SRR1526533_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526533_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39336
Sequences flagged as poor quality	0
Sequence length	56
%GC	58

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	284	0.7219849501728697	No Hit
GGCTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	216	0.5491153142159854	No Hit
GGCTGCGGGCTGGCTGCGGAGTTGCAATACATCTCTGAGCCTGAGTTGTCTACTTC	177	0.4499694935936546	No Hit
GGCTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	165	0.41946308724832215	No Hit
GGCTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	158	0.4016676835468782	No Hit
GGCTGCGGGGGTCTCCATCACTTGTTCAGACAGGCCTTGAGCTTATCCAATCTCCT	120	0.3050640634533252	No Hit
GGCTGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	99	0.25167785234899326	No Hit
GGCTGCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	98	0.24913565182021558	No Hit
GGCTGCGGGGTGCGGGCTTACCGCTCTTCCCTCTACACCAGGACGCCGTGCCTCCA	97	0.2465934512914379	No Hit
GGCTGCGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT	89	0.22625584706121615	No Hit
GGCTGCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	87	0.2211714460036608	No Hit
GGCTGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT	83	0.21100264388854992	No Hit
GGCTGCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC	81	0.2059182428309945	No Hit
GGCTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	80	0.2033760423022168	No Hit
GGCTGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	77	0.19574944071588368	No Hit
GGCTGCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	77	0.19574944071588368	No Hit
GGCTGCGGGGGGAAAAAAGAAAAGCCCTCCAGTTCCCTTGGCTCTGCTGGAGGGCG	75	0.19066503965832823	No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA	71	0.18049623754321742	No Hit
GGCTGCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTC	68	0.1728696359568843	No Hit
GGCTGCGGGATAATGAAAATAGCAATACAGTAAAAGGGGGTGGGGAAGTAAAAAGA	66	0.16778523489932887	No Hit
GGCTGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	59	0.1499898311978849	No Hit
GGCTGCGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTGCTCTTCCA	56	0.14236322961155176	No Hit
GGCTGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	55	0.13982102908277405	No Hit
GGCTGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	55	0.13982102908277405	No Hit
GGCTGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG	54	0.13727882855399634	No Hit
GGCTGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	51	0.1296522269676632	No Hit
GGCTGCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGGCCCAAGAAACA	50	0.1271100264388855	No Hit
GGCTGCGGGAAGGCAGAAGCTGCTATCTGATTGGTATAGCATCTGACTTTCCCTCT	49	0.12456782591010779	No Hit
GGCTGCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	49	0.12456782591010779	No Hit
GGCTGCGGGACTAGTCGGTTTCCAGGAGACAGAAACAAAGATTCCTCAGAGCAGTC	49	0.12456782591010779	No Hit
GGCTGCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC	48	0.12202562538133008	No Hit
GGCTGCGGGGCTCTTAGAACCTTCCTGCGGTCGTGCTTGCATCTCGCTGCTGCAGC	48	0.12202562538133008	No Hit
GGCTGCGGGGATAAAGGAAATTTGGCTTGTTGTGTGACACAACAGACATAGTCATG	47	0.11948342485255237	No Hit
GGCTGCGGGCAAGATATACAATCTATGTGCTGAGAGACATTATGACACCGCCAAAT	46	0.11694122432377467	No Hit
GGCTGCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	44	0.11185682326621924	No Hit
GGCTGCGGGGGATGCCCCAGCTGCTCGCTACCAGACAATTGAAGAGAACATTAAGA	42	0.10677242220866383	No Hit
GGCTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG	42	0.10677242220866383	No Hit
GGCTGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	42	0.10677242220866383	No Hit
GGCTGCGGGCTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGAGCGTGGCCTTCTCCT	41	0.10423022167988612	No Hit
GGCTGCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG	40	0.1016880211511084	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTTGT	25	1.9155314E-5	50.57678	37
ATATGCT	25	1.9155314E-5	50.57678	34
TTGCATC	15	0.007999067	50.576775	37
CTTGGTC	15	0.007999067	50.576775	35
TGGGGTA	20	3.9062105E-4	50.576775	34
GAACAAC	15	0.007999067	50.576775	37
CTTGCAT	15	0.007999067	50.576775	36
TTGGTCT	15	0.007999067	50.576775	36
TTTGTCG	20	3.9062105E-4	50.576775	35
CTACACC	25	1.9450561E-5	50.44659	33
CCGTGAA	15	0.008081133	50.44659	33
CTCTACA	30	9.609448E-7	50.44659	31
CCATTTG	20	3.9563343E-4	50.44659	32
CGTGCTT	15	0.008081133	50.44659	32
AGCATAT	25	1.9450561E-5	50.44659	31
GCCGTGA	15	0.008081133	50.44659	32
AGCCGTG	15	0.008081133	50.44659	31
CATATGC	25	1.9450561E-5	50.44659	33
GCATATG	25	1.9450561E-5	50.44659	32
ATGGCCA	15	0.0082890345	50.124043	50

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content