##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526545_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9616 Sequences flagged as poor quality 0 Sequence length 56 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.11844841930117 39.0 38.0 40.0 35.0 40.0 2 37.93240432612313 39.0 38.0 40.0 35.0 40.0 3 36.70403494176373 39.0 35.0 39.0 31.0 40.0 4 37.973689683860236 39.0 38.0 40.0 35.0 40.0 5 38.018094841930115 39.0 38.0 40.0 35.0 40.0 6 38.09172212978369 39.0 38.0 40.0 35.0 40.0 7 37.71505823627288 39.0 38.0 40.0 35.0 40.0 8 37.90006239600665 39.0 38.0 40.0 35.0 40.0 9 37.85472129783694 39.0 38.0 40.0 35.0 40.0 10 37.03202995008319 39.0 37.0 40.0 33.0 40.0 11 37.33267470881864 39.0 38.0 40.0 33.0 40.0 12 37.12271214642263 39.0 36.0 40.0 33.0 40.0 13 37.09369800332779 39.0 36.0 40.0 33.0 40.0 14 37.05917221297837 39.0 37.0 40.0 33.0 40.0 15 37.12624792013311 39.0 37.0 40.0 33.0 40.0 16 37.04960482529118 39.0 37.0 40.0 33.0 40.0 17 37.1189683860233 39.0 37.0 40.0 33.0 40.0 18 36.98055324459234 39.0 37.0 40.0 33.0 40.0 19 36.65588602329451 39.0 36.0 40.0 31.0 40.0 20 36.811356073211314 39.0 36.0 40.0 32.0 40.0 21 36.634879367720465 39.0 36.0 40.0 32.0 40.0 22 36.455074875207984 38.0 36.0 40.0 31.0 40.0 23 36.27599833610649 38.0 36.0 40.0 31.0 40.0 24 36.27828618968386 38.0 36.0 40.0 31.0 40.0 25 36.15443011647255 38.0 36.0 40.0 31.0 40.0 26 35.8044925124792 38.0 35.0 39.0 30.0 40.0 27 35.73024126455907 38.0 35.0 39.0 29.0 40.0 28 35.22774542429285 38.0 35.0 39.0 28.0 40.0 29 35.31281198003328 38.0 35.0 39.0 28.0 40.0 30 34.99459234608985 38.0 35.0 39.0 27.0 40.0 31 34.574147254575706 38.0 35.0 39.0 26.0 40.0 32 34.04346921797005 38.0 33.0 39.0 23.0 40.0 33 34.26955074875208 38.0 34.0 39.0 24.0 40.0 34 34.357945091514146 38.0 34.0 39.0 25.0 40.0 35 33.89777454242928 38.0 33.0 39.0 22.0 40.0 36 33.39579866888519 38.0 33.0 39.0 14.0 40.0 37 32.830178868552416 38.0 33.0 39.0 4.0 40.0 38 32.97826539101497 38.0 33.0 39.0 7.0 40.0 39 32.91649334442596 38.0 33.0 39.0 4.0 40.0 40 32.42470881863561 38.0 32.0 39.0 4.0 40.0 41 32.23128119800333 38.0 32.0 39.0 4.0 40.0 42 32.02495840266223 37.0 32.0 39.0 4.0 40.0 43 31.971921797004992 37.0 32.0 39.0 4.0 40.0 44 31.3966306156406 37.0 31.0 39.0 4.0 40.0 45 31.46162645590682 37.0 31.0 39.0 4.0 40.0 46 30.87728785357737 36.0 30.0 39.0 4.0 40.0 47 30.621672212978368 36.0 30.0 39.0 4.0 40.0 48 30.27475041597338 36.0 29.0 39.0 4.0 40.0 49 30.146318635607322 36.0 29.0 39.0 4.0 40.0 50 29.744904326123127 36.0 28.0 39.0 4.0 40.0 51 29.026102329450914 35.0 26.0 39.0 4.0 40.0 52 28.814059900166388 35.0 25.0 39.0 4.0 40.0 53 28.418573211314477 35.0 22.0 39.0 4.0 40.0 54 28.00717554076539 35.0 15.0 39.0 4.0 40.0 55 27.39715058236273 35.0 4.0 39.0 4.0 40.0 56 16.37292013311148 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -3.101185064935059 1 2 -1.3307777176527225 1 3 -1.5131803150553083 1 4 -2.788105759980766 1 5 -2.41805555555554 1 6 -3.472772967772954 1 7 -5.653859728234725 1 8 -4.839669011544011 1 9 -4.754932659932663 1 10 -2.2160663179413262 1 11 -1.7841305916305856 1 12 -5.201494408369399 1 13 -2.136485088985097 1 14 -0.9510173160173139 1 15 -1.5393903318903313 1 16 -1.2945932539682516 1 17 -2.940891955266949 1 18 -2.5102095358345196 1 19 -0.27245220057719877 1 20 -1.955346320346294 1 21 -1.676181156806166 1 22 0.2794793169793266 1 23 0.17624639249638108 1 24 0.586565055315063 1 25 1.3040842953343201 1 26 0.35837572150072816 1 27 -0.110109427609423 1 28 0.7470193001443093 1 29 1.8891155603655534 1 30 0.3286961880711843 1 31 3.7835245310245327 1 32 3.2862493987493977 1 33 3.833097943722933 1 34 3.0190518278018175 1 35 3.338736171236171 1 36 3.7888726551226526 1 37 5.5412608225108215 1 38 4.912557720057727 1 39 4.810557058682065 1 