FastQCFastQC Report
Fri 17 Jun 2016
SRR1526545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9616
Sequences flagged as poor quality0
Sequence length56
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3513.6501663893510816No Hit
ACACCCGGGGAGCTGCCCAGGTGTTCCTAGGAGCTGGGCTCTGGTTTTGGAGAACA860.894342762063228No Hit
ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA750.7799500831946755No Hit
ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.6551580698835274No Hit
ACACCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA530.5511647254575708No Hit
ACACCCGGGTGTAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.509567387687188No Hit
ACACCCGGGCACACACTCACCTGTGTTGTACAATGACACAGTTTGGCCACACGCCC280.2911813643926789No Hit
ACACCCGGGTGTAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.2911813643926789No Hit
ACACCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA250.25998336106489184No Hit
ACACCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG240.24958402662229617No Hit
ACACCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.21838602329450912No Hit
ACACCCGGGTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.20798668885191346No Hit
ACACCCGGGTGTAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.20798668885191346No Hit
ACACCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG180.18718801996672213Illumina Single End Adapter 2 (96% over 30bp)
ACACCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.18718801996672213No Hit
ACACCCGGGCAATTAAGTGGTAGGGGAGCATTGTGTAAGCCTGTGAAGGTGTGTTG170.17678868552412644No Hit
ACACCCGGGTGTAGCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.16638935108153077No Hit
ACACCCGGGTGTAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.16638935108153077No Hit
ACACCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.1559900166389351No Hit
ACACCCGGGTACTAAATAGTCATTCTTTTCTTGGTATTTCTTCCTTGGAAAAATCA140.14559068219633944No Hit
ACACCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG130.13519134775374378No Hit
ACACCCGGGGAGTGGTACAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT130.13519134775374378No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG130.13519134775374378Illumina Single End Adapter 2 (95% over 23bp)
ACACCCGGGAAGTACGCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.13519134775374378No Hit
ACACCCAGGAACATGTTCAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT120.12479201331114809No Hit
ACACCCGGGAAGCTGGACAACGCAGAGTCGACTTTTTTTTTCTTTTTTTTTTTTTT120.12479201331114809No Hit
ACACCCGGGGGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGGGTCCTCCGGGGC120.12479201331114809No Hit
ACACCCGGGATGGTATCAACGAGAGCCGACTTTTGTTTTTTTTTTTTTTTTTTTTT120.12479201331114809No Hit
ACACCCGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTGTCAGGA110.11439267886855242No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT110.11439267886855242No Hit
ACACCCGGGTGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11439267886855242No Hit
ACACCCGGGAGCCCAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.11439267886855242No Hit
ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11439267886855242No Hit
ACACCCGGGGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGT110.11439267886855242No Hit
ACACCCGGGTGGACGCAGAGTCGACTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTT100.10399334442595673No Hit
ACACCCGGGAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAATGTGTCTGC100.10399334442595673No Hit
ACACCCGAGCTGTACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10399334442595673No Hit
ACACCCGGGGAGTCGACTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.10399334442595673No Hit
ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGTGTAGCA100.10399334442595673No Hit
ACACCCGGGATGTTCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTT100.10399334442595673No Hit
ACACCCGGCAGCCGCGAAGCAGCAACCATGCGTGAGTGCATCTCCATCCATGTTGG100.10399334442595673No Hit
ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT100.10399334442595673No Hit
ACACCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC100.10399334442595673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG402.2773747E-949.8958366
CGGGGTA204.019596E-449.8958366
ACACCCC204.019596E-449.8958361
CGGGTGC204.019596E-449.8958366
CGGGAGA204.019596E-449.8958366
CCGGGTG1600.049.8958365
CCGGGTA354.6678906E-849.8958365
CCGGGGT700.049.8958365
ATCCCCA150.00824792649.89583224
GGAGAAA150.00824792649.89583220
GAGAAAG150.00824792649.89583221
TCAAGAT150.00824792649.89583219
AATTCGT150.00824792649.89583213
CGGGGGC150.00824792649.8958326
CGGGGCT251.959521E-549.8958326
CCCGGGT2200.049.8958324
ACCCGGC150.00824792649.8958323
TAGCATT150.00824792649.89583212
CGGGCGT150.00824792649.8958326
CGTGGAG150.00824792649.89583217
Produced by FastQC (version 0.11.5)