FastQCFastQC Report
Fri 17 Jun 2016
SRR1526525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75765
Sequences flagged as poor quality0
Sequence length64
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9621.2697155678743484No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9091.1997624232825184No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4620.6097802415363295No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2200.2903715435887283No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.28113244901999607No Hit
TACTTCGGGGTGGGGGCATGAGATGTATGTGAGAGGTTTATTACTGTGTGTGTGTGTGTGTGTG2040.26925361314591173No Hit
TACTTCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGGCCAATCAC1870.24681581205041905No Hit
TACTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.16630370223718074No Hit
TACTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.16234409027915264No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAGACACGCAG1110.1465056424470402No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.14518577179436415No Hit
TACTTCGGGGCTTACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAA1100.14518577179436415No Hit
TACTTCGGGCAGTCTGTGCTGCATGTCTGTCACCCATACGCAGTCTCAATCATGGCCTTCACTG1090.14386590114168812No Hit
TACTTCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.14254603048901207No Hit
TACTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.14122615983633602No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.13462680657295586No Hit
TACTTCGGGGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCTATTGTCATA970.12802745330957566No Hit
TACTTCGGGATCACTGACGTGTCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCAGCGCCTCC970.12802745330957566No Hit
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.12670758265689963No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG870.11482874678281528No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA850.1121890054774632No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT840.11086913482478716No Hit
TACTTCGGGAGACTCTAATGATAAACCCAAGTCTATAGATATACCACTTGAAAGGGATGCTTTA830.10954926417211114No Hit
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGGC800.10558965221408301No Hit
TACTTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGACACGCAG780.10294991090873094No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGA150.00456791958.32533333
GAGGTTT201.9437328E-458.32533333
AGGTTTA201.9437328E-458.32533334
CAATTGA150.00457999958.28647636
TAGATAT201.9501551E-458.28647636
TCAGTAC303.5898847E-758.28647636
AGATATA150.00459210358.2476737
CAGTACG303.606474E-758.2476737
GTCGAGG150.004604230658.20891629
CAAGTGA150.004604230658.20891630
AGGAGCG150.004604230658.20891639
ATAGATA150.004604230658.20891635
CGCGATT201.9630503E-458.20891630
TAGCATA150.004604230658.20891630
AGTGCGA406.82121E-1058.20891630
GTAGCAT150.004604230658.20891629
ATATACC150.004604230658.20891639
GATATAC150.004604230658.20891638
TACCTGA150.004604230658.20891630
TTATTAC303.6231359E-758.20891638
Produced by FastQC (version 0.11.5)