -4.708453912681637 2 40 -1.696323148862131 2 41 -1.8532135515827122 2 42 -1.915634434318772 2 43 -1.8895446703525138 2 44 -1.9030531451056767 2 45 -2.680133921210661 2 46 -1.4839514963191114 2 47 -1.5703283183508745 2 48 -1.4095312309779509 2 49 -1.593470853399424 2 50 -1.2184249735446038 2 51 -1.0572674445972794 2 52 -1.6297551067256926 2 53 -1.131626993588597 2 54 -1.7935123691408243 2 55 -1.4624339862733287 2 56 -0.8467813371169512 2 57 -1.5467429675777886 2 58 -1.197709273344497 2 59 -1.088592635582799 2 60 -0.9880418401818503 2 61 -0.9565642927532068 2 62 -0.992997970861456 2 63 -1.0266213297115563 2 64 -0.7978783379799097 3 1 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119 55 -0.47123521939290214 119 56 -0.8807026468330292 119 57 -0.6954696032761767 119 58 -0.8635359737501034 119 59 -0.22481447084770423 119 60 -0.3627386895192579 119 61 -0.12368250704455619 119 62 -0.19186480552137652 119 63 0.020462374066188005 119 64 -0.24999236997578578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 420.0 5 70.0 6 164.0 7 237.0 8 472.0 9 442.0 10 760.0 11 950.0 12 1219.0 13 1762.0 14 1957.0 15 2430.0 16 2992.0 17 3296.0 18 3566.0 19 4173.0 20 4620.0 21 5931.0 22 7284.0 23 6892.0 24 7541.0 25 7692.0 26 7445.0 27 8509.0 28 9832.0 29 11218.0 30 13168.0 31 15239.0 32 18090.0 33 22872.0 34 28461.0 35 36727.0 36 48091.0 37 60466.0 38 65572.0 39 49030.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.29246976352563 0.5460949857166789 0.07810681269812261 0.08332843805955699 8 99.222386220649 0.45215672444345756 0.20267593185212276 0.12278112305542131 9 98.65479632663451 0.8356667167065898 0.2134311505048593 0.2961058061540403 10 60.64447069727369 26.13824507813843 5.140840627196046 8.076443597391831 11 41.33384678645789 23.68933475185637 16.799272463030288 18.177545998655447 12 39.20236362489204 15.071372159962404 22.55669196946259 23.169572245682968 13 29.961077642379898 15.502585758637869 26.141726116271023 28.394610482711208 14 23.99610123729149 18.256086921522172 33.237169654920336 24.510642186266 15 28.765408173708174 18.90591895970195 29.777050993678866 22.55162187291101 16 36.15500785571596 18.82825794602405 23.08470681423517 21.932027384024824 17 35.212553209102516 19.268992980973255 22.740600995995976 22.77785281392826 18 33.52348504132645 20.885358536049413 22.720953456344517 22.870202966279617 19 32.907993986506625 23.28161816057594 20.87491542165162 22.935472431265815 20 30.552509030769897 26.340688514601556 21.466031248639943 21.640771205988596 21 29.75439100325695 22.16420492001227 26.713051499783525 21.36835257694726 22 27.50672819401566 19.881514164561725 29.36451473240607 23.247242909016542 23 29.17109955116365 20.442396433676432 27.774333000459063 22.61217101470086 24 31.585108211545034 22.303088059633296 22.595728108022254 23.516075620799416 25 31.874659875051698 20.39269466031041 21.255142689218314 26.477502775419577 26 25.500125126486555 19.4844788753849 25.227022968870706 29.788373029257837 27 23.149710309831157 18.739400914627502 27.34051782390151 30.770370951639837 28 23.872725291489594 22.917075296938 23.855290399912825 29.354909011659586 29 