40 5.72706950456951 1 41 6.042037337662354 1 42 5.4805038480038455 1 43 5.534770322270322 1 44 6.569978956228951 1 45 6.61820406445408 1 46 5.881433381433375 1 47 6.524251142376148 1 48 7.475527898027899 1 49 6.304518999518994 1 50 6.699805194805204 1 51 8.114340728715739 1 52 8.009850288600312 1 53 7.922207792207789 1 54 7.371746332371352 1 55 9.881816077441083 1 56 7.43462151274651 2 1 -0.6900739538239478 2 2 0.7470000601250533 2 3 -0.2354025372775297 2 4 0.40078312890811674 2 5 0.44861111111112706 2 6 -0.31721741221739563 2 7 0.457251382876386 2 8 0.37144209956710483 2 9 0.6450673400673352 2 10 1.2283781265031166 2 11 -2.4174639249639185 2 12 0.042950036075041 2 13 -0.40315175565176276 2 14 0.560093795093799 2 15 0.027276334776331623 2 16 -0.43903769841269735 2 17 -0.04089195526695022 2 18 0.06756824194325617 2 19 -0.09467442279942162 2 20 0.3890981240981475 2 21 0.04604106541605546 2 22 -1.0871873496873405 2 23 1.4540241702741596 2 24 0.1421206108706201 2 25 0.09297318422320444 2 26 -0.019402056277051827 2 27 0.9565572390572399 2 28 1.5914637445887507 2 29 1.8002266714766648 2 30 2.028696188071187 2 31 2.7835245310245327 2 32 0.48624939874940054 2 33 2.966431277056266 2 34 1.3190518278018146 2 35 1.9498472823472852 2 36 4.211094877344877 2 37 4.552371933621934 2 38 4.379224386724388 2 39 4.399445947570953 2 40 3.2604028379028485 2 41 2.586481782106798 2 42 3.7916149591149626 2 43 4.245881433381438 2 44 3.7366456228956153 2 45 4.084870731120741 2 46 1.6481000481000407 2 47 3.3686955868205892 2 48 2.2755278980278995 2 49 2.8267412217412122 2 50 2.3664718614718687 2 51 -0.16343704906203982 2 52 0.35429473304475323 2 53 0.42220779220778937 2 54 -1.0393647787397633 2 55 0.6040382996633049 2 56 -2.909822931697933 3 1 0.10083513708514857 3 2 0.17427278739778274 3 3 0.17368837181337682 3 4 -0.626489598364607 3 5 1.0667929292929443 3 6 0.4464189514189698 3 7 -0.08820316257816074 3 8 0.9078057359307365 3 9 0.8814309764309698 3 10 -0.22616732804233664 3 11 0.7007178932178988 3 12 -0.4843226911976828 3 13 0.8332118807118718 3 14 0.0691847041847069 3 15 0.21818542568542654 3 16 0.170053210678212 3 17 0.5136534992785045 3 18 0.6312046055796259 3 19 0.6871437590187597 3 20 0.307279942279969 3 21 0.45513197450696197 3 22 1.0673581048581156 3 23 0.6812968975468863 3 24 0.9603024290524402 3 25 1.4657004569504792 3 26 1.1260524891774963 3 27 1.6474663299663348 3 28 0.7369182900432989 3 29 2.3638630351130345 3 30 2.9650598244348174 3 31 2.238069985569986 3 32 2.558976671476671 3 33 1.2573403679653623 3 34 2.109960918710911 3 35 2.813483645983652 3 36 3.2838221500721474 3 37 4.288735569985569 3 38 3.6701334776334846 3 39 4.153991402116404 3 40 4.4240392015392125 3 41 5.75920905483407 3 42 4.682524050024053 3 43 3.1913359788359834 3 44 6.236645622895615 3 45 5.103052549302564 3 46 5.1844636844636725 3 47 3.605059223184224 3 48 4.293709716209719 3 49 3.526741221741215 3 50 3.457380952380962 3 51 2.5183811327561436 3 52 2.1815674603174813 3 53 -0.16870129870130057 3 54 0.987907948532964 3 55 1.2858564814814883 3 56 -1.8280047498797511 4 1 0.5099260461760551 4 2 1.322000060125056 4 3 0.8895974627224703 4 4 0.8507831289081196 4 5 1.1236111111111242 4 6 0.957782587782603 4 7 1.707251382876386 4 8 1.3964420995671034 4 9 1.4950673400673367 4 10 1.9783781265031166 4 11 2.2575360750360787 4 12 1.3679500360750438 4 13 1.8218482443482387 4 14 1.4100937950938004 4 15 0.37727633477633304 4 16 1.5109623015873055 4 17 1.3091080447330512 4 18 1.767568241943259 4 19 2.50532557720058 4 20 -3.135901875901851 4 21 -1.3289589345839445 4 22 0.1128126503126623 4 23 -0.6709758297258404 4 24 -2.10787938912938 4 25 -1.0570268157767941 4 26 2.8305979437229496 4 27 1.6815572390572413 4 28 2.441463744588752 4 29 