FastQCFastQC Report
Fri 17 Jun 2016
SRR1526524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67513
Sequences flagged as poor quality0
Sequence length56
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA14422.1358849406780918No Hit
TACTTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT12321.8248337357249715No Hit
TACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4800.7109741827499889No Hit
TACTTCGGGGTGGGGGCATGAGATGTATGTGAGAGGTTTATTACTGTGTGTGTGTG3130.4636144150015553No Hit
TACTTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2420.3584494838031194No Hit
TACTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.3021640276687453No Hit
TACTTCGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTGG1830.2710589071734333No Hit
TACTTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.24439737532030867No Hit
TACTTCGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT1600.2369913942499963No Hit
TACTTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG1530.22662302075155896No Hit
TACTTCGGGGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.1984802926843719No Hit
TACTTCGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.18959311539999704No Hit
TACTTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.1792247419015597No Hit
TACTTCGGGGCTTACTTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA1160.17181876083124734No Hit
TACTTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1090.16145038733280997No Hit
TACTTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.15848799490468501No Hit
TACTTCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG980.14515722897812275No Hit
TACTTCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.14367603276406027No Hit
TACTTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC920.13627005169374787No Hit
TACTTCGGGGAGTGCAGTGCTTACTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA910.1347888554796854No Hit
TACTTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG900.1333076592656229No Hit
TACTTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.1333076592656229No Hit
TACTTCGGGCAGTCTGTGCTGCATGTCTGTCACCCATACGCAGTCTCAATCATGGC890.13182646305156043No Hit
TACTTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC870.1288640706234355No Hit
TACTTCGGGATCACTGACGTGTCTCTCGGTCGAGGAAGCGCGCTCCGGAGCTAGCA850.12590167819531053No Hit
TACTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.12293928576718557No Hit
TACTTCGGGGGCTCAGACCCTGGAGGCTAAGGACTTGTTTCGGAAGGAGCTGACTG790.11701450091093568No Hit
TACTTCGGGGATTAGAAGCACAGAATGATCAGGCCTTTAGAGCGATGGAATGGCTA790.11701450091093568No Hit
TACTTCGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.11257091226874824No Hit
TACTTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC750.11108971605468577No Hit
TACTTCGGGAGACTCTAATGATAAACCCAAGTCTATAGATATACCACTTGAAAGGG740.1096085198406233No Hit
TACTTCGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAA730.10812732362656081No Hit
TACTTCGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCC730.10812732362656081No Hit
TACTTCGGGATGGCATTCTGGGAGGAATGGAGGTGGAGCAAGCCGTTTATTGACAC700.10368373498437337No Hit
TACTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA690.1022025387703109No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATAGGA150.00798439750.6402735
CCATACG150.00798439750.6402734
TTGTGCC309.460964E-750.6402736
CCAATAG150.00803215950.56400733
TAAGAGA150.00803215950.56400733
CCCATAC150.00803215950.56400733
GCCAATA150.0080561250.52596332
GGTAAAG150.0080561250.52596332
ACAGCCC301.012093E-650.14861743
ACTGTGT355.0697054E-850.14861743
TCAGGAG150.00829866250.14861745
ACGCAGT150.00829866250.14861738
TTATTAC355.0697054E-850.14861738
TCAATCA150.00829866250.14861746
TGATAAC150.00829866250.14861748
ATTACTG355.0697054E-850.14861740
TATTACT355.0697054E-850.14861739
ATAACAC150.00832321150.11119550
AACTCCT150.00832321150.11119542
TCATGGC150.00832321150.11119550
Produced by FastQC (version 0.11.5)