##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526543_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 637778 Sequences flagged as poor quality 0 Sequence length 64 %GC 59 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.284089448052455 39.0 38.0 39.0 35.0 40.0 2 37.78423056298587 39.0 38.0 39.0 35.0 40.0 3 37.90427703683727 39.0 38.0 39.0 35.0 40.0 4 37.775744851656846 39.0 38.0 39.0 35.0 40.0 5 37.90406066060604 39.0 38.0 39.0 35.0 40.0 6 38.04366096039688 39.0 38.0 39.0 35.0 40.0 7 38.232745563503286 39.0 38.0 39.0 36.0 40.0 8 38.08976164119804 39.0 38.0 39.0 36.0 40.0 9 38.24631454832246 39.0 38.0 39.0 36.0 40.0 10 37.46486865335587 39.0 38.0 39.0 33.0 40.0 11 36.84730893821988 39.0 35.0 39.0 32.0 40.0 12 36.72524452082072 39.0 35.0 39.0 32.0 40.0 13 36.72362953880504 39.0 35.0 39.0 32.0 40.0 14 36.68919279122203 39.0 36.0 39.0 32.0 40.0 15 36.62393340629498 39.0 36.0 39.0 32.0 40.0 16 36.168817362781404 38.0 35.0 39.0 30.0 40.0 17 35.46744948869356 38.0 35.0 39.0 29.0 40.0 18 35.82778647115454 38.0 35.0 39.0 30.0 40.0 19 35.62666006039719 38.0 35.0 39.0 29.0 40.0 20 35.53892890629655 38.0 35.0 39.0 29.0 40.0 21 35.344963294437875 38.0 35.0 39.0 29.0 40.0 22 35.20878111192296 38.0 34.0 39.0 29.0 40.0 23 35.158815449890085 38.0 34.0 39.0 28.0 40.0 24 35.08545136395423 38.0 34.0 39.0 28.0 40.0 25 34.925831558943706 38.0 33.0 39.0 28.0 40.0 26 35.17693931117097 38.0 35.0 39.0 28.0 40.0 27 34.66697502892856 38.0 34.0 39.0 26.0 40.0 28 34.49176359172 38.0 33.0 39.0 26.0 40.0 29 34.37773488580666 38.0 33.0 39.0 26.0 40.0 30 34.06855049876289 37.0 33.0 39.0 25.0 40.0 31 33.648075035513926 37.0 33.0 39.0 23.0 40.0 32 33.973918824418526 38.0 33.0 39.0 24.0 40.0 33 33.79693874671124 37.0 33.0 39.0 24.0 40.0 34 32.80683717531806 36.0 31.0 39.0 20.0 40.0 35 32.92741831797271 36.0 32.0 39.0 20.0 40.0 36 32.24118737240858 36.0 31.0 39.0 14.0 40.0 37 32.032417236091554 36.0 31.0 39.0 13.0 40.0 38 31.73532796678468 36.0 30.0 39.0 13.0 39.0 39 31.371746908798986 36.0 30.0 39.0 8.0 40.0 40 31.406404736444344 36.0 30.0 39.0 6.0 39.0 41 30.952983640075388 35.0 30.0 39.0 4.0 39.0 42 30.703044005908012 35.0 29.0 39.0 4.0 39.0 43 30.64152416671632 35.0 29.0 38.0 4.0 39.0 44 30.45989200003763 35.0 29.0 38.0 4.0 39.0 45 30.074596489687636 35.0 28.0 38.0 4.0 39.0 46 29.454186252896775 35.0 28.0 38.0 4.0 39.0 47 29.232424135043857 34.0 27.0 38.0 4.0 39.0 48 28.913540448243747 34.0 26.0 38.0 4.0 39.0 49 28.8181561609212 34.0 26.0 38.0 4.0 39.0 50 28.37799359651791 34.0 25.0 38.0 4.0 39.0 51 27.682472584504325 33.0 23.0 37.0 4.0 39.0 52 27.580317916265535 33.0 23.0 37.0 4.0 39.0 53 27.343476883805963 33.0 22.0 37.0 4.0 39.0 54 27.177356384196383 33.0 21.0 36.0 4.0 39.0 55 27.05344649705697 33.0 20.0 37.0 4.0 39.0 56 26.491506448952457 33.0 12.0 37.0 4.0 39.0 57 25.957812906685398 32.0 9.0 36.0 4.0 39.0 58 25.520557309910345 32.0 4.0 36.0 4.0 39.0 59 25.231600964598968 32.0 4.0 36.0 4.0 38.0 60 25.079704850277057 32.0 4.0 36.0 4.0 39.0 61 24.44625716158287 31.0 4.0 36.0 4.0 38.0 62 23.78719554453117 31.0 4.0 35.0 4.0 38.0 63 23.853803361044125 31.0 4.0 35.0 4.0 38.0 64 23.827182812828287 31.0 4.0 35.0 4.0 38.