1.7502266714766677 4 30 0.7036961880711843 4 31 1.9085245310245327 4 32 2.4112493987493977 4 33 2.916431277056269 4 34 4.0190518278018175 4 35 3.949847282347285 4 36 4.261094877344874 4 37 5.402371933621936 4 38 3.579224386724391 4 39 4.4494459475709505 4 40 6.060402837902846 4 41 6.236481782106797 4 42 6.34161495911496 4 43 6.770881433381437 4 44 4.861645622895615 4 45 5.534870731120744 4 46 6.173100048100039 4 47 5.093695586820591 4 48 6.475527898027899 4 49 6.276741221741215 4 50 7.241471861471869 4 51 4.33656295093796 4 52 3.7042947330447547 4 53 4.672207792207789 4 54 2.7606352212602374 4 55 1.729038299663305 4 56 1.6151770683020672 5 1 0.3849260461760551 5 2 -1.677999939874944 5 3 -1.6104025372775297 5 4 0.3507831289081196 5 5 0.4986111111111242 5 6 0.957782587782603 5 7 0.832251382876386 5 8 1.0214420995671034 5 9 1.1200673400673367 5 10 0.4783781265031166 5 11 1.6325360750360787 5 12 2.242950036075044 5 13 1.5718482443482387 5 14 2.0350937950938004 5 15 1.002276334776333 5 16 1.3859623015873055 5 17 1.0591080447330512 5 18 1.767568241943259 5 19 0.6303255772005798 5 20 1.989098124098149 5 21 1.2960410654160555 5 22 2.1128126503126623 5 23 1.9540241702741596 5 24 1.1421206108706201 5 25 2.817973184223206 5 26 2.5805979437229496 5 27 3.3065572390572413 5 28 3.066463744588752 5 29 2.6252266714766677 5 30 -0.6713038119288157 5 31 -1.8414754689754673 5 32 -0.5887506012506023 5 33 -2.208568722943731 5 34 -0.9809481721981825 5 35 -2.550152717652715 5 36 -2.613905122655126 5 37 -4.347628066378064 5 38 -0.9207756132756089 5 39 -1.8005540524290495 5 40 0.06040283790284562 5 41 -0.5135182178932034 5 42 0.9666149591149598 5 43 -0.22911856661856334 5 44 0.7366456228956153 5 45 -0.8401292688792559 5 46 3.0481000481000393 5 47 2.7186955868205906 5 48 2.4755278980278987 5 49 -0.7232587782587849 5 50 3.4914718614718687 5 51 3.08656295093796 5 52 1.2042947330447547 5 53 3.5472077922077894 5 54 2.8856352212602374 5 55 2.104038299663305 5 56 3.865177068302067 6 1 0.5099260461760551 6 2 1.197000060125056 6 3 1.1395974627224703 6 4 -0.14921687109188042 6 5 0.9986111111111242 6 6 -0.16721741221739705 6 7 0.332251382876386 6 8 0.1464420995671034 6 9 -1.5049326599326633 6 10 -0.02162187349688338 6 11 -0.24246392496392133 6 12 0.7429500360750438 6 13 -1.0531517556517613 6 14 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2.365177068302067 7 1 1.259926046176055 7 2 1.822000060125056 7 3 1.2645974627224703 7 4 1.4757831289081196 7 5 1.6236111111111242 7 6 1.457782587782603 7 7 1.832251382876386 7 8 1.5214420995671034 7 9 1.6200673400673367 7 10 2.4783781265031166 7 11 1.5075360750360787 7 12 2.367950036075044 7 13 2.3218482443482387 7 14 1.4100937950938004 7 15 1.752276334776333 7 16 1.5109623015873055 7 17 1.4341080447330512 7 18 2.142568241943259 7 19 2.50532557720058 7 20 1.739098124098149 7 21 1.6710410654160555 7 22 1.3628126503126623 7 23 1.7040241702741596 7 24 2.76712061087062 7 25 2.192973184223206 7 26 1.9555979437229496 7 27 2.8065572390572413 7 28 3.316463744588752 7 29 2.5002266714766677 7 30 3.5786961880711843 7 31 2.7835245310245327 7 32 2.9112493987493977 7 33 4.791431277056269 7 34 4.3940518278018175 7 35 5.324847282347285 7 36 6.261094877344874 7 37 6.027371933621936 7 38 6.079224386724391 7 39 6.8244459475709505 7 40 6.560402837902846 7 41 7.111481782106797 7 42 6.34161495911496 7 43 5.895881433381437 7 44 6.361645622895615 7 45 6.284870731120744 7 46 6.673100048100039 7 47 7.093695586820591 7 48 7.600527898027899 7 49 7.651741221741215 7 50 8.116471861471869 7 51 8.33656295093796 7 52 9.079294733044755 7 53 8.79720779220779 7 54 7.010635221260237 7 55 10.229038299663305 7 56 6.615177068302067 8 1 0.5099260461760551 8 2 1.5470000601250575 8 3 -0.03540253727752685 8 4 