FastQCFastQC Report
Fri 17 Jun 2016
SRR1526546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10823
Sequences flagged as poor quality0
Sequence length64
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2462.272937263235702No Hit
ACACCCGGGGAGCTGCCCAGGTGTTCCTAGGAGCTGGGCTCTGGTTTTGGAGAACAGATCATGC810.7484061720410238No Hit
ACACCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG660.6098124364778712No Hit
ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.5266561951399797No Hit
ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.452739536172965No Hit
ACACCCGGGTGTAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.4342603714312113No Hit
ACACCCGGGCACACACTCACCTGTGTTGTACAATGACACAGTTTGGCCACACGCCCATTGGGAG330.3049062182389356No Hit
ACACCCGGGTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.24022914164279777No Hit
ACACCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG250.23098955927192089No Hit
ACACCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.2125103945301672No Hit
ACACCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGC230.2125103945301672No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC220.2032708121592904Illumina Single End Adapter 2 (96% over 31bp)
ACACCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.2032708121592904No Hit
ACACCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAA210.19403122978841356Illumina Single End Adapter 2 (97% over 34bp)
ACACCCGGGGAGTGGTACAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1755520650466599No Hit
ACACCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.15707290030490623No Hit
ACACCCGGGAAGCTGGACAACGCAGAGTCGACTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTT160.14783331793402937No Hit
ACACCCGGGTGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.13859373556315255No Hit
ACACCCGGGAAGTACGCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.13859373556315255No Hit
ACACCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG150.13859373556315255No Hit
ACACCCAGGAACATGTTCAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.12935415319227572No Hit
ACACCCGGGGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGTAAACATAA130.12011457082139888No Hit
ACACCCGGGTGTAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.12011457082139888No Hit
ACACCCGAGCTGTACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.12011457082139888No Hit
ACACCCGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTGTCAGGAACCCTGAG120.11087498845052204No Hit
ACACCCGGGTGTAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11087498845052204No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTG120.11087498845052204No Hit
ACACCCGGGGAACTATAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGAATGCTTTACACA120.11087498845052204No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1016354060796452No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTTT110.1016354060796452No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTC110.1016354060796452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACT150.004492409558.224312
GTAGTAC150.004492409558.224311
ATGATCA150.004576693357.95348728
CCAGAGG150.004576693357.95348754
CCATGAT150.004576693357.95348726
GCCATGA150.004576693357.95348725
ACCCGGA452.7284841E-1157.685193
CGGGCCA258.370507E-657.685196
CGGGAGT258.370507E-657.685196
CGGGGGG700.057.6851846
GTGTAGT150.00466214957.6851849
CCCGGAT150.00466214957.6851844
CCCGGAG150.00466214957.6851844
CCCGGAA150.00466214957.6851844
ACACCCC150.00466214957.6851841
ACACCCA150.00466214957.6851841
CGGGCTT150.00466214957.6851846
CGGGTGA150.00466214957.6851846
CGGGCAC150.00466214957.6851846
CGGGTAT150.00466214957.6851846
Produced by FastQC (version 0.11.5)