FastQCFastQC Report
Fri 17 Jun 2016
SRR1526538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446841
Sequences flagged as poor quality0
Sequence length64
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC40510.9065864591655645No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC28630.6407200771639129No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC26240.5872334902124022No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT23350.5225572407187344No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC20030.4482578814388116No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT12560.2810843230589852No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC10670.23878739864963153No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA10580.23677325939204324No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC10080.22558359684988621No Hit
CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC9640.215736693812788No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8850.19805702699617986No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC8830.19760944049449355No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC6750.15106044431912022No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC6630.14837492530900254No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC6450.144346646793826No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT6260.1400945750278063No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTCC6070.13584250326178662No Hit
CATGATGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCCAGAGGTCC5850.13091905174323754No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC5590.12510042722131587No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC5450.12196732170951188No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.11816283644517848No Hit
CATGATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5120.11458214443168824No Hit
CATGATGGGGGGCTGGTGTGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTGAAGGTCCT5070.11346317817747253No Hit
CATGATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG4910.10988248616398226No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCT4870.1089873131606097No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTC4840.10831593340808027No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTCTGAAGGTCCA4570.10227351563531548No Hit
CATGATGGGAGGGCTGGAGAGATGGCTCAGCGGTTAAAAGCGCTCACTGCTCTTCCAGAGGTCC4550.1018259291336292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAC201.9738293E-458.20774558
TGAATTA150.00461948958.20774556
ATCGGTA150.004621548658.2011954
CTAATAC150.004621548658.2011954
GCTAGTC258.65625E-658.00527232
ATTGCGA150.004683664558.00527234
CGTGTAA150.004687827557.9922630
AACCGAC150.004700333357.9532528
CGGTATA150.004721231357.88835520
GATCGAT202.0282582E-457.8883521
TCGGTAT150.00472751457.8689119
CATTACG150.00473380357.84948317
CCATAAC150.00473380357.84948312
CGTCGTT150.00473380357.84948314
CGTAAGT150.00473380357.84948312
CGTCCTA202.0350041E-457.84948310
GATAGGC407.348717E-1057.8494834
TATCCGT150.00473380357.84948313
CATGATG455150.056.3560641
TGATGGG445650.056.3307153
Produced by FastQC (version 0.11.5)