FastQCFastQC Report
Fri 17 Jun 2016
SRR1526535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41888
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2200.5252100840336135No Hit
GGCTGCGGGCTGGCTGCGGAGTTGCAATACATCTCTGAGCCTGAGTTGTCTACTTCAGGGATCA1790.4273300229182582No Hit
GGCTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1480.3533231474407945No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1420.3389992360580596No Hit
GGCTGCGGGGGTCTCCATCACTTGTTCAGACAGGCCTTGAGCTTATCCAATCTCCTATTACCTA1200.28647822765469827No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1190.2840909090909091No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.27692895339954166No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT980.2339572192513369No Hit
GGCTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.23156990068754776No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT970.23156990068754776No Hit
GGCTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG870.2076967150496562No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC860.20530939648586705No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.1957601222307105No Hit
GGCTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCCC770.1838235294117647No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA770.1838235294117647No Hit
GGCTGCGGGCAAGATATACAATCTATGTGCTGAGAGACATTATGACACCGCCAAATTTAACTGC750.17904889228418638No Hit
GGCTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA680.16233766233766234No Hit
GGCTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG640.15278838808250572No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA630.15040106951871657No Hit
GGCTGCGGGGATAAAGGAAATTTGGCTTGTTGTGTGACACAACAGACATAGTCATGCTGTGTCT620.14801375095492741No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC620.14801375095492741No Hit
GGCTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC600.14323911382734913No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC590.14085179526355998No Hit
GGCTGCGGGAAGACACAAAGGCCTGGGTGGTGATAAATGGTACTTAGAGATGAGATGTCATAAG560.1336898395721925No Hit
GGCTGCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCAC540.1289152024446142No Hit
GGCTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC540.1289152024446142No Hit
GGCTGCGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGCCTCCCGGG530.12652788388082506No Hit
GGCTGCGGGCATTCGTCAATGGCCCAGTGCAGTTCTCTGGAGAAAGCTACCTTTTCCCAAGGCA530.12652788388082506No Hit
GGCTGCGGGGTGCGGGCTTACCGCTCTTCCCTCTACACCAGGACGCCGTGCCTCCACACCAGGA510.12175324675324675No Hit
GGCTGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA500.1193659281894576No Hit
GGCTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT500.1193659281894576No Hit
GGCTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.1193659281894576No Hit
GGCTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG480.1145912910618793No Hit
GGCTGCGGGAGCTCTCCAAGCCTCTCGCTCCTGCACTAGGCTTACAGCCAGGGATGATGTGCAA480.1145912910618793No Hit
GGCTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG460.109816653934301No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT440.10504201680672269No Hit
GGCTGCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.10504201680672269No Hit
GGCTGCGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC440.10504201680672269No Hit
GGCTGCGGGGGATGCCCCAGCTGCTCGCTACCAGACAATTGAAGAGAACATTAAGATCTTTGAG430.10265469824293352No Hit
GGCTGCGGGAGCCAGGCCAGCCACACCACATGGTGAAACCCTCTTCTCAAAAGAGAGACAAAAA430.10265469824293352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCCA150.00462146558.10804435
GAGCGCT150.00462146558.10804436
CCAATCT150.00462146558.10804447
AACAAAG150.00462146558.10804434
TCCCAAG150.00462146558.10804455
CATTGAT258.4309195E-658.10804458
TACAGGA150.00462146558.10804437
TGACTTT258.4309195E-658.10804444
CTCCCTT150.00462146558.10804452
ATCCAAT150.00462146558.10804445
GTCAATA150.00462146558.10804454
TTTCTAC258.4309195E-658.10804444
ATTGGTA258.4309195E-658.10804430
TTCGAGG150.00462146558.10804448
TCAATAA150.00462146558.10804455
TAGCATC258.4309195E-658.10804437
AGCAGTC150.00462146558.10804450
AGGTGGT150.00462146558.10804432
GTATAGC258.4309195E-658.10804434
CAATAAC150.00462146558.10804456
Produced by FastQC (version 0.11.5)