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 0.0323535939220676 2 2 0.4488415688826777 2 3 0.1054254118056761 2 4 0.49083348497370594 2 5 0.1362800606930037 2 6 0.27375951476464877 2 7 0.3173482790139346 2 8 0.5369242893113153 2 9 0.16812912431311844 2 10 0.4031309483080605 2 11 -0.2687967851626709 2 12 0.023011681662012506 2 13 0.08761404662408268 2 14 -0.3134226127212898 2 15 0.2051244662340821 2 16 0.1842264108079661 2 17 0.4717420312790708 2 18 0.12356595596475728 2 19 -0.1466269119505128 2 20 0.168298539922489 2 21 0.10691066481575007 2 22 -0.3043569943708633 2 23 0.2382826775781055 2 24 0.13447552822980668 2 25 0.2819277360223964 2 26 0.5246813938134309 2 27 0.6918467589389508 2 28 0.9863296979198566 2 29 0.7223565056691115 2 30 0.6232534469057782 2 31 0.8133638214211345 2 32 0.688202853323368 2 33 0.09988858178009252 2 34 0.5678390031552141 2 35 0.10492765850975871 2 36 -0.9468451468500056 2 37 -0.89271059225808 2 38 -1.1395161472625048 2 39 -4.310764461285995 2 40 -1.1360492847168615 2 41 -1.540479260798417 2 42 -1.2523875492385521 2 43 -1.8000226725103623 2 44 -0.9690041556839297 2 45 -1.4747825994471242 2 46 -0.5533543195674326 2 47 -0.6682656674897878 2 48 -0.18162027466944863 2 49 -0.4428495194059323 2 50 -0.3542763441182686 2 51 -0.06787896561001361 2 52 -0.1495836265359607 2 53 0.0922007697778362 2 54 -0.10241741519105574 2 55 -0.28015909439358566 2 56 -0.05577397040985588 2 57 -0.15779561266871767 2 58 0.05993045169068267 2 59 -0.03980021145789081 2 60 0.10637842678723786 2 61 -0.24557701348259897 2 62 -0.4462235541266537 2 63 -0.1430874798010997 2 64 -0.4949663671312088 3 1 0.07246795885015445 3 2 0.25820158736589605 3 3 -0.0859019144809281 3 4 0.4959885707474001 3 5 0.02363060652561444 3 6 0.17566848490269393 3 7 0.3129960581394471 3 8 0.5353763195775869 3 9 0.2738704747434255 3 10 0.04482216300222319 3 11 -0.3060982349402934 3 12 -0.22232130511093118 3 13 -0.33866678281044216 3 14 0.14324867475055214 3 15 0.18051579067236645 3 16 0.07853126842940839 3 17 -0.11389592863544351 3 18 -0.14731141941790327 3 19 0.020623131369532643 3 20 0.07641960501808143 3 21 0.1325070374836912 3 22 0.0563979296746524 3 23 -0.1767060592092804 3 24 -0.38970283484995605 3 25 -0.06932854538464284 3 26 0.1502719904748986 3 27 0.557774096777564 3 28 0.764178713110752 3 29 0.5076074731501947 3 30 0.2143555089400877 3 31 0.6018031444731804 3 32 0.3907905042215347 3 33 0.26195177129086744 3 34 0.7424159106813519 3 35 -0.2712260987824493 3 36 -4.43500722159153 3 37 -3.8102407718918805 3 38 -4.414722639493778 3 39 -18.87649252690143 3 40 -6.01977827449333 3 41 -5.147214084341417 3 42 -5.256347001673138 3 43 -5.568699389428286 3 44 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4 21 0.11163738308926696 4 22 -0.03975460168965128 4 23 0.151149203498818 4 24 -0.15278696297357186 4 25 0.2244447428251135 4 26 0.6012941078645682 4 27 0.8714855109914907 4 28 0.7582391513563991 4 29 0.8021242748457453 4 30 0.6236639559369763 4 31 -0.21889045960161724 4 32 -0.7752266564115615 4 33 -0.6403061167889916 4 34 -0.4070730306238133 4 35 -4.985759650896764 4 36 -0.9743023366224683 