FastQCFastQC Report
Fri 17 Jun 2016
SRR1526541_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526541_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61541
Sequences flagged as poor quality0
Sequence length64
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10541.712679351976731No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.9083375310768431No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4890.7945922230707984No Hit
ACTTATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2380.38673404722055216No Hit
ACTTATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2330.37860938236297753No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.3428608569896492No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090.33961099104661935No Hit
ACTTATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1980.32173672835995515No Hit
ACTTATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1660.26973887327147755No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG1520.2469898116702686No Hit
ACTTATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG1480.2404900797842089No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG1480.2404900797842089No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1470.238865146812694No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.2323654149266343No Hit
ACTTATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1240.2014916884678507No Hit
ACTTATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.173867827952097No Hit
ACTTATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.17061796200906712No Hit
ACTTATGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.14136916852179848No Hit
ACTTATGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.13974423555028356No Hit
ACTTATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.13324450366422386No Hit
ACTTATGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.1169951739490746No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.10887050909149998No Hit
ACTTATGGGGTTCAGACCACCGAAAGCAGTTCCCAGCCACATCCAAAAAAAAAAAAAAAAAAAA640.10399571017695519No Hit
ACTTATGGGTAAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.10399571017695519No Hit
ACTTATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.10237077720544029No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG150.00463667858.0929151
CTATACT150.00463667858.0929150
ACTGACG201.9796158E-458.0929157
AACTGAC201.9796158E-458.0929156
GATGCTT150.00463667858.0929155
TCGAACT201.9796158E-458.0929153
CTGACGG201.9796158E-458.0929158
TCTATAC150.00463667858.0929149
ACATCGA201.9796158E-458.0929150
GAGTTGT150.00463667858.0929157
CTGGCGT150.00463667858.0929152
CGAACTG201.9796158E-458.0929154
ATGCTTT150.00463667858.0929156
AGTTGTA150.00463667858.0929158
ATCGAAC201.9796158E-458.0929152
AACATCG201.9796158E-458.0929149
TTGTAGC150.00465167458.04560541
GCGCCGG150.00465167458.04560546
CGCAGGG303.682926E-758.04560545
TGTAGCG150.00465167458.04560542
Produced by FastQC (version 0.11.5)