##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526530_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 869335 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.552148481310425 39.0 38.0 40.0 36.0 40.0 2 38.30014206261107 39.0 38.0 40.0 35.0 40.0 3 38.350626628399866 39.0 38.0 40.0 35.0 40.0 4 38.20324846002979 39.0 38.0 40.0 35.0 40.0 5 38.22293017076271 39.0 38.0 40.0 35.0 40.0 6 38.386707080699615 39.0 38.0 40.0 35.0 40.0 7 38.46249834643722 39.0 38.0 40.0 36.0 40.0 8 38.7000040260659 39.0 39.0 40.0 37.0 40.0 9 38.70340317599084 39.0 39.0 40.0 37.0 40.0 10 38.08829392581686 39.0 38.0 40.0 35.0 40.0 11 38.08655811626128 39.0 38.0 40.0 35.0 40.0 12 37.962970546452176 39.0 38.0 40.0 35.0 40.0 13 37.96208941317214 39.0 38.0 40.0 35.0 40.0 14 37.97380181403027 39.0 38.0 40.0 35.0 40.0 15 37.99644555896173 39.0 38.0 40.0 35.0 40.0 16 37.90771451741848 39.0 38.0 40.0 35.0 40.0 17 37.71006113868647 39.0 38.0 40.0 33.0 40.0 18 37.66497955333674 39.0 38.0 40.0 33.0 40.0 19 37.55883520161963 39.0 38.0 40.0 33.0 40.0 20 37.47209648754508 39.0 37.0 40.0 33.0 40.0 21 37.342519281979904 39.0 37.0 40.0 33.0 40.0 22 37.268190053316616 39.0 37.0 40.0 33.0 40.0 23 37.10326628975021 39.0 36.0 40.0 33.0 40.0 24 36.98327572224746 39.0 36.0 40.0 33.0 40.0 25 36.90245762565639 39.0 36.0 40.0 32.0 40.0 26 36.432122254366845 38.0 36.0 40.0 31.0 40.0 27 36.20567560261579 38.0 35.0 40.0 30.0 40.0 28 35.98048623373038 38.0 35.0 40.0 30.0 40.0 29 35.84476180068673 38.0 35.0 40.0 29.0 40.0 30 35.48807536795366 38.0 35.0 40.0 29.0 40.0 31 34.666349566047614 38.0 33.0 39.0 27.0 40.0 32 34.57667412447446 38.0 33.0 39.0 27.0 40.0 33 34.37828340053029 38.0 33.0 39.0 26.0 40.0 34 34.44471578850501 38.0 33.0 39.0 26.0 40.0 35 34.28671225707006 38.0 33.0 39.0 26.0 40.0 36 33.534209481960346 38.0 33.0 39.0 22.0 40.0 37 33.04099570361253 38.0 33.0 39.0 17.0 40.0 38 32.99735199894172 37.0 32.0 39.0 18.0 40.0 39 32.831526396613505 37.0 32.0 39.0 17.0 40.0 40 32.4539067218046 37.0 32.0 39.0 11.0 40.0 41 31.744607084725683 36.0 31.0 39.0 4.0 40.0 42 31.58729718693024 36.0 30.0 39.0 4.0 40.0 43 31.444785957082136 36.0 30.0 39.0 4.0 40.0 44 30.97974428730006 36.0 30.0 39.0 4.0 40.0 45 30.780466678553147 36.0 29.0 38.0 4.0 40.0 46 30.023182087457656 35.0 29.0 38.0 4.0 40.0 47 29.763346696037775 35.0 28.0 38.0 4.0 40.0 48 29.27891204196311 35.0 27.0 38.0 4.0 40.0 49 29.208457039001075 35.0 27.0 38.0 4.0 40.0 50 28.792911823405248 34.0 26.0 38.0 4.0 40.0 51 27.902390907992892 34.0 23.0 38.0 4.0 39.0 52 27.629820494976045 33.0 23.0 38.0 4.0 39.0 53 27.285319238268332 33.0 20.0 38.0 4.0 39.0 54 26.991274940040377 33.0 17.0 38.0 4.0 39.0 55 26.63625874950393 33.0 4.0 38.0 4.0 39.0 56 16.113330304198037 20.0 4.0 23.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6856259176490838 1 2 -0.45555751535930256 1 3 -1.0148980795561826 1 4 -0.8301180495637865 1 5 -0.39689925508330504 1 6 -0.6085769901861013 1 7 -0.685594280462233 1 8 -1.1269289270431386 1 9 -1.2992395898442055 1 10 -0.462896227862295 1 11 -0.5529313786791832 1 12 -0.6354025261173888 1 13 -0.6969398337299708 1 14 -0.6239563028199555 1 15 -0.6921891260799597 1 16 -0.7787152606493777 1 17 -0.5500886856832352 1 18 -0.6980495116622407 1 19 -0.7308619742005362 1 20 -0.8560199507072639 1 21 -0.6284337867781247 1 22 -0.7121303423072902 1 23 -0.7794285173889861 1 24 -0.7064980984417772 1 25 -0.7087999230981481 1 26 -0.19165725314106652 1 27 -0.43471788933003097 1 28 -0.016004389431110155 1 