FastQCFastQC Report
Fri 17 Jun 2016
SRR1526527_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526527_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82752
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC5480.6622196442382058No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3820.46162026295436964No Hit
CCGCTAGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT3700.4471191028615622No Hit
CCGCTAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC3470.41932521268368134No Hit
CCGCTAGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT3450.4169083526682134No Hit
CCGCTAGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT2570.31056651198762564No Hit
CCGCTAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG2480.2996906419180201No Hit
CCGCTAGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2350.28398105181747874No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2190.2646461716937355No Hit
CCGCTAGGGAGGGTCTGCAACCTCATGGGCAGTGCTTGGAGCTGAGGTAGGTGTGT2020.24410286156225833No Hit
CCGCTAGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2000.2416860015467904No Hit
CCGCTAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC1940.2344354215003867No Hit
CCGCTAGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.20180781129156997No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1650.1993909512761021No Hit
CCGCTAGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1510.18247293116782676No Hit
CCGCTAGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG1500.1812645011600928No Hit
CCGCTAGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA1500.1812645011600928No Hit
CCGCTAGGGGACGTGATCTCAGCACCATCCAGTTAAAAGGAGGTGCAGGGCCCTGT1380.16676334106728538No Hit
CCGCTAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG1350.16313805104408352No Hit
CCGCTAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG1310.15830433101314773No Hit
CCGCTAGGGAGATTAAGTAAGTTCTGCATATTTCTAAGTAGCCACAATTCCTTATT1280.15467904098994587No Hit
CCGCTAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1250.151053750966744No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGCCC1240.14984532095901004No Hit
CCGCTAGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1230.1486368909512761No Hit
CCGCTAGGGCACTTGAAACTAGAGAATCTGTTTGCCAAACTTCTTGATCTTGGGAG1210.1462200309358082No Hit
CCGCTAGGGATGCTGGGGTTTTACATGCGTTTGCACGTCTTAGTAGTTTCTCATTG1190.1438031709203403No Hit
CCGCTAGGGGGGTGCGGAGTTTGAGCTTGGCGAGCAGCTGGACAACGAGCGAGTTG1150.1389694508894045No Hit
CCGCTAGGGGACAAGTCTTTCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCG1060.1280935808197989No Hit
CCGCTAGGGGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGATATCTGTCG1030.12446829079659706No Hit
CCGCTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGC1030.12446829079659706No Hit
CCGCTAGGGAGGGAGCCATGGCTGAGTCCAGGTTACAGAGCCTGCCCCGCCCTTAG1020.1232598607888631No Hit
CCGCTAGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT1010.12205143078112916No Hit
CCGCTAGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA980.11842614075792732No Hit
CCGCTAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT970.11721771075019335No Hit
CCGCTAGGGAGGTGGCTTTCAGGAACCTGTGAACACCCTTCCTGGGCCCGCGTCAG950.11480085073472546No Hit
CCGCTAGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA920.1111755607115236No Hit
CCGCTAGGGAGGGTCTGCAACCTCATGGGCAGTGCTTGGAGCTGAGGTAGGGGTGT890.10755027068832174No Hit
CCGCTAGGGGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACATTGA880.10634184068058779No Hit
CCGCTAGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA880.10634184068058779No Hit
CCGCTAGGGGACCTTTCCAATTGACCTGCAGCATAGATAATCTGTGATCGACTCCA870.10513341067285382No Hit
CCGCTAGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC860.10392498066511989No Hit
CCGCTAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGC850.10271655065738593No Hit
CCGCTAGGGGGGGTGATCCTGTTGGCCGTTGGAGTCTGGGGAAAGCTGACTTTGGG830.10029969064191803No Hit
CCGCTAGGGGTTCTGATTGGTTTCTATTTGCTTGTTAAAGAACCCTCCTTAGATGG830.10029969064191803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAG150.00803896450.56353437
CGTCTTA150.00805850650.53251636
ATCCGGT150.00805850650.53251635
GTCGTAG150.00805850650.53251635
ACGTCTT150.00805850650.53251635
TAAGTAG203.9489684E-450.53251335
GCTTGGA600.050.50153434
ATTTTGA150.00807808350.50153434
TGTCGTA150.00807808350.50153434
CATCAAT150.00807808350.50153434
AACACCC251.972974E-550.40881332
TAGGTCA150.00813702750.4088133
CAACACT150.00813702750.4088133
CACAAAT203.9970747E-450.4088132
TCTAGGT150.00815674650.3779831
GTACCCG150.008295773550.16321640
TCTTAAG150.008295773550.16321640
ATCACGT150.008295773550.16321640
TGAAGGT150.00831577750.13268338
CTTAGTA150.00831577750.13268339
Produced by FastQC (version 0.11.5)