FastQCFastQC Report
Fri 17 Jun 2016
SRR1526540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59840
Sequences flagged as poor quality0
Sequence length64
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8161.3636363636363635No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4500.7520053475935828No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.6935160427807486No Hit
ACTTATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG2240.37433155080213903No Hit
ACTTATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.32586898395721925No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.2657085561497326No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1510.25233957219251335No Hit
ACTTATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1480.24732620320855617No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG1270.21223262032085563No Hit
ACTTATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.2005347593582888No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG1120.18716577540106952No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.1838235294117647No Hit
ACTTATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT980.16377005347593582No Hit
ACTTATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.16042780748663102No Hit
ACTTATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG940.15708556149732622No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.1270053475935829No Hit
ACTTATGGGACATAAATAAATAAATCTTATAAATAGCACTTTTCTCTCTGTCTAAAAGAAGGTT700.11697860962566844No Hit
ACTTATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.11363636363636363No Hit
ACTTATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.11029411764705882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATG150.004522466658.4571736
TGGTTGA150.004522466658.4571734
GGCAAAC150.004522466658.4571736
CTGGTTG150.004522466658.4571733
CTTTGAC150.004522466658.4571736
TTTGACT150.004537691458.40762737
CGAAGTG150.004537691458.40762737
GCAAACT150.004537691458.40762737
TTGATGC150.004537691458.40762737
TGATGCT150.004552954358.35817338
GATGCTG150.004552954358.35817339
AAGTGAA150.004552954358.35817339
CAAACTG150.004552954358.35817338
ATCCTGG150.00458359558.2595153
AAACATC150.00458359558.2595148
AACTGAC150.00458359558.2595156
TCGGCTG150.00458359558.2595150
CGCTCGG150.00458359558.2595147
TCGAACT150.00458359558.2595153
CTGACGG150.00458359558.2595158
Produced by FastQC (version 0.11.5)