FastQCFastQC Report
Fri 17 Jun 2016
SRR1526542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595316
Sequences flagged as poor quality0
Sequence length56
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC95901.6109091642085884No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC53240.8943149520590745No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC30350.5098132756384844No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCTTCCA24580.41288996096190933No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC21050.35359372165370995No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT19770.3320925357289238No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC16200.2721243843605749No Hit
CGGCAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT15960.2680929119996775No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC14840.24927937431548958No Hit
CGGCAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC13430.22559447419521736No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC12810.21517983726289905No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT9930.16680216893213015No Hit
CGGCAGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9340.15689146604492404No Hit
CGGCAGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC9090.15269201566898924No Hit
CGGCAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTCC8780.14748469720283008No Hit
CGGCAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGTCTC8610.14462907094719443No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC8290.1392537744659979No Hit
CGGCAGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC8080.13572623615021265No Hit
CGGCAGGGGGGGACTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC7690.12917509356375437No Hit
CGGCAGGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC7600.12766329142841784No Hit
CGGCAGGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT7440.12497564318781959No Hit
CGGCAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG7390.12413575311263261No Hit
CGGCAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCTCTTC7070.11876045663143607No Hit
CGGCAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA6990.11741663251113695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA150.008048831550.5871735
CGATACA150.00829622150.20123746
TACTTAT150.00829900150.19698340
AACGATA150.008301781550.19272644
TCAATTA150.00830456250.18847745
CACGGTA252.0433878E-550.17571649
GTAGCGT355.32782E-849.94720529
ACGCTTA204.218111E-449.9135326
CGTATCC150.00854353849.82953323
CCGATAC150.00855490849.81276722
CGCATTC150.00858338549.77090520
CGGCAGG603750.048.616871
GGCAGGG602050.048.3450052
GCAGGGG590450.048.253953
CAGGGGG414550.047.9139254
CAGGGGA119350.047.8237424
AGGGGGA85350.047.488685
AGGGGGG231250.047.2448275
AGGGGAT25600.047.0411535
AGGGGTG13600.046.286485
Produced by FastQC (version 0.11.5)