29 -0.06272828701576572 1 30 -0.10959402242405503 1 31 0.21372498729750333 1 32 0.41545459836248 1 33 0.3282581772040203 1 34 0.5990939904511023 1 35 0.6619904509764467 1 36 0.7597450826688714 1 37 0.7712124986897919 1 38 0.9647035175848231 1 39 0.35613904695895116 1 40 0.8023188184462171 1 41 0.8776587005841598 1 42 0.8219863769921218 1 43 0.9121611401878731 1 44 1.0739872879329688 1 45 0.8602650833807388 1 46 1.367276887805975 1 47 1.313894312741965 1 48 1.4562085677990204 1 49 1.63721196370998 1 50 1.5719183611829273 1 51 1.912921093224039 1 52 2.209881921363589 1 53 2.4332194452408267 1 54 1.9553559386039616 1 55 1.8735439459061674 1 56 2.21892986131261 2 1 -0.05925826183473504 2 2 0.4211672031386584 2 3 0.04764401154604059 2 4 0.031118770024392006 2 5 0.41721667723257383 2 6 -0.2129531634501589 2 7 0.41452967478373637 2 8 0.13039370471217637 2 9 0.16852716607457552 2 10 0.14779320405829566 2 11 0.02578508264254964 2 12 -0.19453015447287925 2 13 0.12283053999021831 2 14 -0.0938215136902798 2 15 -0.010096917691996055 2 16 -0.14583426093893337 2 17 -0.08854897282995466 2 18 -0.06660977636037302 2 19 -0.1702589205419045 2 20 -0.22249640083723676 2 21 -0.22458369129029165 2 22 -0.07940062655846702 2 23 -0.1981093212436349 2 24 -0.23735189306648152 2 25 -0.010178721870957474 2 26 -0.5281989993809262 2 27 -0.1928359819158203 2 28 -0.0766481108213597 2 29 -0.21416190589079775 2 30 -0.013326994844831574 2 31 0.1445003745356388 2 32 0.10491579971980514 2 33 0.2715348251418419 2 34 0.14556582072676605 2 35 0.37685434421226915 2 36 -0.17716950533447573 2 37 0.19079937960491833 2 38 0.1352330765188725 2 39 0.2729654022517707 2 40 0.30944705844439824 2 41 -0.2144781121718431 2 42 -0.4068997501059286 2 43 -0.19817204240373698 2 44 0.3191567747712618 2 45 -0.08551018998775461 2 46 0.2401902264966047 2 47 0.07333003983220365 2 48 0.582450966999982 2 49 0.25912243613888464 2 50 0.19768738798784113 2 51 0.11543175630326985 2 52 0.20939716200269132 2 53 0.566928439967036 2 54 0.46470626345780275 2 55 0.5237249986473351 2 56 0.05600679490057914 3 1 -0.10162534363184506 3 2 0.4267852577310265 3 3 -0.14084431110038764 3 4 0.13143747192761168 3 5 0.2807052666070433 3 6 -0.027347027790753486 3 7 0.3274483044417664 3 8 0.19525129480147996 3 9 0.11977994957218385 3 10 0.32850068875191596 3 11 -0.048225089602794924 3 12 0.024833081867974727 3 13 -0.04144802162929295 3 14 -0.06699324539540186 3 15 0.01700265112307875 3 16 -0.3788600695440323 3 17 -0.2131307813679939 3 18 -0.5262096233346867 3 19 -0.21135028023000046 3 20 -0.5308766910068599 3 21 -0.45175062798734444 3 22 -0.43614709317402856 3 23 -0.36149015249014127 3 24 -0.4468400953042533 3 25 -0.5493747883182465 3 26 -0.02780957644323223 3 27 -0.1408895530994343 3 28 -0.2721856745122011 3 29 -0.2756267153174221 3 30 -0.4237787711773606 3 31 -0.4099007143244222 3 32 -0.07660863485484981 3 33 -0.4328756373036171 3 34 -0.20048104110353648 3 35 -0.23841103494545024 3 36 -0.5671137058286035 3 37 -0.07948757404079743 3 38 -0.15402509945761267 3 39 -0.165799174435584 3 40 -0.3810584902609051 3 41 -0.23172093680653916 3 42 -0.1160213918093902 3 43 -0.14076911997891983 3 44 0.07758957226066343 3 45 -0.0035362805624998828 3 46 0.2655339627315314 3 47 0.06912488176917009 3 48 0.2915634637420901 3 49 0.17864067957470908 3 50 0.0269509594915327 3 51 -0.11084354666167684 3 52 -0.023531100347959466 3 53 0.35868852495421066 3 54 0.08990916728786402 3 55 -0.03722083422239919 3 56 -0.4936587281378646 