4 37 -1.1629310942519808 4 38 -1.123248260797574 4 39 -0.6958102037208995 4 40 -0.4511442882355112 4 41 0.2979795710101705 4 42 0.4627074542800962 4 43 0.7278684839522249 4 44 0.565724908355481 4 45 0.6218864129854325 4 46 0.12169846016515251 4 47 0.3625225098501126 4 48 0.28182096389494404 4 49 0.25340898399542766 4 50 0.4636922226187714 4 51 0.22857892789221168 4 52 0.3106204550966929 4 53 0.07999105830704067 4 54 0.2119152144125067 4 55 0.2712249074830275 4 56 0.2843151686940608 4 57 0.3369967870497881 4 58 0.43839632079694724 4 59 0.6896508793232492 4 60 0.3732444835547746 4 61 0.7978665708476846 4 62 0.5934081606282149 4 63 0.6802646196593756 4 64 0.7265677637625281 5 1 0.1937152208009394 5 2 0.6052960339578277 5 3 0.28731459541899795 5 4 0.58639788226224 5 5 0.22926561919580024 5 6 0.3679976521062329 5 7 0.1914567376051579 5 8 0.4523973220257247 5 9 -0.03160562369749442 5 10 0.3285656668462309 5 11 -1.83655393223016 5 12 -1.8078901751019103 5 13 -1.3044873385807492 5 14 -1.0848490789753455 5 15 -0.8063845228611157 5 16 -18.387568460986902 5 17 -2.5800557877601236 5 18 -2.676994020457286 5 19 -2.9177439175502826 5 20 -2.518203081061891 5 21 -2.3108464822517405 5 22 -2.371038397259163 5 23 -2.5316259578477727 5 24 -2.4254714213723148 5 25 -1.9462773921827363 5 26 0.9005876714438443 5 27 1.5970044365104172 5 28 1.4906916195231545 5 29 1.2384926683569972 5 30 1.0352634675364882 5 31 0.3503664739410297 5 32 0.43382619549843326 5 33 0.1906637070379773 5 34 -0.15763992404784943 5 35 -4.263581035861005 5 36 -0.5053332105104822 5 37 -1.0601140700063851 5 38 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-0.11760345932759719 119 19 0.02398778070315899 119 20 -0.04804328916296896 119 21 0.2554114144409354 119 22 0.07478843291549708 119 23 0.030276680576605486 119 24 0.05198677260761997 119 25 -0.28937661180368934 119 26 0.08310718091988178 119 27 0.3622515565401443 119 28 0.10623974289736537 119 29 0.18507014884752238 119 30 -0.08461761756198882 119 31 0.2131089488574176 119 32 -0.12604002523735147 119 33 0.4301434543438134 119 34 0.5494132160487695 119 35 0.33897563452175206 119 36 0.38135575270021604 119 37 0.19064772858150292 119 38 0.3042169861707755 119 39 0.27989520887892283 119 40 0.04685926101027249 119 41 -0.21136381851955477 119 42 -0.15225261670482126 119 43 -0.4530661507712317 119 44 -0.41837447052650845 119 45 -0.438305837827933 119 46 -0.09806196574434267 119 47 -0.28946506778963865 119 48 0.07718032503070305 119 49 -0.04111038897114838 119 50 -0.11706494981542193 119 51 0.20047185897768927 119 52 -0.29306188740552486 119 53 -0.22543041462996172 119 54 0.14711781719275052 119 55 0.07982591310266685 119 56 -0.22748311583714198 119 57 -0.10011945074968054 119 58 -6.542559554922889E-4 119 59 -0.051884169478878306 119 60 -0.2604881399294037 119 61 0.5010046956628287 119 62 0.5156205238343681 119 63 0.34220487402198785 119 64 0.4356183405149672 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 697.0 5 90.0 6 327.0 7 245.0 8 780.0 9 796.0 10 1512.0 11 1684.0 12 2178.0 13 2874.0 14 3335.0 15 4091.0 16 5013.0 17 5462.0 18 5883.0 19 7233.0 20 8052.0 21 10483.0 22 13441.0 23 9886.0 24 11545.0 25 11142.0 26 12273.0 27 13899.0 28 15743.0 29 17994.0 30 20757.0 31 24739.0 32 29914.0 33 37787.0 34 47743.0 35 61703.0 36 76379.0 37 84143.0 38 63880.0 39 24064.