28.53383581488057 24.215616865081525 22.747383211162063 24.503164108875843 30 29.72499302455357 19.930812290736608 26.73492431640625 23.609270368303573 31 29.40813487477596 19.248263260491303 28.587626303372303 22.755975561360437 32 34.97979872821335 17.541764549492026 25.85152610039182 21.6269106219028 33 33.958787614478844 18.67684256432621 26.407762756214563 20.956607064980375 34 27.06367924528302 20.51930468204053 33.24576345213138 19.171252620545072 35 24.650208184746784 27.59598418275764 29.541295609253915 18.21251202324166 36 26.657003270128314 27.188344546657177 26.55920504010164 19.595447143112864 37 28.113400351100903 26.891534276007235 24.43382796055792 20.561237412333945 38 29.890821447985733 24.792111025483727 24.80848453358425 20.50858299294629 39 29.34375280618335 21.96609144927187 24.318357531303317 24.371798213241462 40 28.87793607672889 21.232125854945362 24.641643591513002 25.248294476812745 41 23.649277502741864 22.889639472338864 25.590429041462208 27.87065398345706 42 22.222295116382817 21.073774722054566 28.586174018771704 28.117756142790913 43 22.140167336566932 19.213745566193293 30.39699791376 28.249089183479782 44 25.88862002408854 18.039079389436456 27.42595835000077 28.646342236474243 45 25.445329344721866 19.72088466702529 26.70051738238626 28.133268605866586 46 24.60833867358968 22.07082382840608 26.36001565989165 26.960821838112594 47 22.797102463605825 21.96707901735722 29.40010848264278 25.835710036394175 48 24.24660589943665 22.593075335036218 29.688757479267995 23.471561286259142 49 28.93253450052169 19.79386088672192 27.54201418737573 23.73159042538066 50 24.35085552621322 19.938994214742113 27.646394177078815 28.063756081965852 51 21.937091889431578 22.64696356984052 29.907611645046657 25.508332895681253 52 22.384871768498314 22.849258229072518 29.11453625912852 25.651333743300647 53 24.37922904658601 19.540247498418385 31.5171920115759 24.56333144341971 54 22.777883569394557 19.440194539839297 30.286972528470713 27.49494936229543 55 24.897098618932105 19.969436100431746 27.384684771471356 27.74878050916479 56 22.96583558512199 21.396663703210606 24.959161239746518 30.67833947192089 57 24.454385607648128 25.565868964958494 24.27986783070243 25.69987759669095 58 25.068627902034628 28.3827037969359 22.96608368551004 23.582584615519437 59 27.534336310717052 27.87983550995638 23.494602807372694 21.09122537195388 60 30.870134111247243 23.641189401726635 23.15287454823205 22.335801938794074 61 29.20184997021828 21.954819382642516 23.726174625976665 25.11715602116254 62 25.364393174486015 21.18003158256695 25.2956211493689 28.159954093578136 63 23.35158577185912 21.267960399509374 24.968021727702823 30.412432100928683 64 24.056082633323395 21.158786805324166 25.471739473545764 29.31339108780667 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 3.5 8 7.0 9 5.5 10 12.5 11 21.0 12 61.0 13 195.0 14 289.0 15 343.5 16 404.5 17 411.0 18 695.5 19 980.0 20 1368.0 21 1736.0 22 1716.0 23 1856.5 24 2304.0 25 2611.0 26 3196.5 27 4241.0 28 4700.0 29 4953.0 30 5274.0 31 5342.0 32 4896.5 33 4451.0 34 4951.0 35 5302.0 36 5153.0 37 4991.5 38 4906.0 39 4982.0 40 5169.5 