4 1 -0.0018493369478917998 4 2 0.45761746226482813 4 3 -0.07894297103737813 4 4 0.07074105067677294 4 5 0.19408984146399177 4 6 -0.04977753452692468 4 7 0.208931367772486 4 8 0.12437619328746052 4 9 0.0488191164479872 4 10 0.09196873502204994 4 11 -0.14554917223559727 4 12 -0.32040187730039804 4 13 -0.0866426090030572 4 14 -0.06565427352004605 4 15 -0.2959582537039225 4 16 -0.35852141409625915 4 17 -0.7658253258759515 4 18 -0.38608832090006473 4 19 -0.527366347892503 4 20 -0.8105378796834302 4 21 -0.5057973938013163 4 22 -0.30571969771592933 4 23 -0.5948001564337915 4 24 -0.5668394094513687 4 25 -0.568673753148019 4 26 -0.4849009055481801 4 27 -0.4586342817744722 4 28 -0.6147426899298623 4 29 -0.7810285861370332 4 30 -0.38722281259664015 4 31 -0.2383424098150968 4 32 -0.06239745140011621 4 33 -0.09645077014864256 4 34 -0.008995592875301384 4 35 -0.44628213201389855 4 36 0.2059531632685747 4 37 0.2236375769637391 4 38 0.05315390970319811 4 39 0.21540565085118857 4 40 0.331425746813494 4 41 0.48596954714060914 4 42 0.6106101197012492 4 43 0.6378313348662381 4 44 0.8641116278864516 4 45 0.5460812785182689 4 46 1.1059250859247385 4 47 0.6765863376166088 4 48 1.2231169049309862 4 49 0.8581291580256796 4 50 0.8456925129794826 4 51 0.5031865092820951 4 52 1.0795728515675194 4 53 0.8932905822011037 4 54 0.7271304031635815 4 55 0.5864657185407296 4 56 0.3421169169145628 5 1 -0.09345287290000925 5 2 0.44440346889552984 5 3 -0.05720040529136128 5 4 -0.04654301699555674 5 5 0.22630783916947905 5 6 -0.596399448707075 5 7 0.4021018728498049 5 8 0.13985941584574135 5 9 0.11985894805727071 5 10 -0.28547708634093993 5 11 -0.014543853137098495 5 12 -0.20014221174305646 5 13 -0.15330832752382406 5 14 -0.037334735709563915 5 15 0.005571105633890738 5 16 -0.08238340197895155 5 17 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-0.9434128853716359 120 27 -0.6782433612196215 120 28 -0.6804215465923278 120 29 -0.9657490569230802 120 30 -0.6394296221805007 120 31 -0.5182078995166535 120 32 -0.5402452866250016 120 33 -0.5546895259283815 120 34 -0.3374529278900127 120 35 -0.5688126070033874 120 36 -0.6498686105859832 120 37 -0.3601791527546254 120 38 -0.4239373050929345 120 39 -0.5672761482240531 120 40 -0.21310977231218686 120 41 -0.5794991371805374 120 42 -0.6902726041322964 120 43 -0.2779105734986196 120 44 -0.06434570712826115 120 45 0.003479344932728168 120 46 0.021982252801578284 120 47 -0.20645275443887456 120 48 -0.18688729851584185 120 49 -0.034724982369443325 120 50 -0.04455549038327433 120 51 0.19635590818919724 120 52 0.42503312899500756 120 53 0.06664072932174037 120 54 0.2162855103514758 120 55 -0.10445483631425034 120 56 0.041864710104981384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 347.0 5 42.0 6 82.0 7 141.0 8 221.0 9 346.0 10 380.0 11 729.0 12 812.0 13 1170.0 14 1635.0 15 2264.0 16 2959.0 17 3690.0 18 4817.0 19 5694.0 20 5767.0 21 7369.0 22 7788.0 23 8693.0 24 12310.0 25 17769.0 26 17219.0 27 16298.0 28 18338.0 29 19272.0 30 22327.0 31 26690.0 32 31571.0 33 39880.0 34 52108.0 35 72048.0 36 102764.0 37 139119.0 38 151012.0 39 75664.