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 100.0 0.0 0.0 0.0 7 99.21492197042512 0.5773232084870589 0.10332862422405534 0.10442619686376459 8 98.85350498336264 0.7124020074644544 0.2580711962737343 0.1760218128991671 9 97.66329349711029 1.2322469574052415 0.6119684278855653 0.49249111759891373 10 68.73520253128832 20.511055571060776 4.748047126115984 6.005694771534923 11 49.49716045395106 21.495724217517694 12.935535562531165 16.07157976600008 12 44.48271969243216 14.10678950982944 21.028790582302932 20.381700215435465 13 29.51873535932566 13.672312309298848 23.129051174546632 33.67990115682887 14 23.749016115325393 15.792328992219865 35.27308875502134 25.1855661374334 15 34.08583404079698 16.051081752459385 28.938898679174518 20.924185527569115 16 42.05533311690654 16.821850950944828 20.597871175871862 20.52494475627677 17 34.63259939558068 20.10298677342125 24.38431649593783 20.880097335060245 18 39.955909423028075 18.806857558586216 20.91119480446174 20.326038213923965 19 33.114814245709326 27.197865087851884 18.845585768088583 20.841734898350207 20 36.34039777057777 25.167881013841363 18.89143642163305 19.600284793947814 21 31.213525709572927 26.295513485883802 23.047518101910068 19.443442702633202 22 27.773927604903275 19.965567956248098 31.496696342614516 20.76380809623411 23 35.30178212481459 16.963269350777228 25.999172125723994 21.73577639868418 24 37.935203246423356 19.686616030156113 19.90566806951962 22.472512653900914 25 30.921681180610356 17.75668972268458 19.199037389027556 32.122591707677515 26 24.010212707229975 17.313455774994235 28.938408530245262 29.737922987530524 27 23.251857553527387 16.62514334186839 26.364693130586403 33.758305974017816 28 23.466012952830837 25.938326433951243 20.858800324134855 29.73686028908306 29 32.98921003923194 23.31906289198579 20.532637551198544 23.159089517583727 30 31.524738975883835 17.408623888085174 27.43144729959615 23.635189836434837 31 35.769078475984564 16.18826442748859 26.124564755571754 21.918092340955088 32 41.331327448637154 16.035038073596912 22.652259054355184 19.981375423410746 33 39.318844587460404 16.75769269445422 24.702096636331643 19.221366081753732 34 27.74788696709948 18.505462963108673 36.13345272172486 17.613197348066993 35 24.24873512126961 30.3427864582924 28.314265191511833 17.094213228926154 36 26.529084099035536 32.96727498108358 22.276860927714882 18.226779992166005 37 33.529737536855286 26.640591320874645 20.433798934539972 19.395872207730093 38 30.85543847947218 28.86511010360657 20.61685706824567 19.66259434867558 39 34.404660652548166 22.49029951193803 20.60685054068682 22.498189294826982 40 29.71811527156228 18.760739439944107 21.122629455490728 30.398515833002886 41 22.73019231495825 26.14151584992632 22.13715822218864 28.991133612926788 42 21.522114913404465 20.87054081438096 25.09308764183752 32.51425663037706 43 22.314191531470208 16.554002949740955 28.27922323488258 32.85258228390626 44 