41 5571.5 42 5786.0 43 6247.0 44 6708.0 45 7234.0 46 8020.0 47 8280.0 48 8880.5 49 11029.0 50 12577.0 51 12570.0 52 12795.0 53 13027.0 54 13299.0 55 14837.0 56 16103.0 57 17933.5 58 19764.0 59 22396.5 60 26054.5 61 27080.0 62 31384.5 63 38203.5 64 40718.0 65 39711.0 66 33679.5 67 28655.0 68 25347.5 69 22040.0 70 19687.5 71 14669.0 72 12003.0 73 10467.5 74 7632.0 75 6332.0 76 5503.5 77 3813.0 78 2951.0 79 2369.5 80 1428.0 81 1068.0 82 851.0 83 634.0 84 503.0 85 290.5 86 209.0 87 179.0 88 103.5 89 58.0 90 41.0 91 17.5 92 11.0 93 9.5 94 8.0 95 4.5 96 1.0 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.00130538929972391 8 0.06070060243716182 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08267465564918097 16 0.020668663912295243 17 0.12945110555595443 18 0.0 19 0.0 20 0.020233534145720607 21 0.0 22 0.0 23 0.0 24 0.00522155719889564 25 0.05286826663881836 26 0.021321358562157198 27 0.18862875381010502 28 0.17035330361397027 29 0.12575250254007003 30 0.1916746621761275 31 0.22082835653662813 32 0.1633912273487761 33 0.22474452443579984 34 0.3726886450711763 35 0.24932935624726685 36 0.3365728744454815 37 0.35789423300763873 38 0.34288225606081374 39 0.6640080237928957 40 0.3718183855380271 41 0.4166367514952147 42 0.5110599108419108 43 0.5114950406084855 44 0.4686347586008837 45 0.5071437429427391 46 0.5252016282555865 47 0.5258543229054484 48 0.5154112085076572 49 0.536515002186527 50 0.6429042301140258 51 0.5763293758281063 52 0.5843792765097371 53 0.6139681006368124 54 0.6004790778729987 55 0.6276746882839135 56 0.6437744896471751 57 0.6409461461644399 58 0.6931617181533963 59 0.6944671074531202 60 0.7331936566782629 61 0.6476906575463468 62 0.6640080237928957 63 0.6685768863419294 64 0.7503812824579611 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 459633.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.9662410776247 #Duplication Level Percentage of deduplicated Percentage of total 1 87.51255297255558 50.72773742925559 2 4.729872527058217 5.483458623397812 3 2.162243125330877 3.76011318814099 4 1.298163657662258 3.009986701530461 5 0.9116835229659892 2.6423433439372364 6 0.6230788426369476 2.1670523041596415 7 0.4834214377182247 1.9615486520606584 8 0.3609760165134296 1.6739538237166478 9 0.28802251627536274 1.5026024352781586 >10 1.4804229770958581 15.31052309948624 >50 0.09892580564511815 3.9512636135017036 >100 0.04694783970797063 5.115629658865523 >500 0.0020120502731987414 0.7872591905766375 >1k 0.001676708560998951 1.9065279360927556 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 2346 0.5104072161920489 No Hit GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 2345 0.5101896513087616 No Hit GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT 1736 0.37769263738678466 No Hit GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 1709 0.3718183855380271 No Hit GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 1672 0.3637684848563963 No Hit GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 938 0.20407586052350463 No Hit GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT 814 0.17709781499587715 No Hit GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 629 0.13684831158772326 No Hit GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC 567 0.12335928882390951 No Hit GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT 557 0.12118363999103633 No Hit GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT 545 0.1185728613915885 No Hit GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC 467 0.10160280049517768 No Hit GGGCGAGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG 464 0.10095010584531572 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0073972060317688245 0.0 0.0 0.0 0.0 7 0.0073972060317688245 0.0 0.0 0.0 0.0 8 0.007832335798343462 0.0 0.0 0.0 0.0 9 0.009137725098067371 0.0 0.0 0.0 0.0 10 0.01196606858080251 0.0 0.0 0.0 0.0 11 0.019580839495858652 0.0 0.0 0.0 0.0 12 0.02088622879558256 0.0 0.0 0.0 0.0 13 0.022191618095306474 0.0 0.0 0.0 0.0 14 0.025672656227903566 0.0 0.0 0.0 0.0 15 0.02763074017748943 0.0 0.0 0.0 0.0 16 0.028936129477213343 0.0 0.0 0.0 0.0 17 0.030894213426799207 0.0 0.0 0.0 0.0 18 0.03328742714295971 0.0 0.0 0.0 0.0 19 0.03546307597583289 0.0 0.0 0.0 0.0 20 0.0402495034081539 0.0 0.0 0.0 0.0 21 0.041337327824590486 0.0 0.0 0.0 0.0 22 0.04416567130732563 0.0 0.0 0.0 0.0 23 0.047429144556635404 0.0 0.0 0.0 0.0 24 0.050257488039370544 0.0 0.0 0.0 0.0 25 0.052215571988956405 0.0 0.0 0.0 0.0 26 0.05439122082182959 0.0 0.0 0.0 0.0 27 0.057654694071139365 0.0 0.0 0.0 0.0 28 0.06483433521962087 0.0 0.0 0.0 0.0 29 0.06722754893578137 0.0 0.0 0.0 0.0 30 0.06962076265194188 0.0 0.0 0.0 0.0 31 0.0711437168349531 0.0 0.0 0.0 0.0 32 0.07375449543440092 0.0 0.0 0.0 0.0 33 0.07593014426727411 0.0 0.0 0.0 0.0 34 0.07897605263329656 0.0 0.0 0.0 0.0 35 0.08550299913191611 0.0 0.0 0.0 0.0 36 0.08854890749793858 0.0 0.0 0.0 0.0 37 0.0911596860973864 0.0 0.0 0.0 0.0 38 0.09377046469683421 0.0 0.0 0.0 0.0 39 0.09768663259600595 0.0 0.0 0.0 0.0 40 0.10225549514503963 0.0 0.0 0.0 0.0 41 0.10747705234393527 0.0 0.0 0.0 0.0 42 0.11052296070995772 0.0 0.0 0.0 0.0 43 0.11269860954283091 0.0 0.0 0.0 0.0 44 0.11422156372584213 0.0 0.0 0.0 0.0 45 0.11835529650830119 0.0 0.0 0.0 0.0 46 0.12140120487432364 0.0 0.0 0.0 0.0 47 0.12379441859048415 0.0 0.0 0.0 0.0 48 0.12966867043924174 6.52694649861955E-4 0.0 0.0 0.0 49 0.13358483833841348 6.52694649861955E-4 0.0 0.0 0.0 50 0.13641318182114862 6.52694649861955E-4 0.0 0.0 0.0 51 0.1422874336699062 6.52694649861955E-4 0.0 0.0 0.0 52 0.1468562962189399 6.52694649861955E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTTCA 15 0.004592886 58.292953 38 CGTCGAT 20 1.9885844E-4 58.12045 40 TAAGTAG 15 0.004699752 57.955303 25 GACGTAA 20 2.044145E-4 57.797382 17 CACATAT 15 0.0047569517 57.778492 20 CGCATAC 15 0.0047569517 57.778492 20 CGAGGGG 27935 0.0 56.479897 4 GCGAGGG 45905 0.0 56.294235 3 GGGCGAG 46780 0.0 56.272232 1 GGCGAGG 46450 0.0 56.255272 2 CGAGGGA 12055 0.0 55.72904 4 GAGGGAT 3055 0.0 55.5672 5 CGAGGGT 2320 0.0 55.376358 4 GAGGGGG 13365 0.0 55.342976 5 GAGGGGA 6620 0.0 55.080482 5 AGGGATC 975 0.0 54.779682 6 GAGGGGT 3295 0.0 54.498856 5 CGAGGGC 3790 0.0 54.389038 4 AGGGGGG 8145 0.0 54.05441 6 GAGGGGC 5440 0.0 54.02581 5 >>END_MODULE