FastQCFastQC Report
Fri 17 Jun 2016
SRR1526531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912615
Sequences flagged as poor quality0
Sequence length64
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT49230.5394388652389014No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC45070.4938555688872088No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC43520.47687140798693867No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC36820.40345600280512595No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC33320.3651046717399999No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC23340.2557485905885834No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT19290.2113706217846518No Hit
TAGCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGT16460.18036083123770702No Hit
TAGCATGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAGG13520.14814571314300118No Hit
TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC13240.14507760665779107No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTTCCGAAGGT11690.128093445757521No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC11380.12469661357746695No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG11240.12316256033486189No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC10740.11768379875412961No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC9920.10869862976172866No Hit
TAGCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTGCGGAGTC9900.10847947929849937No Hit
TAGCATGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTCTCTCTAG9630.10552094804490393No Hit
TAGCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAACCGAGACC9170.10048048739063022No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAA150.0046603958.083241
TAGCATG919300.056.9638141
GCATGGG908350.056.9192123
CATGGGG619500.056.7887274
AGCATGG918600.056.7398722
CATGGGA199050.056.4723364
ATGGGGG301650.056.4279175
ATGGGGA144500.056.1303065
ATGGGAG79250.056.109325
ATGGGGC110250.055.9941525
ATGGGGT69350.055.8752675
ATGGGAT45800.055.66845
ATGGGAC37450.055.281635
CATGGGT35850.054.768814
TGGGGGG174000.054.512956
ATGGGAA40400.053.817955
TGGGGCT54450.053.6125876
TGGGGAG76300.052.980626
TGGGGGC61450.052.6741186
TTACGGG508.731149E-1152.31526648
Produced by FastQC (version 0.11.5)