FastQCFastQC Report
Fri 17 Jun 2016
SRR1526539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52128
Sequences flagged as poor quality0
Sequence length56
%GC32

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10592.0315377532228363No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA7021.3466850828729282No Hit
ACTTATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.9323204419889504No Hit
ACTTATGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG2420.46424186617556784No Hit
ACTTATGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2310.44313996316758747No Hit
ACTTATGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG1850.35489564149785147No Hit
ACTTATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.3280386740331492No Hit
ACTTATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.2877532228360958No Hit
ACTTATGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.2743247391037446No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC1330.2551411909146716No Hit
ACTTATGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1300.24938612645794966No Hit
ACTTATGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1260.24171270718232044No Hit
ACTTATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.23403928790669123No Hit
ACTTATGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCA1190.2282842234499693No Hit
ACTTATGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA1180.22636586863106198No Hit
ACTTATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.17265193370165746No Hit
ACTTATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.15922344996930632No Hit
ACTTATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1477133210558625No Hit
ACTTATGGGAATTGACGGATCGTAGTGGTCCTGAGTCGGAGACACGCAGGGATGAG730.14003990178023326No Hit
ACTTATGGGACATAAATAAATAAATCTTATAAATAGCACTTTTCTCTCTGTCTAAA690.13236648250460406No Hit
ACTTATGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.13044812768569675No Hit
ACTTATGGGTAAGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.12852977286678943No Hit
ACTTATGGGACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.12277470841006752No Hit
ACTTATGGGAAGCATGGTATCCCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTT640.12277470841006752No Hit
ACTTATGGGATTTATATTAGAAATAATATTCCTTGTAATCAGGCATGTTAAATATA610.11701964395334562No Hit
ACTTATGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.11701964395334562No Hit
ACTTATGGGAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.10742786985880909No Hit
ACTTATGGGAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.10359116022099447No Hit
ACTTATGGGGTTCAGACCACCGAAAGCAGTTCCCAGCCACATCCAAAAAAAAAAAA530.10167280540208717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATG150.00793364850.7051936
TGGTTGA150.00793364850.7051934
CGGAGAC309.334817E-750.7051937
AGCCATA150.00793364850.7051937
ACCCGTT203.869296E-450.7051936
TCGGAGA309.334817E-750.7051936
CCCGTTG203.869296E-450.7051937
CTCAGGT203.869296E-450.7051937
CCTCAGG203.869296E-450.7051936
CTACCCG203.869296E-450.7051934
CTTAGAG150.00799534750.6060632
CTGGTTG150.00799534750.6060633
CCTCCTC150.00799534750.6060633
CCCTCCT150.00799534750.6060632
TTAGAGC150.00799534750.6060633
ACTTAGA150.0080263350.5566431
AGACACG204.0408754E-450.26213540
ACAACAA150.00821410750.26213540
GTTGTAG204.0408754E-450.26213540
ATAATAA150.00824571950.21338746
Produced by FastQC (version 0.11.5)