27.12021369082868 16.360704070961482 25.21545478599418 31.303627452215654 45 28.893612126922402 19.771635467561175 23.565946679229288 27.768805726287134 46 24.892997396637693 23.655454768943716 23.589897945676086 27.861649888742505 47 22.625634495767926 19.888300378688193 28.513030309238385 28.9730348163055 48 25.752440379115555 19.215296981729654 30.979854562071115 24.05240807708368 49 31.896686224357122 16.694324802650137 26.208408388531407 25.200580584461328 50 24.606978769595838 17.44471511877414 27.175264433393075 30.77304167823694 51 22.259831781741934 19.215034014141686 31.11139138728825 27.41374281682813 52 21.38159191506338 19.427217121042247 29.789156484017614 29.402034479876754 53 23.231000954190947 16.991696172984565 34.437145628464854 25.34015724435963 54 21.446605049729314 16.936037077395437 32.41197035953026 29.205387513344995 55 24.306498759309125 16.983504253684607 25.7248142907397 32.98518269626657 56 22.823104727315183 20.825240956917266 22.977878821823623 33.37377549394393 57 28.34703311613524 25.688433807172274 21.403057602196174 24.561475474496316 58 26.02060143824935 32.21145043469477 20.174635544583836 21.593312582472045 59 29.91874050946607 29.33565473240246 20.08867828628975 20.656926471841725 60 36.57775560089185 22.19576435514739 20.10936503224421 21.117115011716553 61 33.852501045224706 18.989484645057466 22.419083830965473 24.73893047875236 62 25.727564684252474 18.261987990310185 26.94541982624614 29.0650274991912 63 23.602107097091853 18.01637450526143 23.248294059349693 35.133224338297026 64 24.32191263752057 18.59129660221928 23.24101595443935 33.845774805820795 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 19.0 11 36.0 12 158.0 13 257.0 14 234.0 15 290.5 16 399.5 17 452.0 18 683.5 19 915.0 20 1253.0 21 1649.5 22 1708.0 23 1874.0 24 2383.0 25 2726.0 26 3191.5 27 4160.0 28 4663.0 29 5043.0 30 5556.5 31 5690.0 32 5271.5 33 4853.0 34 5018.0 35 5041.0 36 4899.0 37 4768.0 38 4737.5 39 4838.0 40 5027.0 41 5463.5 42 5711.0 43 6197.0 44 6683.0 45 6857.5 46 7761.5 47 8491.0 48 8879.0 49 9954.5 50 10642.0 51 11425.5 52 12926.5 53 13644.0 54 14944.5 55 18240.5 56 20236.0 57 24569.5 58 28903.0 59 33229.5 60 40393.5 61 43231.0 62 49798.5 63 64137.0 64 71908.0 65 67623.5 66 53171.0 67 43003.0 68 39231.0 69 35459.0 70 31195.5 71 23288.5 72 19645.0 73 16901.5 74 11941.5 75 9725.0 76 8258.0 77 5616.5 78 4442.0 79 3610.0 80 2254.5 81 1731.0 82 1352.0 83 973.0 84 767.5 85 449.0 86 336.0 87 263.5 88 146.0 89 101.0 90 78.0 91 37.5 92 20.0 93 16.5 94 13.0 95 8.5 96 3.0 97 2.0 98 3.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0010975605931844623 8 0.05597559025240757 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.08231704448883467 16 0.023362361197783555 17 0.12590587947530332 18 0.0 19 0.0 20 0.019442501936410474 21 0.0 22 0.0 23 0.0 24 0.004703831113647696 25 0.0540940578069485 26 0.02210800623414417 27 0.18627171210044874 28 0.1577351366776527 29 0.12496511325257378 30 0.18047032039361657 31 0.21229957759596602 32 0.15444245489809935 33 0.20853651270504783 34 0.363292556344057 35 0.24318806857558586 36 0.32722985113942465 37 0.34243890507355224 