1.4007831289081167 8 5 1.5486111111111214 8 6 1.3827825877826 8 7 0.7572513828763832 8 8 1.2714420995671034 8 9 0.3450673400673381 8 10 1.7283781265031166 8 11 1.3825360750360787 8 12 0.9429500360750467 8 13 1.8968482443482415 8 14 1.7600937950938018 8 15 1.127276334776333 8 16 1.9609623015873083 8 17 1.9591080447330498 8 18 2.267568241943259 8 19 1.9053255772005784 8 20 1.989098124098149 8 21 2.146041065416057 8 22 1.9128126503126595 8 23 2.6540241702741625 8 24 2.7421206108706215 8 25 2.492973184223203 8 26 3.480597943722948 8 27 3.6565572390572427 8 28 2.0914637445887507 8 29 3.5002266714766677 8 30 3.028696188071187 8 31 4.383524531024534 8 32 2.586249398749395 8 33 4.566431277056267 8 34 4.319051827801815 8 35 4.149847282347288 8 36 5.011094877344874 8 37 5.252371933621937 8 38 5.97922438672439 8 39 5.399445947570953 8 40 6.560402837902846 8 41 7.486481782106797 8 42 6.791614959114963 8 43 6.3458814333814395 8 44 7.5366456228956125 8 45 7.284870731120744 8 46 7.8481000481000365 8 47 7.768695586820588 8 48 8.775527898027896 8 49 8.726741221741218 8 50 8.566471861471872 8 51 8.036562950937963 8 52 8.454294733044755 8 53 7.622207792207792 8 54 9.860635221260239 8 55 10.004038299663303 8 56 7.390177068302066 9 1 0.8849260461760551 9 2 1.447000060125056 9 3 -0.4854025372775297 9 4 0.8507831289081196 9 5 0.8736111111111242 9 6 -0.042217412217397055 9 7 0.832251382876386 9 8 0.8964420995671034 9 9 0.8700673400673367 9 10 0.22837812650311662 9 11 -0.6174639249639213 9 12 -1.1320499639249562 9 13 -1.3031517556517613 9 14 -0.9649062049061996 9 15 -0.24772366522366696 9 16 0.8859623015873055 9 17 0.6841080447330512 9 18 1.392568241943259 9 19 1.3803255772005798 9 20 0.4890981240981489 9 21 2.4210410654160555 9 22 1.4878126503126623 9 23 2.0790241702741596 9 24 2.64212061087062 9 25 1.9429731842232059 9 26 3.3305979437229496 9 27 3.1815572390572413 9 28 3.566463744588752 9 29 2.1252266714766677 9 30 2.9536961880711843 9 31 4.158524531024533 9 32 3.4112493987493977 9 33 4.291431277056269 9 34 3.5190518278018175 9 35 3.949847282347285 9 36 1.386094877344874 9 37 1.6523719336219358 9 38 1.2042243867243911 9 39 1.0744459475709505 9 40 1.6854028379028456 9 41 -1.3885182178932034 9 42 0.34161495911495976 9 43 0.7708814333814367 9 44 1.2366456228956153 9 45 0.4098707311207441 9 46 1.7981000481000393 9 47 3.9686955868205906 9 48 3.8505278980278987 9 49 3.526741221741215 9 50 3.7414718614718687 9 51 1.0865629509379602 9 52 0.5792947330447547 9 53 1.6722077922077894 9 54 1.3856352212602374 9 55 1.979038299663305 9 56 1.4901770683020672 10 1 0.46447150072150833 10 2 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4.215149410774416 117 56 2.2123992905242886 118 1 0.8099260461760522 118 2 1.647000060125059 118 3 1.664597462722469 118 4 1.200783128908121 118 5 1.6486111111111228 118 6 1.4827825877826015 118 7 1.2572513828763832 118 8 1.3714420995671048 118 9 1.6450673400673352 118 10 1.1283781265031152 118 11 1.8825360750360787 118 12 1.9429500360750467 118 13 2.1968482443482387 118 14 1.1600937950938004 118 15 1.3272763347763359 118 16 1.360962301587307 118 17 2.059108044733051 118 18 2.267568241943259 118 19 0.4053255772005784 118 20 2.3890981240981475 118 21 1.7460410654160583 118 22 0.31281265031266514 118 23 1.054024170274161 118 24 0.5421206108706187 118 25 1.3929731842232087 118 26 0.7805979437229524 118 27 1.9565572390572399 118 28 0.6914637445887521 118 29 1.100226671476669 118 30 1.8286961880711843 118 31 2.58352453102453 118 32 2.1862493987493963 118 33 3.466431277056266 118 34 3.0190518278018175 118 35 4.149847282347288 118 36 4.311094877344871 118 37 5.052371933621934 118 38 4.379224386724388 118 39 3.8994459475709533 118 40 3.660402837902847 118 41 5.086481782106798 118 42 