FastQCFastQC Report
Fri 17 Jun 2016
SRR1526543_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526543_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences637778
Sequences flagged as poor quality0
Sequence length64
%GC59

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC58330.914581562863567No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC28770.451097403798814No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC22790.35733437026676995No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT19730.3093552929075634No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC18380.2881880528961488No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC16590.26012186058471753No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC16010.251027787098332No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC15930.2497734321346926No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT12460.1953657855868343No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC11690.18329261906180522No Hit
CGGCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT9090.14252608274352518No Hit
CGGCAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG8830.13844942911169716No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT8640.13547033607305362No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC8310.13029612184804118No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC8030.12590587947530332No Hit
CGGCAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT7330.1149302735434587No Hit
CGGCAGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6970.10928567620708146No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6860.1075609381320773No Hit
CGGCAGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG6530.10238672390706484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA201.9720521E-458.222235
GATAATC150.00465934658.08445742
TACCGAC201.9968348E-458.07529440
ACTAGTA150.00472368257.88361412
AAGATAG150.004735450357.84724417
CGGCAGG648450.056.3359531
GCAGGGG631600.055.968053
GGCAGGG644700.055.964432
CAGGGGG438200.055.8365174
CAGGGGA129500.055.5885774
AGGGGGG250200.055.2461435
AGGGGGA90600.054.490555
AGGGGAT28400.053.8077285
AGGGGGC67500.053.666235
AGGGGTG15400.053.0851485
AGGGGAG55250.053.0167355
CAGGGGT30500.052.660174
AGGGGGT40300.051.753475
CAGGGGC40150.051.7309764
AGGGGAC23950.050.6583565
Produced by FastQC (version 0.11.5)