FastQCFastQC Report
Fri 17 Jun 2016
SRR1526536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences396087
Sequences flagged as poor quality0
Sequence length56
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC67731.709977858399796No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC39250.9909439087877158No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC27100.6841931191884612No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC22990.5804280372746391No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC13600.3433589085226226No Hit
CATGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT13010.328463191167597No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC10430.2633259864625701No Hit
CATGATGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT10290.2597914094630725No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC9490.2395938266088006No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTC8360.21106474082714152No Hit
CATGATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC7380.1863227018306584No Hit
CATGATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC6530.16486277004799452No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT6430.16233807219121052No Hit
CATGATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG6400.16158066283417533No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC6340.16006584412010494No Hit
CATGATGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6310.15930843476306972No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC5650.1426454289082954No Hit
CATGATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA5440.13734356340904902No Hit
CATGATGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5090.12850712091030506No Hit
CATGATGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC5030.12699230219623467No Hit
CATGATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC5000.12623489283919948No Hit
CATGATGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTCTTCC4900.12371019498241549No Hit
CATGATGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4770.1204280877685963No Hit
CATGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTC4310.10881447762738994No Hit
CATGATGGGGGGCTGGTGTGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTTCTG4240.10704718912764115No Hit
CATGATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGACTGCTCTTCTG4220.10654224955628434No Hit
CATGATGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC4000.10098791427135957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGACGC150.008138698550.44209731
CGGTAGG150.008138698550.44209731
ATAGATC150.00827556550.2297545
CATAGGA301.0135445E-650.2297544
CCGTACT150.00828391450.21693840
CGTACTT150.00828391450.21693840
ATAGGCG150.00830900150.1785449
TATTACG150.00846070849.94938730
GATAGGG1550.049.7474444
AACGTCG550.049.7097612
TAACGTC550.049.7097611
GGACGAT150.00862306949.709768
GTAACGT550.049.7097610
ACGAATC150.00862306949.7097612
ATGGGGC40200.048.6955685
ATGGGAT31300.048.555415
ATGATGG399900.048.503442
CATGATG402650.048.4872321
TGATGGG394850.048.4623373
GATGGGG234200.048.366754
Produced by FastQC (version 0.11.5)