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 99.41251152474209 0.39963949570836443 0.13248417614064734 0.05536480340890648 8 99.67538405792934 0.25996882674688127 0.030828161755824854 0.03381895356795712 9 99.09298486774374 0.5335112471026704 0.14815922515485977 0.22534465999873468 10 67.36298435010669 22.010387250024444 3.91782224343895 6.708806156429915 11 43.13504000184049 23.115599855061628 15.626197035665193 18.123163107432696 12 37.61415334709864 14.971443689716851 23.414679036274855 23.99972392690965 13 28.363289180810618 15.103613681722235 26.556045713102545 29.977051424364596 14 23.95555223245354 17.114115962200994 33.27842546314137 25.6519063422041 15 27.952515428459684 17.3950203316328 30.030425555165728 24.622038684741785 16 34.918759741641594 17.315304226794044 23.40950266583078 24.35643336573358 17 34.21523348306464 18.318599849310104 23.160116640880673 24.30605002674458 18 35.09464130628584 18.559703681549692 21.919743252025974 24.425911760138497 19 32.0158511966043 22.234926696842987 20.95406258807019 24.795159518482517 20 32.261096125199145 23.446542472119493 21.507704164677598 22.78465723800376 21 30.783529939551496 21.824152944492052 24.912490581881553 22.479826534074896 22 29.367274986052553 19.02902793514583 27.943773113931915 23.6599239648697 23 31.60917623051236 19.30721599203067 25.256144653429697 23.827463124027272 24 32.91074735716011 20.687195886487295 21.32929957553518 25.072757180817412 25 31.029383672464228 19.355892960300856 20.68663937048468 28.928083996750235 26 26.811054571906396 18.244233250500393 24.95819027482197 29.98652190277124 27 25.575471426515044 16.823376997265008 25.90470706779905 31.6964445084209 28 26.643973173158418 21.295763164682903 22.15575137266463 29.90451228949405 29 30.61458408930943 21.441037320164085 21.231495145049493 26.712883445476987 30 31.03959157461223 17.959579445448163 24.989652162784452 26.011176817155157 31 33.066077273665364 17.65619433366423 24.77180045034606 24.505927942324345 32 36.90986183622256 17.431595950272577 22.543713403905507 23.114828809599352 33 36.999230228588644 17.778718994196133 23.179929162564875 22.042121614650352 34 29.800508108145547 19.266902213998712 30.74977693798472 20.18281273987102 35 28.152485896971108 26.367295289599895 26.113867959165294 19.366350854263697 36 29.700897146869686 26.58767739670338 22.50997856456501 21.201446891861927 37 31.41165450325756 25.585800967480353 20.934811583666544 22.06773294559554 38 32.056658075104885 24.904240687016724 21.050636276424832 21.988464961453555 39 33.00353340553486 21.153274901724632 21.26712522726721 24.5760664654733 40 31.161040013897733 19.983785974868262 21.248016677282994 27.60715733395101 41 26.123430459041575 22.452185097110284 22.886960665476114 28.53742377837203 42 24.765627982798257 19.487210339557414 25.674076250880738 30.07308542676359 43 25.442596457839162 17.54803935528459 26.966288232658776 30.043075954217475 44 27.765643782440424 17.37196962151971 25.08424955935454 29.778137036685326 45 28.753543701013545 18.561350034278938 23.68581963719913 28.999286627508386 46 26.639873673625196 21.31050869999919 23.763005974551675 28.286611651823932 47 25.913776727592836 19.83563833483911 25.794125141890795 28.45645979567725 48 27.339296091420195 20.2530015809699 27.323405198062005 25.0842971295479 49 31.335440528174647 18.18846948548424 24.637946667717944 25.838143318623164 50 26.226468910439248 18.91426544042965 25.133800824602652 29.72546482452845 51 24.593225447463603 20.936775479922098 27.390916257071318 27.079082815542982 52 25.01742548214366 20.857345319126974 26.236687337560294 27.88854186116907 53 26.43241355870074 18.900446489161414 28.546073992615355 26.121065959522493 54 25.309272908470533 18.00517668842763 28.141601339302806 28.54394906379903 55 27.498752741069044 17.798907053104223 24.750954298112287 29.95138590771444 