38 0.32989535543715837 39 0.6345468172310741 40 0.35592322093267564 41 0.4029615320691526 42 0.4956270050080122 43 0.49327508945118836 44 0.4456096008328917 45 0.4846513990761676 46 0.5039371066421231 47 0.5047210784943977 48 0.4928047063398236 49 0.5087977321262258 50 0.6224736507060451 51 0.5461147922944974 52 0.5661844717127276 53 0.5853133849082283 54 0.571515480308195 55 0.6047558868446388 56 0.6194945576674016 57 0.6190241745560368 58 0.6632401870243251 59 0.6666896631743334 60 0.7033795458607853 61 0.6182402027037621 62 0.6464631893856483 63 0.6444248625697343 64 0.7212541040926467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 637778.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.78731583946721 #Duplication Level Percentage of deduplicated Percentage of total 1 87.07335415086635 48.57588709216164 2 5.248276170145832 5.855744806333498 3 2.2164100384154786 3.709427005285499 4 1.3372104380287075 2.983975242005592 5 0.8735348109369554 2.4366081197254603 6 0.5775969410120025 1.933354978568802 7 0.4501637008956374 1.757939719292986 8 0.35087936943071274 1.5659694563187414 9 0.25838002750407446 1.2972895380882026 >10 1.4354449750497262 14.688027633716722 >50 0.11245995733892782 4.386074788835239 >100 0.06009896866742642 6.2378606162351335 >500 0.0036110968297952952 1.3963598482094037 >1k 0.00232141939058269 2.3361399562736676 >5k 2.5793548784252114E-4 0.839341198949449 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 5833 0.914581562863567 No Hit CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 2877 0.451097403798814 No Hit CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 2279 0.35733437026676995 No Hit CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCAAAGGCCCT 1973 0.3093552929075634 No Hit CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC 1838 0.2881880528961488 No Hit CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC 1659 0.26012186058471753 No Hit CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 1601 0.251027787098332 No Hit CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC 1593 0.2497734321346926 No Hit CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 1246 0.1953657855868343 No Hit CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC 1169 0.18329261906180522 No Hit CGGCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT 909 0.14252608274352518 No Hit CGGCAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG 883 0.13844942911169716 No Hit CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT 864 0.13547033607305362 No Hit CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTCGGAAACCC 831 0.13029612184804118 No Hit CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC 803 0.12590587947530332 No Hit CGGCAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT 733 0.1149302735434587 No Hit CGGCAGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 697 0.10928567620708146 No Hit CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 686 0.1075609381320773 No Hit CGGCAGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG 653 0.10238672390706484 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.002508709927278771 