FastQCFastQC Report
Fri 17 Jun 2016
SRR1526530_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526530_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences869335
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC88641.0196299470284758No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC55390.6371536864384846No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC54350.6251905191899556No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC45360.521778140762767No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC27560.31702393208601976No Hit
TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT26760.30782149574099743No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC19110.21982319819172125No Hit
TAGCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC16840.19371128506272037No Hit
TAGCATGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC14360.1651837323931511No Hit
TAGCATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC13420.15437086968774985No Hit
TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC13230.15218529105580703No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC12680.14585861606860417No Hit
TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC11810.13585096654339235No Hit
TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT11780.135505875180454No Hit
TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG11740.1350457533632029No Hit
TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC10080.11595069794728154No Hit
TAGCATGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT10060.11572063703865598No Hit
TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC10000.11503045431277932No Hit
TAGCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA9650.11100438841183202No Hit
TAGCATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA9530.10962402296007868No Hit
TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG8940.1028372261556247No Hit
TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG8890.10226207388406079No Hit
TAGCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCT8860.10191698252112248No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA150.008067938550.5588436
TAATCGC204.1095546E-450.18059550
CCGGATA150.00850121749.8941327
AATCGGT150.008524490549.85962725
TAGCATG874800.049.1618421
GCATGGG865750.049.1035043
CATGGGG583250.049.0139244
AGCATGG875200.049.005682
CATGGGA190750.048.9424324
ATGGGGC103350.048.8994945
ATGGGGG278950.048.684115
ATGGGAC35900.048.611885
ATGGGAT47250.048.526245
ATGGGGA142200.048.442715
ATGGGAG73750.048.339245
ATGGGGT64300.047.6613045
CATGGGT35900.047.1556054
TGGGGGG158200.047.115546
ATGGGAA36800.047.0847475
TGGGGCT53750.046.687536
Produced by FastQC (version 0.11.5)