FastQCFastQC Report
Fri 17 Jun 2016
SRR1526537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432979
Sequences flagged as poor quality0
Sequence length64
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC33200.7667808369459027No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT22770.5258915559415123No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC22520.5201176038560762No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC22310.5152674841043099No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC16500.3810808376387769No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT10950.2528991013420974No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCA9010.20809323315911396No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC8950.20670748465860933No Hit
CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC7880.18199496973294318No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC7850.18130209548269086No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC7160.16536598772688746No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6730.1554347901399375No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC6560.151508502721841No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT5470.12633407162934No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTC5250.12125299379415629No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCAAAGGTCC5190.11986724529365167No Hit
CATGATGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCCAGAGGTCC4780.11039796387353659No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC4390.10139059862025641No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTC4350.10046676628658664No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAC150.004591374758.2972334
CGGTACT150.004604107258.2564735
TTACGAA150.004633918458.1615953
AAGCGTA258.518777E-658.16158753
ACTTGCG150.004657441858.08725431
GTCGATG303.7203063E-758.08050532
ACGATAC150.004661728658.0737645
TAAGGCG150.0047393957.8319317
GATGGGG251100.056.313834
ATGATGG437700.056.299732
TGATGGG432100.056.293683
CATGATG441500.056.2344781
ATGGGGG134800.056.2334755
ATGGGAT34300.056.1976625
ATGGGGC43950.055.9573635
GATGGGA127900.055.674554
ATGGGGT29650.055.3617975
ATGGGAC22200.055.0647855
ATGGGGA44850.054.834475
ATGGGAG43450.054.738975
Produced by FastQC (version 0.11.5)