0.0 0.0 0.0 0.0 6 0.002508709927278771 0.0 0.0 0.0 0.0 7 0.002508709927278771 0.0 0.0 0.0 0.0 8 0.002665504297733694 0.0 0.0 0.0 0.0 9 0.0028222986681886173 0.0 0.0 0.0 0.0 10 0.004547036743192772 0.0 0.0 0.0 0.0 11 0.006585363559106774 0.0 0.0 0.0 0.0 12 0.007526129781836313 0.0 0.0 0.0 0.0 13 0.008310101634110929 0.0 0.0 0.0 0.0 14 0.010662017190934776 0.0 0.0 0.0 0.0 15 0.011445989043209393 0.0 0.0 0.0 0.0 16 0.012543549636393855 0.0 0.0 0.0 0.0 17 0.01332752148866847 0.0 0.0 0.0 0.0 18 0.014111493340943087 0.0 0.0 0.0 0.0 19 0.01583623141594724 0.0 0.0 0.0 0.001567943704549232 20 0.01771776386140632 0.0 0.0 0.0 0.001567943704549232 21 0.018501735713680936 0.0 0.0 0.0 0.001567943704549232 22 0.019756090677320322 0.0 0.0 0.0 0.001567943704549232 23 0.02179441749323432 0.0 0.0 0.0 0.001567943704549232 24 0.025243893643242633 0.0 0.0 0.0 0.001567943704549232 25 0.026341454236427097 0.0 0.0 0.0 0.001567943704549232 26 0.027752603570521405 0.0 0.0 0.0 0.001567943704549232 27 0.029163752904615713 0.0 0.0 0.0 0.001567943704549232 28 0.0324564346841691 0.0 0.0 0.0 0.001567943704549232 29 0.034181172759173255 0.0 0.0 0.0 0.001567943704549232 30 0.03606270520463233 0.0 0.0 0.0 0.001567943704549232 31 0.037317060168271715 0.0 0.0 0.0 0.001567943704549232 32 0.03935538698418572 0.0 0.0 0.0 0.001567943704549232 33 0.04029615320691526 0.0 0.0 0.0 0.001567943704549232 34 0.042648068763739104 0.0 0.0 0.0 0.001567943704549232 35 0.0493902266933008 0.0 0.0 0.0 0.001567943704549232 36 0.051898936620579574 0.0 0.0 0.0 0.001567943704549232 37 0.05425085217740342 0.0 0.0 0.0 0.001567943704549232 38 0.05660276773422727 0.0 0.0 0.0 0.001567943704549232 39 0.06005224388423558 0.0 0.0 0.0 0.001567943704549232 40 0.0635017200342439 0.0 0.0 0.0 0.001567943704549232 41 0.06569684122061281 0.0 0.0 0.0 0.001567943704549232 42 0.06851913988880143 0.0 0.0 0.0 0.001567943704549232 43 0.07118464418653513 0.0 0.0 0.0 0.001567943704549232 44 0.07400694285472374 0.0 0.0 0.0 0.001724738075004155 45 0.07682924152291236 0.0 0.0 0.0 0.001724738075004155 46 0.07996512893201083 0.0 0.0 0.0 0.001724738075004155 47 0.08278742760019944 0.0 0.0 0.0 0.001724738075004155 48 0.0865504924911176 0.0 0.0 0.0 0.001724738075004155 49 0.08780484745475699 0.0 0.0 0.0 0.001724738075004155 50 0.09078394049340052 0.0 0.0 0.0 0.001724738075004155 51 0.09376303353204407 0.0 0.0 0.0 0.001724738075004155 52 0.09689892094114252 0.0 0.0 0.0 0.001724738075004155 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCTA 20 1.9720521E-4 58.2222 35 GATAATC 15 0.004659346 58.084457 42 TACCGAC 20 1.9968348E-4 58.075294 40 ACTAGTA 15 0.004723682 57.883614 12 AAGATAG 15 0.0047354503 57.847244 17 CGGCAGG 64845 0.0 56.335953 1 GCAGGGG 63160 0.0 55.96805 3 GGCAGGG 64470 0.0 55.96443 2 CAGGGGG 43820 0.0 55.836517 4 CAGGGGA 12950 0.0 55.588577 4 AGGGGGG 25020 0.0 55.246143 5 AGGGGGA 9060 0.0 54.49055 5 AGGGGAT 2840 0.0 53.807728 5 AGGGGGC 6750 0.0 53.66623 5 AGGGGTG 1540 0.0 53.085148 5 AGGGGAG 5525 0.0 53.016735 5 CAGGGGT 3050 0.0 52.66017 4 AGGGGGT 4030 0.0 51.75347 5 CAGGGGC 4015 0.0 51.730976 4 AGGGGAC 2395 0.0 50.658356 5 >>END_MODULE