FastQCFastQC Report
Fri 17 Jun 2016
SRR1526526_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526526_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77989
Sequences flagged as poor quality0
Sequence length64
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12531.6066368333995822No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11181.4335354985959559No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.7885727474387414No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.36287168703278666No Hit
TACTTCGGGGTGGGGGCATGAGATGTATGTGAGAGGTTTATTACTGTGTGTGTGTGTGTGTGTG2660.3410737411686263No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.32825142007206143No Hit
TACTTCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCAC2440.3128646347561836No Hit
TACTTCGGGGCTTACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAA1710.21926169075125979No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1670.21413276231263384No Hit
TACTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.1923348164484735No Hit
TACTTCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1440.18464142379053455No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.1782302632422521No Hit
TACTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.17438356691328263No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.16412571003603071No Hit
TACTTCGGGCAGTCTGTGCTGCATGTCTGTCACCCATACGCAGTCTCAATCATGGCCTTCACTG1250.16027901370706124No Hit
TACTTCGGGATCACTGACGTGTCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCAGCGCCTCC1210.1551500852684353No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.15130338893946582No Hit
TACTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.1423277641718704No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1090.1397632999525574No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT1040.13335213940427496No Hit
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.13078767518496198No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1010.12950544307530548No Hit
TACTTCGGGGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCTATTGTCATA1010.12950544307530548No Hit
TACTTCGGGATACTTAAACTTTTACTCAGGAATGTAATGCAGCCAACCTTTTGCTTTTAAAGTG1000.12822321096564898No Hit
TACTTCGGGAGACTCTAATGATAAACCCAAGTCTATAGATATACCACTTGAAAGGGATGCTTTA930.11924758619805356No Hit
TACTTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG920.11796535408839708No Hit
TACTTCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCATGGCTCAG920.11796535408839708No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.11668312197874059No Hit
TACTTCGGGATGGCATTCTGGGAGGAATGGAGGTGGAGCAAGCCGTTTATTGACACTGCTGACT900.11540088986908409No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTGCGATCGGAAGAGCTCGTATGCCGTCTTCTGC900.11540088986908409Illumina Single End Adapter 2 (96% over 31bp)
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGC890.11411865775942762No Hit
TACTTCGGGGGATGCCCCAGCTGCTCGCTACCAGACAATTGAAGAGAACATTAAGATCTTTGAG830.10642526510148867No Hit
TACTTCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.1038608008821757No Hit
TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC810.1038608008821757No Hit
TACTTCGGGGAATTTCTATAATATTCATGTCAGCCTTGTGCCACAGCCCAGTGCTACTGGAGAA780.10001410455320622No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC150.004581302458.28395558
ACATTCT150.004593056658.2462854
CATTCTT150.004593056658.2462855
GAGCTAG150.00460483358.20865248
ATGCTTC150.00460483358.20865242
AAGGTAA150.00460483358.20865243
TAACATT150.00460483358.20865252
CAAGGTA150.00460483358.20865242
TACTGAG351.569424E-858.20865244
CGTACTG351.569424E-858.20865242
CATAACA150.00460483358.20865250
ATAACAT150.00460483358.20865251
GTAAGCT150.00460483358.20865246
TACGTAC351.569424E-858.20865240
TCTACGT351.5776095E-858.17107838
CTCTACG351.5776095E-858.17107837
ACTCTAC351.585613E-858.13354536
AACTCTA406.9485395E-1058.09606635
TGAACTC150.00466405458.02124833
CCCATAC150.00466405458.02124833
Produced by FastQC (version 0.11.5)