FastQCFastQC Report
Fri 17 Jun 2016
SRR1526529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89797
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC4390.4888804748488257No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3930.4376538191699054No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3800.42317672082586283No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3560.39644977003686094No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3130.3485639832065659No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.3207234094680223No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2670.2973373275276457No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2610.29065558983039524No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2420.2694967537891021No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG2040.22717908170651582No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1970.2193837210597236No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1950.21715647516064013No Hit
CCGCTAGGGAGATTAAGTAAGTTCTGCATATTTCTAAGTAGCCACAATTCCTTATTCCGCACTA1790.1993385079679722No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1710.19042952437163824No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1700.1893159014220965No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1670.18597503257347128No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1650.1837477866743878No Hit
CCGCTAGGGCACTTGAAACTAGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGCCAGTAGA1620.18040691782576257No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1550.17261155717897037No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1550.17261155717897037No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1510.16815706538080336No Hit
CCGCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.15924808178446942No Hit
CCGCTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1430.15924808178446942No Hit
CCGCTAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC1350.15033909818813548No Hit
CCGCTAGGGGGGTGCGGAGTTTGAGCTTGGCGAGCAGCTGGACAACGAGCGAGTTGGGATGGCT1340.14922547523859372No Hit
CCGCTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1310.14588460638996847No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA1290.143657360490885No Hit
CCGCTAGGGATGCTGGGGTTTTACATGCGTTTGCACGTCTTAGTAGTTTCTCATTGAGGTAATG1270.1414301145918015No Hit
CCGCTAGGGGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACC1260.14031649164225976No Hit
CCGCTAGGGATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTAACAGTGACCAAA1110.12361214739913359No Hit
CCGCTAGGGGGGGGCTGGTGAAATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT1110.12361214739913359No Hit
CCGCTAGGGGGGGTGATCCTGTTGGCCGTTGGAGTCTGGGGAAAGCTGACTTTGGGAACCTATA1090.12138490150005012No Hit
CCGCTAGGGAGGACGTTCCCTTCACAGAGAAAGATTTTGAGAACGGTCCTCAGAACATATACAA1060.11804403265142488No Hit
CCGCTAGGGGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCCAA1050.11693040970188315No Hit
CCGCTAGGGGTTCTGATTGGTTTCTATTTGCTTGTTAAAGAACCCTCCTTAGATGGCTATGCAT1050.11693040970188315No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTGGC1030.11470316380279966No Hit
CCGCTAGGGAGGGAGCCATGGCTGAGTCCAGGTTACAGAGCCTGCCCCGCCCTTAGAATGTTAC1020.11358954085325791No Hit
CCGCTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC1000.11136229495417442No Hit
CCGCTAGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT990.11024867200463268No Hit
CCGCTAGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA980.10913504905509092No Hit
CCGCTAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC960.10690780315600744No Hit
CCGCTAGGGCAGCAACCTTAAGAAATTAGTAGAGAAGTTCGGGAAGGAGTGGGCCCATGCCACT960.10690780315600744No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC950.10579418020646571No Hit
CCGCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT950.10579418020646571No Hit
CCGCTAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC940.10468055725692395No Hit
CCGCTAGGGGGTGGCGCGGAGAACTCTGCAAAACAAGAGGCTGGGGATTGCGTTAGCGAGAAAC900.10022606545875698No Hit
CCGCTAGGGGACCTTTCCAATTGACCTGCAGCATAGATAATCTGTGATCGACTCCATTGCGAAA900.10022606545875698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTCT150.004599055758.23443658
CGCACTA201.9607945E-458.23443658
GTCATCT201.9717024E-458.1691950
ATTAAGC258.457269E-658.1691951
CCATGCA258.457269E-658.1691957
CGTTAGC201.9717024E-458.1691951
ATTCCGC201.9717024E-458.1691954
TATTCCG201.9717024E-458.1691953
AGACACG201.9717024E-458.1691955
CGAGAAA201.9717024E-458.1691957
CTATGCA150.0046195358.16918657
CGTTGAA150.0046195358.16918650
GCGTTGA150.0046195358.16918649
GTTGTTT150.0046195358.16918648
TAGATGG150.0046195358.16918650
CGATGCA150.0046195358.16918648
ACACTCT150.0046195358.16918656
GACTGTT150.0046195358.16918645
CGCGTCA150.0046195358.16918649
CATTTGG150.0046195358.16918655
Produced by FastQC (version 0.11.5)