FastQCFastQC Report
Fri 17 Jun 2016
SRR1526547_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526547_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11045
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2902.625622453598914No Hit
ACACCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG780.7062019013128112No Hit
ACACCCGGGGAGCTGCCCAGGTGTTCCTAGGAGCTGGGCTCTGGTTTTGGAGAACAGATCATGC680.615663196016297No Hit
ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.5975554549569941No Hit
ACACCCGGGCACACACTCACCTGTGTTGTACAATGACACAGTTTGGCCACACGCCCATTGGGAG490.4436396559529199No Hit
ACACCCGGGTGTAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.4164780443639656No Hit
ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT360.3259393390674513No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC320.2897238569488456Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.26256224535989137No Hit
ACACCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGC270.24445450430058852No Hit
ACACCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA250.22634676324128564Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.2082390221819828No Hit
ACACCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG220.1991851516523314No Hit
ACACCCGGGTGTAGCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1810774105930285No Hit
ACACCCGAGCTGTACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1720235400633771No Hit
ACACCCGGGAAGTACGCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.16296966953372566No Hit
ACACCCGGGTGTAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.16296966953372566No Hit
ACACCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG180.16296966953372566No Hit
ACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.16296966953372566No Hit
ACACCCGGGTGTAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15391579900407426No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTG170.15391579900407426No Hit
ACACCCGGGGAACTATAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACA170.15391579900407426No Hit
ACACCCGGGGGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGGGTCCTCCGGGGCGAAATTCC160.1448619284744228No Hit
ACACCCGGGCAATTAAGTGGTAGGGGAGCATTGTGTAAGCCTGTGAAGGTGTGTTGTAAAGCAT160.1448619284744228No Hit
ACACCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1358080579447714No Hit
ACACCCGGGTGTAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1358080579447714No Hit
ACACCCGGGGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAA140.12675418741511996No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.12675418741511996No Hit
ACACCCGGGGAGGTGAGGAAAGACAGACACATATAGCAAGCTCTACTACAACTTTACAGTGTGA140.12675418741511996No Hit
ACACCCGGGATGGTATCAACGAGAGCCGACTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12675418741511996No Hit
ACACCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG140.12675418741511996No Hit
ACACCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12675418741511996No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGATCGGAAGAGCTCGTATGCCGTCTTCTGCTT140.12675418741511996Illumina Single End Adapter 2 (96% over 33bp)
ACACCCGGGAAGCTGGACAACGCAGAGTCGACTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTT140.12675418741511996No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAA130.11770031688546854Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGATGGTACAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11770031688546854No Hit
ACACCCGGGGAGTGGTACAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.11770031688546854No Hit
ACACCCGGGGTCGACTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1086464463558171No Hit
ACACCCGGGGAGTCGACTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1086464463558171No Hit
ACACCCGGGCTACACCCGGGCAGATACGATTATGAAAATCATTTATGAGAAAGGATGTTTGCTT120.1086464463558171No Hit
ACACCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG120.1086464463558171No Hit
ACACCCGGGACATGTTCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1086464463558171No Hit
ACACCCGGGTGACACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1086464463558171No Hit
ACACCCGGGAACTGTATCACAGATCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1086464463558171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACATC201.8966213E-458.16438348
ACATTTT150.004513602258.16438341
CTGAAAA201.8966213E-458.16438343
AAAACAT201.8966213E-458.16438347
GCCCCCG150.004513602258.16438358
CGGGGGT258.1983035E-657.96
CGGGGGA258.1983035E-657.96
CGGGGAA258.1983035E-657.96
CCGGGCT258.1983035E-657.95
GGAGAGT150.00459632357.8999988
ACTGCCC150.00459632357.89999816
GTGCACT150.00459632357.89999811
CGGGGCT201.9402217E-457.8999986
CGGGGCA150.00459632357.8999986
ACACCCA303.4699406E-757.8999981
ACCCGGC150.00459632357.8999983
CACTGCC150.00459632357.89999815
CGGGCTA150.00459632357.8999986
GGGAGAG201.9402217E-457.8999987
CGGGTGG303.4699406E-757.8999986
Produced by FastQC (version 0.11.5)