2.391614959114957 118 43 5.3458814333814395 118 44 6.0366456228956125 118 45 4.8848707311207455 118 46 6.548100048100039 118 47 6.068695586820592 118 48 5.775527898027896 118 49 5.926741221741214 118 50 6.96647186147187 118 51 7.036562950937963 118 52 8.554294733044756 118 53 8.122207792207792 118 54 8.360635221260239 118 55 9.004038299663303 118 56 6.090177068302069 119 1 -0.11507395382394492 119 2 0.5720000601250561 119 3 -1.8604025372775297 119 4 -0.7742168710918804 119 5 0.4986111111111242 119 6 0.20778258778260295 119 7 0.457251382876386 119 8 0.021442099567103412 119 9 -0.5049326599326633 119 10 -2.6466218734968834 119 11 -2.9924639249639213 119 12 -3.757049963924956 119 13 -2.9281517556517613 119 14 -2.7149062049061996 119 15 -2.997723665223667 119 16 -2.9890376984126945 119 17 -4.440891955266949 119 18 -2.857431758056741 119 19 -3.61967442279942 119 20 -3.385901875901851 119 21 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120 3 -1.7354025372775297 120 4 -1.6992168710918776 120 5 -2.051388888888873 120 6 -1.2172174122173942 120 7 -0.24274861712361684 120 8 -0.5285579004328937 120 9 -0.9549326599326662 120 10 -0.2716218734968834 120 11 -0.11746392496392133 120 12 -0.5570499639249533 120 13 -1.8031517556517613 120 14 -0.3399062049061996 120 15 0.3272763347763359 120 16 -0.33903769841269593 120 17 -0.5408919552669502 120 18 0.1675682419432576 120 19 -1.194674422799423 120 20 -0.21090187590185394 120 21 0.7460410654160583 120 22 -0.2871873496873363 120 23 1.054024170274161 120 24 -1.157879389129377 120 25 -1.3070268157767941 120 26 -2.1194020562770532 120 27 -1.0434427609427601 120 28 -2.608536255411245 120 29 -1.2997733285233295 120 30 -1.0713038119288143 120 31 -0.7164754689754673 120 32 -1.0137506012505995 120 33 0.46643127705626597 120 34 0.5190518278018175 120 35 -1.1501527176527162 120 36 -1.988905122655126 120 37 -1.147628066378065 120 38 -1.2207756132756096 120 39 -0.900554052429051 120 40 -1.0395971620971558 120 41 -0.5135182178932034 120 42 -0.10838504088503953 120 43 -1.5541185666185626 120 44 -0.463354377104384 120 45 -0.015129268879256585 120 46 0.048100048100039317 120 47 1.2686955868205914 120 48 0.8755278980278973 120 49 1.026741221741215 120 50 -1.1335281385281313 120 51 1.2365629509379588 120 52 0.6542947330447539 120 53 2.322207792207788 120 54 2.860635221260239 120 55 3.2040382996633063 120 56 1.8901770683020658 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 28.0 5 2.0 6 3.0 7 3.0 8 15.0 9 17.0 10 15.0 11 21.0 12 25.0 13 38.0 14 39.0 15 40.0 16 48.0 17 63.0 18 61.0 19 58.0 20 69.0 21 101.0 22 83.0 23 116.0 24 150.0 25 144.0 26 157.0 27 121.0 28 159.0 29 187.0 30 220.0 31 264.0 32 333.0 33 405.0 34 544.0 35 705.0 36 933.0 37 1454.0 38 1904.0 39 1091.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 96.71107410491257 1.5507910074937552 0.5203996669442131 1.2177352206494587 8 97.43136439267886 1.559900166389351 0.5407653910149751 0.46797004991680535 9 94.78993344425956 2.589434276206323 1.1647254575707155 1.4559068219633942 10 28.6085690515807 37.302412645590685 24.001663893510816 10.087354409317802 11 35.62811980033278 36.98003327787022 15.235024958402663 12.156821963394343 12 45.133111480865225 14.56946755407654 26.37271214642263 13.924708818635606 13 21.724209650582363 24.97920133111481 22.784941763727122 30.511647254575706 14 27.339850249584025 32.48752079866888 20.798668885191347 19.37396006655574 15 36.85524126455907 20.684276206322796 19.77953410981697 22.680948419301163 16 18.75 28.005407653910147 30.199667221297837 23.044925124792012 17 33.44425956738768 20.736272878535775 17.876455906821963 27.943011647254572 18 32.633111480865225 23.648086522462563 22.795341098169715 20.923460898502498 19 26.08153078202995 24.074459234608987 