FastQCFastQC Report
Fri 17 Jun 2016
SRR1526534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41038
Sequences flagged as poor quality0
Sequence length64
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2100.5117208440957162No Hit
GGCTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT1770.4313075685949607No Hit
GGCTGCGGGCTGGCTGCGGAGTTGCAATACATCTCTGAGCCTGAGTTGTCTACTTCAGGGATCA1340.32652663385155223No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC1110.27048101759345No Hit
GGCTGCGGGGGTCTCCATCACTTGTTCAGACAGGCCTTGAGCTTATCCAATCTCCTATTACCTA980.23880306057800088No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC960.23392952872947023No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.22174569910814365No Hit
GGCTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT850.2071251035625518No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT820.19981480578975583No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC810.19737803986549052No Hit
GGCTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC730.177883912471368No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA730.177883912471368No Hit
GGCTGCGGGCAAGATATACAATCTATGTGCTGAGAGACATTATGACACCGCCAAATTTAACTGC720.1754471465471027No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT710.17301038062283738No Hit
GGCTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG700.17057361469857205No Hit
GGCTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA680.16570008285004142No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.14133242360738826No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC570.13889565768312295No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA560.13645889175885764No Hit
GGCTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG510.12427506213753108No Hit
GGCTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC510.12427506213753108No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC510.12427506213753108No Hit
GGCTGCGGGAAGACACAAAGGCCTGGGTGGTGATAAATGGTACTTAGAGATGAGATGTCATAAG480.11696476436473512No Hit
GGCTGCGGGGCCTTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCC470.1145279984404698No Hit
GGCTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT440.10721770066767387No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCAGAGGTCCT420.10234416881914324No Hit
GGCTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCCC420.10234416881914324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCAT150.00455329658.3238936
GTTGGCT150.00455329658.3238936
TTGCATC150.00457557858.25214837
TAAAACG150.00457557858.25214837
GAGCTTA201.9451258E-458.25214839
AAAACGT150.00457557858.25214838
AAACGTG150.00457557858.25214839
TGAGCTT201.9451258E-458.25214838
TAGGCGA150.00457557858.25214838
GCCTTGA201.9570101E-458.18058834
TACAGTA150.004597940558.18058826
ATCTTCG150.004597940558.18058827
CCAGTAG303.5936864E-758.18058826
CAGTAGC303.5936864E-758.18058827
CACCGGC150.004597940558.18058825
ATGGTGT150.004597940558.18058827
GCCAGTA303.5936864E-758.18058825
ATCAGAG201.9570101E-458.18058835
TATCTTC150.004597940558.18058826
AAGACCT150.004597940558.18058825
Produced by FastQC (version 0.11.5)