56 25.32074709883118 19.7589262716761 22.2914882804614 32.62883834903132 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 2.5 5 4.0 6 24.0 7 44.0 8 114.0 9 184.0 10 307.5 11 431.0 12 512.5 13 619.0 14 644.0 15 1016.0 16 1388.0 17 1722.5 18 2057.0 19 2123.5 20 2190.0 21 2537.5 22 3468.0 23 4051.0 24 4974.5 25 5898.0 26 6580.5 27 7263.0 28 8222.0 29 9338.0 30 9495.0 31 8745.0 32 7995.0 33 8620.5 34 9246.0 35 9246.5 36 9247.0 37 9597.0 38 10100.0 39 10253.0 40 11182.5 41 12112.0 42 13028.5 43 13945.0 44 15231.5 45 16518.0 46 18305.0 47 21337.5 48 22583.0 49 25894.0 50 29205.0 51 31639.0 52 34073.0 53 38415.0 54 47901.0 55 53045.0 56 60206.5 57 67368.0 58 82974.5 59 98581.0 60 98507.0 61 98433.0 62 85116.0 63 63663.5 64 55528.0 65 49615.5 66 43703.0 67 37147.5 68 30592.0 69 26647.5 70 22703.0 71 19119.0 72 13639.0 73 11743.0 74 9252.5 75 6762.0 76 5436.5 77 4111.0 78 3308.5 79 1970.5 80 1435.0 81 1073.0 82 711.0 83 508.0 84 305.0 85 210.5 86 116.0 87 79.5 88 33.0 89 23.0 90 13.5 91 4.0 92 5.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.06349681078065418 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 2.300609086255586E-4 24 5.751522715638966E-4 25 0.040145628555159976 26 0.05958577533401969 27 0.11042923614026814 28 0.10939396205145312 29 0.1440181287995997 30 0.23052103044280972 31 0.2302909695341842 32 0.300229485756354 33 0.32714661206554435 34 0.3432508756693335 35 0.3691327278897088 36 0.40019095055415915 37 1.2679806978897663 38 0.47680123312647027 39 0.478986811758413 40 0.6769542236307062 41 0.5465096884400145 42 0.5756123933811477 43 0.6340478641720395 44 0.6705125181891907 45 0.6707425790978162 46 0.7101980249270995 47 0.6896075736051119 48 0.8289094537778877 49 0.7929049215779878 50 0.7889938861313534 51 0.7701288916240574 52 0.8157959819862309 53 0.8369615855797822 54 0.8505351791886903 55 0.856401732358642 56 0.8559416105413908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 869335.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.34933299611422 #Duplication Level Percentage of deduplicated Percentage of total 1 67.30753621219786 21.77353902076405 2 11.75095311787862 7.6027099086396435 3 5.862133535831964 5.6890832945495 4 3.6123835139487275 4.674327888096024 5 2.466588950794741 3.989625366689754 6 1.7641822425729643 3.424207129849463 7 1.2780433390540205 2.8940694690966957 8 0.9560835718136996 2.4742932669372526 9 0.6902041385523602 2.0094879161993813 >10 3.883228383208942 22.560789883770692 >50 0.2586509103148493 5.7789978170659975 >100 0.15112185229444006 9.00435796192185 >500 0.012714578919002106 2.9165273145958586 >1k 0.005085831567600842 2.876689182609075 >5k 0.0010898210502001805 2.331294579214789 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 8864 1.0196299470284758 No Hit TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 5539 0.6371536864384846 No Hit TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 5435 0.6251905191899556 No Hit TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 4536 0.521778140762767 No Hit TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 2756 0.31702393208601976 No Hit TAGCATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 2676 0.30782149574099743 No Hit TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 1911 0.21982319819172125 No Hit TAGCATGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGC 1684 0.19371128506272037 No Hit TAGCATGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTTC 1436 0.1651837323931511 