30.688435940099833 19.155574043261232 20 22.296173044925123 34.32820299500832 17.533277870216306 25.84234608985025 21 21.235440931780367 25.925540765391013 30.2932612312812 22.54575707154742 22 21.641014975041596 24.147254575707155 20.861064891846922 33.35066555740433 23 24.54242928452579 34.08901830282861 23.471297836938437 17.897254575707155 24 20.486688851913478 37.988768718802 20.89226289517471 20.632279534109816 25 21.317103620474406 24.06367041198502 22.284644194756552 32.33458177278402 26 29.194421315570356 22.918401332223148 22.356369691923398 25.530807660283095 27 18.30296720458095 23.040083289953152 24.85163977095263 33.80530973451327 28 18.427083333333332 35.364583333333336 27.0 19.208333333333332 29 26.750729470612754 26.83409754064193 28.063776573572323 18.35139641517299 30 18.394160583941606 36.32950990615224 28.72784150156413 16.548488008342023 31 25.94910304547351 28.09762202753442 30.538172715894866 15.415102211097203 32 16.736227045075125 27.83806343906511 40.546744574290486 14.878964941569281 33 22.55505688341509 25.62363010124204 32.794071600041754 19.027241415301116 34 20.519778728733954 26.959607556622483 34.4118567999165 18.108756914727064 35 16.35167589015349 35.19891406494727 35.10493891615328 13.344471128745955 36 19.133159268929504 27.822454308093995 33.608355091383814 19.43603133159269 37 15.454353784524564 27.54585705249842 41.37676576006747 15.62302340290955 38 19.539989545216937 27.14061683220073 39.14270778881338 14.176685833768948 39 16.23627809722948 26.92106638787245 38.29587036069002 18.546785154208052 40 15.969382405368565 28.184963825102233 41.3966656181189 14.448988151410298 41 15.52788531966098 29.214188552893166 38.93481217955425 16.3231139478916 42 15.457875457875458 27.26321297749869 39.84301412872841 17.435897435897434 43 16.95110459637734 26.813946183645694 40.60307821170558 15.631871008271386 44 16.08171817705605 27.061288632792035 40.806705081194345 16.05028810895757 45 17.30386508850948 27.92500261862365 39.14318634125903 15.627945951607835 46 15.306443163960187 28.789942378208487 38.40754321634364 17.49607124148769 47 16.340211584791035 29.52760029328585 39.12223735204776 15.009950769875354 48 17.26452695615691 28.9595133207468 37.57079924480806 16.20516047828823 49 19.32878867330886 29.564761405348715 37.933927635028844 13.172522286313582 50 16.65268456375839 29.299496644295303 39.27223154362416 14.775587248322147 51 15.681341719077569 31.79245283018868 38.35429769392034 14.171907756813418 52 16.680645837701825 30.509540784231493 38.60348081358775 14.206332564478927 53 16.551290119572055 31.05726872246696 37.81204111600587 14.579400041955108 54 16.182485579444155 30.93864708966964 37.08442579968537 15.794441531200839 55 17.40954378605139 29.585736759307814 36.26638699528055 16.738332459360254 56 17.661248033560568 32.59570005243839 33.87519664394337 15.867855270057682 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 4.5 7 9.0 8 8.5 9 8.0 10 5.0 11 2.0 12 8.5 13 14.0 14 13.0 15 19.0 16 25.0 17 43.5 18 62.0 19 55.5 20 49.0 21 107.5 22 161.5 23 157.0 24 164.5 25 172.0 26 227.0 27 282.0 28 344.5 29 497.0 30 587.0 31 573.5 32 560.0 33 510.5 34 461.0 35 400.5 36 340.0 37 468.0 38 400.5 39 205.0 40 184.5 41 164.0 42 179.5 43 195.0 44 186.5 45 178.0 46 207.0 47 270.5 48 305.0 49 267.5 50 230.0 51 268.5 52 307.0 53 329.5 54 403.5 55 455.0 56 503.5 57 552.0 58 505.0 59 458.0 60 513.0 61 568.0 62 482.5 63 350.0 64 303.0 65 275.5 66 248.0 67 214.0 68 180.0 69 151.5 70 123.0 71 102.0 72 70.0 73 59.0 74 52.0 75 45.0 76 36.5 77 28.0 78 23.0 79 13.0 80 8.0 81 6.0 82 4.0 83 3.0 84 2.0 85 2.0 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.08319467554076539 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.04159733777038269 26 