FastQCFastQC Report
Fri 17 Jun 2016
SRR1526544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences656890
Sequences flagged as poor quality0
Sequence length64
%GC60

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC71051.0816118375983803No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC34220.5209395789249341No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC24690.37586201647155537No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT23040.35074365571100186No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC22020.33521594178629605No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC20230.3079663261733319No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC19720.300202469210979No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC18940.2883283350332628No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC13470.20505716329979143No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT11640.17719861772899573No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT10690.1627365312304952No Hit
CGGCAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG10570.1609097413570004No Hit
CGGCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT10200.1552771392470581No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC10180.15497267426814232No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC9370.1426418426220524No Hit
CGGCAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT8760.1333556607651205No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8460.1287886860813835No Hit
CGGCAGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG8410.12802752363409398No Hit
CGGCAGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC8020.12209045654523588No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT6710.10214800042625098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGG150.004628259758.18295353
CATATCG150.00462966358.17849752
CGGCAGG666500.056.58791
GGCAGGG666050.056.161592
GCAGGGG653450.056.1249353
CAGGGGA134300.056.0459674
CAGGGGG453750.055.9722144
AGGGGGG259400.055.4809535
AGGGGAT28700.054.5081675
AGGGGGA95100.054.366735
AGGGGGC69550.053.716985
AGGGGAG58050.052.801945
AGGGGAC25350.051.4451945
AGGGGGT40900.051.3286135
CAGGGGC41600.051.229534
CAGGGGT31650.051.1635364
TTCATAC850.051.1030922
AGGGGTG15950.050.581275
AGGGGAA31950.049.868585
CGATACT351.0961812E-649.66950226
Produced by FastQC (version 0.11.5)