No Hit TAGCATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 1342 0.15437086968774985 No Hit TAGCATGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 1323 0.15218529105580703 No Hit TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC 1268 0.14585861606860417 No Hit TAGCATGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC 1181 0.13585096654339235 No Hit TAGCATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTCTT 1178 0.135505875180454 No Hit TAGCATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG 1174 0.1350457533632029 No Hit TAGCATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC 1008 0.11595069794728154 No Hit TAGCATGGGGGCACACAGCCATCCATCCTCCCCTTTCCCTCTTCTCCCCCGTTCTT 1006 0.11572063703865598 No Hit TAGCATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTC 1000 0.11503045431277932 No Hit TAGCATGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACGGTAA 965 0.11100438841183202 No Hit TAGCATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA 953 0.10962402296007868 No Hit TAGCATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG 894 0.1028372261556247 No Hit TAGCATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG 889 0.10226207388406079 No Hit TAGCATGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCT 886 0.10191698252112248 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 8.052131801894552E-4 0.0 0.0 0.0 0.0 9 0.0010352740888150138 0.0 0.0 0.0 0.0 10 0.0020705481776300276 0.0 0.0 0.0 0.0 11 0.005176370444075069 0.0 0.0 0.0 0.0 12 0.006211644532890082 0.0 0.0 0.0 0.0 13 0.008052131801894551 0.0 0.0 0.0 0.0 14 0.009662558162273462 0.0 0.0 0.0 0.0 15 0.010582801796775697 0.0 0.0 0.0 0.0 16 0.012078197702841827 0.0 0.0 0.0 0.0 17 0.013458563154595178 0.0 0.0 0.0 0.0 18 0.01518401996928687 0.0 0.0 0.0 0.0 19 0.017484629055542454 0.0 0.0 0.0 0.0 20 0.019325116324546924 0.0 0.0 0.0 0.0 21 0.020590451321987495 0.0 0.0 0.0 0.0 22 0.022891060408243083 0.0 0.0 0.0 0.0 23 0.02507663904018589 0.0 0.0 0.0 0.0 24 0.027492278580754256 0.0 0.0 0.0 0.0 25 0.02841252221525649 0.0 0.0 0.0 0.0 26 0.029562826758384283 0.0 0.0 0.0 0.0 27 0.030943192210137634 0.0 0.0 0.0 0.0 28 0.03496925811108491 0.0 0.0 0.0 0.0 29 0.03680974538008938 0.0 0.0 0.0 0.0 30 0.03842017174046829 0.0 0.0 0.0 0.0 31 0.0394554458292833 0.0 0.0 0.0 0.0 32 0.041295933098287774 0.0 0.0 0.0 0.0 33 0.04302138991297946 0.0 0.0 0.0 0.0 34 0.04463181627335837 0.0 0.0 0.0 0.0 35 0.04842782126568009 0.0 0.0 0.0 0.0 36 0.051188552169186795 0.0 0.0 0.0 0.0 37 0.05302903943819126 0.0 0.0 0.0 0.0 38 0.055444678978759626 0.0 0.0 0.0 0.0 39 0.05809037942795355 0.0 0.0 0.0 0.0 40 0.06039098851420914 0.0 0.0 0.0 0.0 41 0.06246153669183917 0.0 0.0 0.0 0.0 42 0.06545232850397142 0.0 0.0 0.0 0.0 43 0.06821305940747813 0.0 0.0 0.0 0.0 44 0.0712038512196104 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGTA 15 0.0080679385 50.55884 36 TAATCGC 20 4.1095546E-4 50.180595 50 CCGGATA 15 0.008501217 49.89413 27 AATCGGT 15 0.0085244905 49.859627 25 TAGCATG 87480 0.0 49.161842 1 GCATGGG 86575 0.0 49.103504 3 CATGGGG 58325 0.0 49.013924 4 AGCATGG 87520 0.0 49.00568 2 CATGGGA 19075 0.0 48.942432 4 ATGGGGC 10335 0.0 48.899494 5 ATGGGGG 27895 0.0 48.68411 5 ATGGGAC 3590 0.0 48.61188 5 ATGGGAT 4725 0.0 48.52624 5 ATGGGGA 14220 0.0 48.44271 5 ATGGGAG 7375 0.0 48.33924 5 ATGGGGT 6430 0.0 47.661304 5 CATGGGT 3590 0.0 47.155605 4 TGGGGGG 15820 0.0 47.11554 6 ATGGGAA 3680 0.0 47.084747 5 TGGGGCT 5375 0.0 46.68753 6 >>END_MODULE