0.08319467554076539 27 0.11439267886855242 28 0.16638935108153077 29 0.20798668885191346 30 0.27038269550748756 31 0.2911813643926789 32 0.33277870216306155 33 0.36397670549084854 34 0.36397670549084854 35 0.40557404326123125 36 0.4263727121464226 37 1.3519134775374375 38 0.5303660565723793 39 0.5303660565723793 40 0.8215474209650582 41 0.6135607321131448 42 0.6343594009983361 43 0.6759567387687188 44 0.7383527454242929 45 0.7175540765391014 46 0.7383527454242929 47 0.7175540765391014 48 0.8527454242928452 49 0.8423460898502495 50 0.8319467554076538 51 0.7903494176372712 52 0.8111480865224625 53 0.8527454242928452 54 0.8423460898502495 55 0.8423460898502495 56 0.8423460898502495 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 9616.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.20549084858568 #Duplication Level Percentage of deduplicated Percentage of total 1 79.12212504049239 50.80074875207987 2 12.212504049238742 15.682196339434276 3 3.952057013281503 7.612312811980034 4 1.830255911888565 4.700499168053245 5 0.9718172983479106 3.119800332778702 6 0.5344995140913509 2.0590682196339434 7 0.32393909944930355 1.4559068219633942 8 0.16196954972465177 0.8319467554076538 9 0.1943634596695821 1.123128119800333 >10 0.6154842889536767 6.083610648918469 >50 0.06478781988986071 2.8806156405990015 >100 0.016196954972465177 3.6501663893510816 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 351 3.6501663893510816 No Hit ACACCCGGGGAGCTGCCCAGGTGTTCCTAGGAGCTGGGCTCTGGTTTTGGAGAACA 86 0.894342762063228 No Hit ACACCCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.7799500831946755 No Hit ACACCCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 63 0.6551580698835274 No Hit ACACCCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA 53 0.5511647254575708 No Hit ACACCCGGGTGTAGCACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.509567387687188 No Hit ACACCCGGGCACACACTCACCTGTGTTGTACAATGACACAGTTTGGCCACACGCCC 28 0.2911813643926789 No Hit ACACCCGGGTGTAGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.2911813643926789 No Hit ACACCCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA 25 0.25998336106489184 No Hit ACACCCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG 24 0.24958402662229617 No Hit ACACCCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.21838602329450912 No Hit ACACCCGGGTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.20798668885191346 No Hit ACACCCGGGTGTAGTACTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.20798668885191346 No Hit ACACCCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCTTCTG 18 0.18718801996672213 Illumina Single End Adapter 2 (96% over 30bp) ACACCCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.18718801996672213 No Hit ACACCCGGGCAATTAAGTGGTAGGGGAGCATTGTGTAAGCCTGTGAAGGTGTGTTG 17 0.17678868552412644 No Hit ACACCCGGGTGTAGCACTGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.16638935108153077 No Hit ACACCCGGGTGTAGCATTGCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.16638935108153077 No Hit ACACCCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.1559900166389351 No Hit ACACCCGGGTACTAAATAGTCATTCTTTTCTTGGTATTTCTTCCTTGGAAAAATCA 14 0.14559068219633944 No Hit ACACCCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG 13 0.13519134775374378 No Hit ACACCCGGGGAGTGGTACAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.13519134775374378 No Hit ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCG 13 0.13519134775374378 Illumina Single End Adapter 2 (95% over 23bp) ACACCCGGGAAGTACGCAAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.13519134775374378 No Hit ACACCCAGGAACATGTTCAACCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.12479201331114809 No Hit ACACCCGGGAAGCTGGACAACGCAGAGTCGACTTTTTTTTTCTTTTTTTTTTTTTT 