FastQCFastQC Report
Fri 17 Jun 2016
SRR1526548_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526548_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419172
Sequences flagged as poor quality0
Sequence length56
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC36470.8700485719465995No Hit
GGGCGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC29120.6947028904602407No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC26400.6298130600326358No Hit
GGGCGAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG21870.5217428645043085No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC13620.32492628324410977No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC10550.25168665846001165No Hit
GGGCGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT9940.2371341597244091No Hit
GGGCGAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT8470.20206502342713734No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC7260.17319859150897485No Hit
GGGCGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC5840.1393222829769164No Hit
GGGCGAGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCA5770.13765232410561773No Hit
GGGCGAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.13335814415084976No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT5400.12882539864303913No Hit
GGGCGAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC5140.12262269426392984No Hit
GGGCGAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.11665855543786322No Hit
GGGCGAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC4570.10902445774049793No Hit
GGGCGAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG4500.10735449886919927No Hit
GGGCGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC4250.10139036004313265No Hit
GGGCGAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA4210.10043609783096198No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATATA150.0080180550.6335535
ACGATAT150.00803346350.60895534
CACCGAT150.00803346350.60895534
ATTACGT150.00812639750.4619333
TACTAAT150.00812639750.4619333
CATTACG150.008145858550.43140432
TTACGTC150.008145858550.43140432
AGGTTAT204.0843984E-450.23692347
CGAACCG150.008275175550.2308738
ACCGAAT150.0083582650.10408441
CATACCG150.0083582650.10408441
ATTATGC150.00847802549.92407228
TACGACT150.00851822949.86435325
TTAGTAG150.008578801549.77504323
CGTCGAC150.00858690249.7631621
CTTAGTA150.00858690249.7631622
ACGTGTC150.00861123849.72754310
ATCGCGT150.00861123849.72754310
CGAGGGG257300.048.7729454
GGCGAGG423250.048.722572
Produced by FastQC (version 0.11.5)