FastQCFastQC Report
Fri 17 Jun 2016
SRR1526528_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526528_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87034
Sequences flagged as poor quality0
Sequence length64
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3480.39984373922834754No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3410.3918009053932946No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG3100.35618264126663146No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3050.35043775995587934No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2730.3136705195670657No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.2665624928188984No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2150.24702989636234116No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.23324218121653606No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1970.2263483236436335No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG1760.20221982213847461No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC1570.18038927315761655No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1370.1574097479146081No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC1360.15626077165245766No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA1330.15281384286600638No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC1330.15281384286600638No Hit
CCGCTAGGGCACTTGAAACTAGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAGCCAGTAGA1300.14936691407955513No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG1280.14706896155525426No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCCAAGGCCCT1280.14706896155525426No Hit
CCGCTAGGGAGATTAAGTAAGTTCTGCATATTTCTAAGTAGCCACAATTCCTTATTCCGCACTA1270.14591998529310385No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG1240.1424730565066526No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTGGC1220.14017510398235172No Hit
CCGCTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT1130.1298343176229979No Hit
CCGCTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1090.12523841257439622No Hit
CCGCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.12523841257439622No Hit
CCGCTAGGGATGCTGGGGTTTTACATGCGTTTGCACGTCTTAGTAGTTTCTCATTGAGGTAATG1050.12064250752579452No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC1030.11834455500149368No Hit
CCGCTAGGGGGGTGCGGAGTTTGAGCTTGGCGAGCAGCTGGACAACGAGCGAGTTGGGATGGCT1020.11719557873934325No Hit
CCGCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT990.11374864995289198No Hit
CCGCTAGGGGAACATCAAGGCCTTGTTCCCCACAGAGATCCATTCTGGGCTCCTGGAAGTCATC940.10800376864213986No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCTCGGAAGGA930.10685479237998942No Hit
CCGCTAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTC900.10340786359353815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGCAT150.004578378558.29913336
TCCGGCT150.004588904358.26535433
ATGATTT201.9553043E-458.26535433
TGAACGT201.9553043E-458.26535437
GTAAAGT258.372537E-658.26535433
AACGTAC201.9665422E-458.19791839
GAACGTA201.9665422E-458.19791838
CAAGAAT150.0046100158.19791839
ACGACCC150.0046100158.19791839
CGGAAAC150.004641803458.09705458
CATGATT150.004641803458.09705432
ATCAAAC303.6781967E-758.09705431
GAACGTC150.004641803458.09705450
TATACAA150.004641803458.09705458
CATATGG150.004652437858.0635151
AGCCACT201.989172E-458.0635147
ATTCGAA150.004652437858.0635146
CATTCGA150.004652437858.0635145
CCGTCAT201.989172E-458.0635156
ATCAATC201.989172E-458.0635154
Produced by FastQC (version 0.11.5)