12 0.12479201331114809 No Hit ACACCCGGGGGGGCGTTCAAGATGTCGAAGCGAGGACGCGGTGGGTCCTCCGGGGC 12 0.12479201331114809 No Hit ACACCCGGGATGGTATCAACGAGAGCCGACTTTTGTTTTTTTTTTTTTTTTTTTTT 12 0.12479201331114809 No Hit ACACCCGGGGACACCACCCACCCAGAGTGGAGAAGCCCAGCCAGTCGCTGTCAGGA 11 0.11439267886855242 No Hit ACACCCGGGAAGCAGTGGTATCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTT 11 0.11439267886855242 No Hit ACACCCGGGTGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.11439267886855242 No Hit ACACCCGGGAGCCCAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11 0.11439267886855242 No Hit ACACCCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.11439267886855242 No Hit ACACCCGGGGATGACACATGAGCAAAAGCCCACTTCGCCATCATATTCGTAGGAGT 11 0.11439267886855242 No Hit ACACCCGGGTGGACGCAGAGTCGACTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTT 10 0.10399334442595673 No Hit ACACCCGGGAAGAGCCTCTGGCATCTCTTTCTGCAAAGGCACCTGAATGTGTCTGC 10 0.10399334442595673 No Hit ACACCCGAGCTGTACGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10399334442595673 No Hit ACACCCGGGGAGTCGACTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10399334442595673 No Hit ACACCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTACACCCGGGTGTAGCA 10 0.10399334442595673 No Hit ACACCCGGGATGTTCAACGCAGAGTCGACCTTTTTTTTTTTTTTTTTTTTTTTTTT 10 0.10399334442595673 No Hit ACACCCGGCAGCCGCGAAGCAGCAACCATGCGTGAGTGCATCTCCATCCATGTTGG 10 0.10399334442595673 No Hit ACACCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 10 0.10399334442595673 No Hit ACACCCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC 10 0.10399334442595673 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.05199667221297837 0.0 0.0 0.0 0.0 11 0.06239600665557404 0.0 0.0 0.0 0.0 12 0.08319467554076539 0.0 0.0 0.0 0.0 13 0.17678868552412647 0.0 0.0 0.0 0.0 14 0.1975873544093178 0.0 0.0 0.0 0.0 15 0.2079866888519135 0.0 0.0 0.0 0.0 16 0.24958402662229617 0.0 0.0 0.0 0.0 17 0.25998336106489184 0.0 0.0 0.0 0.0 18 0.2703826955074875 0.0 0.0 0.0 0.0 19 0.28078202995008317 0.0 0.0 0.0 0.0 20 0.3119800332778702 0.0 0.0 0.0 0.0 21 0.33277870216306155 0.0 0.0 0.0 0.0 22 0.3431780366056572 0.0 0.0 0.0 0.0 23 0.3431780366056572 0.0 0.0 0.0 0.0 24 0.40557404326123125 0.0 0.0 0.0 0.0 25 0.415973377703827 0.0 0.0 0.0 0.0 26 0.415973377703827 0.0 0.0 0.0 0.0 27 0.42637271214642264 0.0 0.0 0.0 0.0 28 0.540765391014975 0.0 0.0 0.0 0.0 29 0.5823627287853578 0.0 0.0 0.0 0.0 30 0.6343594009983361 0.0 0.0 0.0 0.0 31 0.6343594009983361 0.0 0.0 0.0 0.0 32 0.6447587354409318 0.0 0.0 0.0 0.0 33 0.6447587354409318 0.0 0.0 0.0 0.0 34 0.6551580698835274 0.0 0.0 0.0 0.0 35 0.7279534109816972 0.0 0.0 0.0 0.0 36 0.7383527454242929 0.0 0.0 0.0 0.0 37 0.7591514143094842 0.0 0.0 0.0 0.0 38 0.7695507487520798 0.0 0.0 0.0 0.0 39 0.7903494176372712 0.0 0.0 0.0 0.0 40 0.8215474209650583 0.0 0.0 0.0 0.0 41 0.8423460898502496 0.0 0.0 0.0 0.0 42 0.8423460898502496 0.0 0.0 0.0 0.0 43 0.8423460898502496 0.0 0.0 0.0 0.0 44 0.8423460898502496 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGGTG 40 2.2773747E-9 49.895836 6 CGGGGTA 20 4.019596E-4 49.895836 6 ACACCCC 20 4.019596E-4 49.895836 1 CGGGTGC 20 4.019596E-4 49.895836 6 CGGGAGA 20 4.019596E-4 49.895836 6 CCGGGTG 160 0.0 49.895836 5 CCGGGTA 35 4.6678906E-8 49.895836 5 CCGGGGT 70 0.0 49.895836 5 ATCCCCA 15 0.008247926 49.895832 24 GGAGAAA 15 0.008247926 49.895832 20 GAGAAAG 15 0.008247926 49.895832 21 TCAAGAT 15 0.008247926 49.895832 19 AATTCGT 15 0.008247926 49.895832 13 CGGGGGC 15 0.008247926 49.895832 6 CGGGGCT 25 1.959521E-5 49.895832 6 CCCGGGT 220 0.0 49.895832 4 ACCCGGC 15 0.008247926 49.895832 3 TAGCATT 15 0.008247926 49.895832 12 CGGGCGT 15 0.008247926 49.895832 6 CGTGGAG 15 0.008247926 49.895832 17 >>END_MODULE